IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ De novo TBR1 mutations in sporadic autism disrupt protein functions. De novo TBR1 mutations in sporadic autism disrupt protein functions. Nature communications 2014 imex curation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Accepted 2018-JUN-05 AT 09:53 BST AT 09:53 BST by PPORRAS Nature communications 2014 Only protein-protein interactions imex curation imex curation I have removed "Supp fig 6B" from EBI-16628317 as this figure is TBR1 interacting with TBR1, so new interaction created for SUpp Fig 6B. EBI-16628317 is TBR1 with FOXP2. I have removed mutations associated with the wrong protein.; altered; EBI-16595119 is currently labelled Supp figure 6B - which is for a different cell line (293), and I have already re-created this in Experiment-3. I have altered the figure legend to Supp 7 and removed the two TBR1 mutations. De novo TBR1 mutations in sporadic autism disrupt protein functions. De novo TBR1 mutations in sporadic autism disrupt protein functions. Nature communications 2014 imex curation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Accepted 2018-JUN-05 AT 09:53 BST AT 09:53 BST by PPORRAS Nature communications 2014 Only protein-protein interactions imex curation imex curation I have removed "Supp fig 6B" from EBI-16628317 as this figure is TBR1 interacting with TBR1, so new interaction created for SUpp Fig 6B. EBI-16628317 is TBR1 with FOXP2. I have removed mutations associated with the wrong protein.; altered; EBI-16595119 is currently labelled Supp figure 6B - which is for a different cell line (293), and I have already re-created this in Experiment-3. I have altered the figure legend to Supp 7 and removed the two TBR1 mutations. De novo TBR1 mutations in sporadic autism disrupt protein functions. De novo TBR1 mutations in sporadic autism disrupt protein functions. Nature communications 2014 imex curation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl 09-May-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. bret bioluminescence resonance energy transfer BRET LRET LRET BRET bret tag visualisation tag visualisation Deriziotis P., O'Roak BJ., Graham SA., Estruch SB., Dimitropoulou D., Bernier RA., Gerdts J., Shendure J., Eichler EE., Fisher SE. Fig 6A gives Ref 19 (Sakai et al) for isoforms of FOXP2 - however NO trace of them there. [Ref 40 states:-Exon 10+ was detected in five independent clones from three different sources of cDNA (Fig. 1B) and encodes ten additional amino acids not present in the published exon 10 (Fig. 3)] Fig 3 gives total length and sequence of C-terminus of isoform 10+.] The isoforms of FOXP2, as given in figure 6A, have been assigned Uniprot identifiers as follows:- Isoform 1 =O15409-1, isoform III as O15409-2, isoform 10+ (FOXP2-S) as O15409-6 (see Ref 40- PMID:12189486). simon.fisher@mpi.nl Accepted 2018-JUN-05 AT 09:53 BST AT 09:53 BST by PPORRAS Nature communications 2014 Only protein-protein interactions imex curation imex curation I have removed "Supp fig 6B" from EBI-16628317 as this figure is TBR1 interacting with TBR1, so new interaction created for SUpp Fig 6B. EBI-16628317 is TBR1 with FOXP2. I have removed mutations associated with the wrong protein.; altered; EBI-16595119 is currently labelled Supp figure 6B - which is for a different cell line (293), and I have already re-created this in Experiment-3. I have altered the figure legend to Supp 7 and removed the two TBR1 mutations. foxp1_human Forkhead box protein P1 FOXP1 Mac-1-regulated forkhead HSPC215 protein protein human Homo sapiens Human MMQESGTETKSNGSAIQNGSGGSNHLLECGGLREGRSNGETPAVDIGAADLAHAQQQQQQALQVARQLLLQQQQQQQVSGLKSPKRNDKQPALQVPVSVAMMTPQVITPQQMQQILQQQVLSPQQLQVLLQQQQALMLQQQQLQEFYKKQQEQLQLQLLQQQHAGKQPKEQQQVATQQLAFQQQLLQMQQLQQQHLLSLQRQGLLTIQPGQPALPLQPLAQGMIPTELQQLWKEVTSAHTAEETTGNNHSSLDLTTTCVSSSAPSKTSLIMNPHASTNGQLSVHTPKRESLSHEEHPHSHPLYGHGVCKWPGCEAVCEDFQSFLKHLNSEHALDDRSTAQCRVQMQVVQQLELQLAKDKERLQAMMTHLHVKSTEPKAAPQPLNLVSSVTLSKSASEASPQSLPHTPTTPTAPLTPVTQGPSVITTTSMHTVGPIRRRYSDKYNVPISSADIAQNQEFYKNAEVRPPFTYASLIRQAILESPEKQLTLNEIYNWFTRMFAYFRRNAATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEVEFQKRRPQKISGNPSLIKNMQSSHAYCTPLNAALQASMAENSIPLYTTASMGNPTLGNLASAIREELNGAMEHTNSNESDSSPGRSPMQAVHPVHVKEEPLDPEEAEGPLSLVTTANHSPDFDHDRDYEDEPVNEDME DIP protein Q9H334 original sequence version: 89 AEE92D47BB20964B foxp4_human Forkhead box protein P4 FOXP4 FKHLA Fork head-related protein-like A protein protein human Homo sapiens Human