IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Overexpressed NLRC3 acts as an anti-inflammatory cytosolic protein. Overexpressed NLRC3 acts as an anti-inflammatory cytosolic protein. Journal of innate immunity 2014 imex curation Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. email sent for species in Fig 2 and Supp Fig 2 (NODs) 12/4/17 dsp=83 nesrin.ozoren@boun.edu.tr 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. email sent for species in Fig 2 and Supp Fig 2 (NODs) 12/4/17 nesrin.ozoren@boun.edu.tr Accepted 2017-MAY-31 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of innate immunity 2014 imex curation I think you mean there is a recip. interaction in Fig 2A - only this involves NLRP3 (not NLRC3)- which I could find no origin for - added to comment at Publication level. Again Caspase-1 I could find not origin for.; removed interaction with caspase-1 Overexpressed NLRC3 acts as an anti-inflammatory cytosolic protein. Overexpressed NLRC3 acts as an anti-inflammatory cytosolic protein. Journal of innate immunity 2014 imex curation Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. email sent for species in Fig 2 and Supp Fig 2 (NODs) 12/4/17 dsp=83 nesrin.ozoren@boun.edu.tr 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. email sent for species in Fig 2 and Supp Fig 2 (NODs) 12/4/17 nesrin.ozoren@boun.edu.tr Accepted 2017-MAY-31 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of innate immunity 2014 imex curation I think you mean there is a recip. interaction in Fig 2A - only this involves NLRP3 (not NLRC3)- which I could find no origin for - added to comment at Publication level. Again Caspase-1 I could find not origin for.; removed interaction with caspase-1 Overexpressed NLRC3 acts as an anti-inflammatory cytosolic protein. Overexpressed NLRC3 acts as an anti-inflammatory cytosolic protein. Journal of innate immunity 2014 imex curation Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. email sent for species in Fig 2 and Supp Fig 2 (NODs) 12/4/17 dsp=83 nesrin.ozoren@boun.edu.tr 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. email sent for species in Fig 2 and Supp Fig 2 (NODs) 12/4/17 nesrin.ozoren@boun.edu.tr Accepted 2017-MAY-31 AT 11:32 BST AT 11:32 BST by ORCHARD Journal of innate immunity 2014 imex curation I think you mean there is a recip. interaction in Fig 2A - only this involves NLRP3 (not NLRC3)- which I could find no origin for - added to comment at Publication level. Again Caspase-1 I could find not origin for.; removed interaction with caspase-1 asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS DIP protein Q9ULZ3 original sequence version: 116 455987286586F46A nlrc3_human NLR family CARD domain-containing protein 3 NLRC3 Nucleotide-binding oligomerization domain protein 3 Caterpiller protein 16.2 CARD15-like protein NOD3 NACHT, LRR and CARD domains-containing protein 3 protein protein human Homo sapiens Human MRKQEVRTGREAGQGHGTGSPAEQVKALMDLLAGKGSQGSQAPQALDRTPDAPLGPCSNDSRIQRHRKALLSKVGGGPELGGPWHRLASLLLVEGLTDLQLREHDFTQVEATRGGGHPARTVALDRLFLPLSRVSVPPRVSITIGVAGMGKTTLVRHFVRLWAHGQVGKDFSLVLPLTFRDLNTHEKLCADRLICSVFPHVGEPSLAVAVPARALLILDGLDECRTPLDFSNTVACTDPKKEIPVDHLITNIIRGNLFPEVSIWITSRPSASGQIPGGLVDRMTEIRGFNEEEIKVCLEQMFPEDQALLGWMLSQVQADRALYLMCTVPAFCRLTGMALGHLWRSRTGPQDAELWPPRTLCELYSWYFRMALSGEGQEKGKASPRIEQVAHGGRKMVGTLGRLAFHGLLKKKYVFYEQDMKAFGVDLALLQGAPCSCFLQREETLASSVAYCFTHLSLQEFVAAAYYYGASRRAIFDLFTESGVSWPRLGFLTHFRSAAQRAMQAEDGRLDVFLRFLSGLLSPRVNALLAGSLLAQGEHQAYRTQVAELLQGCLRPDAAVCARAINVLHCLHELQHTELARSVEEAMESGALARLTGPAHRAALAYLLQVSDACAQEANLSLSLSQGVLQSLLPQLLYCRKLRLDTNQFQDPVMELLGSVLSGKDCRIQKISLAENQISNKGAKALARSLLVNRSLTSLDLRGNSIGPQGAKALADALKINRTLTSLSLQGNTVRDDGARSMAEALASNRTLSMLHLQKNSIGPMGAQRMADALKQNRSLKELMFSSNSIGDGGAKALAEALKVNQGLESLDLQSNSISDAGVAALMGALCTNQTLLSLSLRENSISPEGAQAIAHALCANSTLKNLDLTANLLHDQGARAIAVAVRENRTLTSLHLQWNFIQAGAAQALGQALQLNRSLTSLDLQENAIGDDGACAVARALKVNTALTALYLQVASIGASGAQVLGEALAVNRTLEILDLRGNAIGVAGAKALANALKVNSSLRRLNLQENSLGMDGAICIATALSGNHRLQHINLQGNHIGDSGARMISEAIKTNAPTCTVEM 9BFFCB21FD134562 nlrc3-pycard-1 3 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2A pycard-nlrc3-1 1 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 2B NLRC3 and ASC colocalization is only partial. pycard-nlrc3-2 2 4 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp fig 1