MMVESASETIRSAPSGQNGVGSLSGQADGSSGGATGTTASGTGREVTTGADSNGEMSPAELLHFQQQQALQVARQFLLQQASGLSSPGNNDSKQSASAVQVPVSVAMMSPQMLTPQQMQQILSPPQLQALLQQQQALMLQQLQEYYKKQQEQLHLQLLTQQQAGKPQPKEALGNKQLAFQQQLLQMQQLQQQHLLNLQRQGLVSLQPNQASGPLQTLPQAAVCPTDLPQLWKGEGAPGQPAEDSVKQEGLDLTGTAATATSFAAPPKVSPPLSHHTLPNGQPTVLTSRRDSSSHEETPGSHPLYGHGECKWPGCETLCEDLGQFIKHLNTEHALDDRSTAQCRVQMQVVQQLEIQLAKESERLQAMMAHLHMRPSEPKPFSQPLNPVPGSSSFSKVTVSAADSFPDGLVHPPTSAAAPVTPLRPPGLGSASLHGGGPARRRSSDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNEIYNWFTRMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMISGLSYGALNASYQAALAESSFPLLNSPGMLNPGSASSLLPLSHDDVGAPVEPLPSNGSSSPPRLSPPQYSHQVQVKEEPAEAEEDRQPGPPLGAPNPSASGPPEDRDLEEELPGEELS DIP protein Q8IVH2 original sequence version: 64 A63826ED85B24752 o15409-2 Forkhead box protein P2 CAG repeat protein 44 Trinucleotide repeat-containing gene 10 protein III IV FOXP2 CAGH44 TNRC10 protein protein human Homo sapiens Human MMTPQVITPQQMQQILQQQVLSPQQLQALLQQQQAVMLQQQQLQEFYKKQQEQLHLQLLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHPGKQAKEQQQQQQQQQQLAAQQLVFQQQLLQMQQLQQQQHLLSLQRQGLISIPPGQAALPVQSLPQAGLSPAEIQQLWKEVTGVHSMEDNGIKHGGLDLTTNNSSSTTSSNTSKASPPITHHSIVNGQSSVLSARRDSSSHEETGASHTLYGHGVCKWPGCESICEDFGQFLKHLNNEHALDDRSTAQCRVQMQVVQQLEIQLSKERERLQAMMTHLHMRPSEPKPSPKPLNLVSSVTMSKNMLETSPQSLPQTPTTPTAPVTPITQGPSVITPASVPNVGAIRRRHSDKYNIPMSSEIAPNYEFYKNADVRPPFTYATLIRQAIMESSDRQLTLNEIYSWFTRTFAYFRRNAATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEVEYQKRRSQKITGSPTLVKNIPTSLGYGAALNASLQAALAESSLPLLSNPGLINNASSGLLQAVHEDLNGSLDHIDSNGNSSPGCSPQPHIHSIHVKEEPVIAEDEDCPMSLVTTANHSPELEDDREIEEEPLSEDLE F78EA67F33A3AE05 o15409-1 Forkhead box protein P2 CAG repeat protein 44 Trinucleotide repeat-containing gene 10 protein I FOXP2 CAGH44 TNRC10 protein protein human Homo sapiens Human MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEVSTVELLHLQQQQALQAARQLLLQQQTSGLKSPKSSDKQRPLQVPVSVAMMTPQVITPQQMQQILQQQVLSPQQLQALLQQQQAVMLQQQQLQEFYKKQQEQLHLQLLQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHPGKQAKEQQQQQQQQQQLAAQQLVFQQQLLQMQQLQQQQHLLSLQRQGLISIPPGQAALPVQSLPQAGLSPAEIQQLWKEVTGVHSMEDNGIKHGGLDLTTNNSSSTTSSNTSKASPPITHHSIVNGQSSVLSARRDSSSHEETGASHTLYGHGVCKWPGCESICEDFGQFLKHLNNEHALDDRSTAQCRVQMQVVQQLEIQLSKERERLQAMMTHLHMRPSEPKPSPKPLNLVSSVTMSKNMLETSPQSLPQTPTTPTAPVTPITQGPSVITPASVPNVGAIRRRHSDKYNIPMSSEIAPNYEFYKNADVRPPFTYATLIRQAIMESSDRQLTLNEIYSWFTRTFAYFRRNAATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEVEYQKRRSQKITGSPTLVKNIPTSLGYGAALNASLQAALAESSLPLLSNPGLINNASSGLLQAVHEDLNGSLDHIDSNGNSSPGCSPQPHIHSIHVKEEPVIAEDEDCPMSLVTTANHSPELEDDREIEEEPLSEDLE 4F9FBDB6D90516E0 tbr1_human T-box brain protein 1 TES-56 TBR1 protein protein human Homo sapiens Human MQLEHCLSPSIMLSKKFLNVSSSYPHSGGSELVLHDHPIISTTDNLERSSPLKKITRGMTNQSDTDNFPDSKDSPGDVQRSKLSPVLDGVSELRHSFDGSAADRYLLSQSSQPQSAATAPSAMFPYPGQHGPAHPAFSIGSPSRYMAHHPVITNGAYNSLLSNSSPQGYPTAGYPYPQQYGHSYQGAPFYQFSSTQPGLVPGKAQVYLCNRPLWLKFHRHQTEMIITKQGRRMFPFLSFNISGLDPTAHYNIFVDVILADPNHWRFQGGKWVPCGKADTNVQGNRVYMHPDSPNTGAHWMRQEISFGKLKLTNNKGASNNNGQMVVLQSLHKYQPRLHVVEVNEDGTEDTSQPGRVQTFTFPETQFIAVTAYQNTDITQLKIDHNPFAKGFRDNYDTIYTGCDMDRLTPSPNDSPRSQIVPGARYAMAGSFLQDQFVSNYAKARFHPGAGAGPGPGTDRSVPHTNGLLSPQQAEDPGAPSPQRWFVTPANNRLDFAASAYDTATDFAGNAATLLSYAAAGVKALPLQAAGCTGRPLGYYADPSGWGARSPPQYCGTKSGSVLPCWPNSAAAAARMAGANPYLGEEAEGLAAERSPLPPGAAEDAKPKDLSDSSWIETPSSIKSIDSSDSGIYEQAKRRRISPADTPVSESSSPLKSEVLAQRDCEKNCAKDISGYYGFYSHS E1C8D84206EFBBB5 234wertyutyjg-1 3 7 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell Q16650:p.Lys228Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 16:58:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys228glu' Tue Jun 30 19:06:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys228Glu' Q16650:p.Asn374His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N H Wed May 02 17:46:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn374his' Tue Jun 30 19:18:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn374His' Q16650:p.Gln178Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q E Wed May 02 20:21:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln178glu' Tue Jun 30 16:33:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln178Glu' Q16650:p.Val356Met mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V M Wed May 02 15:02:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val356met' Tue Jun 30 18:33:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val356Met' Q16650:p.Gln418Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q R Wed May 02 19:14:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln418arg' Tue Jun 30 18:26:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln418Arg' Q16650:p.Pro542Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P R Wed May 02 15:37:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro542arg' Tue Jun 30 18:05:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro542Arg' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 6 B/E/F, 7B The canonical FOXP2 isoform (isoform I) was able to interact with TBR1, as was isoform III, which lacks the first 92 amino acids Isoform 10+, showed a complete loss of interaction with TBR1. Truncated TBR1 proteins with "de novo" mutations- A136PfsX80 and S351X, show no binding, as did the R328X mutation. foxp2-tbr1-1 3 6 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 6B-2 tbr1-1 3 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q16650:p.Lys228Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 14:55:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys228glu' Tue Jun 30 20:17:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys228Glu' Q16650:p.Asn374His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N H Wed May 02 19:07:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn374his' Tue Jun 30 16:03:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn374His' Q16650:p.Gln178Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q E Wed May 02 14:30:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln178glu' Tue Jun 30 15:58:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln178Glu' Q16650:p.Val356Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Wed May 02 17:03:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val356met' Tue Jun 30 18:24:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val356Met' Q16650:p.Gln418Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q R Wed May 02 15:09:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln418arg' Tue Jun 30 19:00:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln418Arg' Q16650:p.Pro542Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P R Wed May 02 17:45:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro542arg' Tue Jun 30 19:21:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro542Arg' proximity proximity close-contact colocalization neighbourhood interaction 5 A/E, Supplementary Fig. 6a/c Truncated proteins with "de novo" mutations- A136PfsX80 and S351X, show no binding to self or wild type. The synthetic mutation S568X showed homodimerization, but the N394X mutant showed reduced homodimerization. foxp2-tbr1-3 1 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O15409-1:p.Arg553His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R H Wed May 02 19:53:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg553his' Tue Jun 30 18:37:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg553His' 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supplementary Fig. 7 The canonical FOXP2 isoform (isoform I) was able to interact with TBR1, as was isoform III, which lacks the first 92 amino acids Isoform 10+, showed a complete loss of interaction with TBR1. I have not added any info about the R328X variant as it looks positive to me - and text is not clear if it shows reduced colocalisation. foxp2-tbr1-4 2 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 6C foxp2-tbr1-6 3 7 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range c_terminal_NLS tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n_terminal_region sufficient to bind sufficient binding region sufficient to bind range range range range 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 6 D All the truncated proteins retained the ability to interact with TBR1 in the BRET assay (Fig. 6d), indicating that the TBR1-binding site lies within the N-terminal 258 residues of FOXP2. The polyglutamine tracts are not required for this interaction. I cannot add any more precise figures for Suff/Req. to bind as NO construct aas are given anywhere. tbr1-2 3 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q16650:p.Lys228Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 17:23:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys228glu' Tue Jun 30 16:48:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys228Glu' Q16650:p.Asn374His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N H Wed May 02 15:02:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn374his' Tue Jun 30 17:08:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn374His' Q16650:p.Gln178Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q E Wed May 02 16:32:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln178glu' Tue Jun 30 18:33:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln178Glu' Q16650:p.Val356Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Wed May 02 19:49:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val356met' Tue Jun 30 19:49:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val356Met' Q16650:p.Gln418Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q R Wed May 02 16:29:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln418arg' Tue Jun 30 18:42:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln418Arg' Q16650:p.Pro542Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P R Wed May 02 15:32:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro542arg' Tue Jun 30 20:04:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro542Arg' 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q16650:p.Lys228Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 17:07:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys228glu' Tue Jun 30 16:32:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys228Glu' Q16650:p.Asn374His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N H Wed May 02 20:06:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn374his' Tue Jun 30 16:03:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn374His' Q16650:p.Gln178Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q E Wed May 02 17:48:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln178glu' Tue Jun 30 16:47:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln178Glu' Q16650:p.Val356Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V M Wed May 02 18:24:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val356met' Tue Jun 30 17:20:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val356Met' Q16650:p.Gln418Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q R Wed May 02 18:32:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln418arg' Tue Jun 30 16:15:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln418Arg' Q16650:p.Pro542Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P R Wed May 02 14:31:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro542arg' Tue Jun 30 19:31:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro542Arg' proximity proximity close-contact colocalization neighbourhood interaction Supplementary Fig. 6a/c Truncated proteins with "de novo" mutations- A136PfsX80 and S351X, show no binding to self or wild type. The synthetic mutation S568X showed increased homodimerization, but the N394X mutant showed reduced homodimerization. foxp2-tbr1-7 1 6 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supplementary Fig. 7 foxp2-tbr1-8 2 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O15409-1:p.Arg553His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:51:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg553his' Tue Jun 30 15:59:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg553His' O15409-1:p.Glu400del mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E . Wed May 02 18:02:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu400del' Tue Jun 30 15:58:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu400del' 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 6C, 7C, Supplementary figure 8f The canonical FOXP2 isoform (isoform I) was able to interact with TBR1, as was isoform III, which lacks the first 92 amino acids Isoform 10+, showed a complete loss of interaction with TBR1. I have not added any info about the R328X variant as it looks positive to me - and text is not clear if it shows reduced colocalisation. foxp2-tbr1-10 3 4 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction Supplementary figure 8B Both FOXP1 and FOXP4 were able to interact with TBR1, both FOXP1 and FOXP4 were able to interact with TBR1, foxp1-tbr1-1 3 5 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction Supplementary figure 8B-2 Both FOXP1 and FOXP4 were able to interact with TBR1, foxp2-tbr1-11 3 7 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell n_terminus renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range O15409-1:p.Glu400del mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E . Wed May 02 14:58:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu400del' Tue Jun 30 16:25:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu400del' 8 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction Supplementary figure 8E foxp2-tbr1-12 2 5 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supplementary Fig. 8C foxp4-tbr1-1 2 4 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supplementary Fig. 8C-2 foxp2-tbr1-13 2 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q16650:p.Asn374His mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N H Fri Dec 08 14:37:05 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'asn374his' Wed May 02 20:13:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp374his' Wed Jun 13 18:50:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp374His' Tue Jun 30 16:23:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn374His' Q16650:p.Lys228Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K E Fri Dec 08 14:46:47 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'lys228glu' Wed May 02 14:28:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln228glu' Wed Jun 13 19:04:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln228Glu' Tue Jun 30 19:51:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys228Glu' 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell n_terminus mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supplementary Fig. 6b