DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262204X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262198X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262206X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262200X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262205X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262199X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262197X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262195X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262202X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262203X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262196X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. Nature structural & molecular biology 2014 imex curation Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Hara K., Zheng G., Qu Q., Liu H., Ouyang Z., Chen Z., Tomchick DR., Yu H. DIP original experiment: DIP-262201X Nature structural & molecular biology 2014 IMEx Structure of cohesin subcomplex pinpoints direct shugoshin-Wapl antagonism in centromeric cohesion. rad21_human Double-strand-break repair protein rad21 homolog RAD21 HR21 KIAA0078 Nuclear matrix protein 1 SCC1 homolog NXP1 SCC1 protein protein human Homo sapiens Human MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRKAKYLLADCNEAFIKIKMAFRPGVVDLPEENREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFSLNQSRVEEITMREEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTTTSNLLLESEQSTSNLNEKINHLEYEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNVSMGGPDSPDSVDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTTLDLAPPTKKLMMWKETGGVEKLFSLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEFENPEVPREDQQQQHQQRDVIDEPIIEEPSRLQESVMEASRTNIDESAMPPPPPQGVKRKAGQIDPEPVMPPQQVEQMEIPPVELPPEEPPNICQLIPELELLPEKEKEKEKEKEDDEEEEDEDASGGDQDQEERRWNKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHII DIP protein O60216 original sequence version: 72 DIP protein O60216 original sequence version: 57 DIP protein O60216 original sequence version: 113 DIP protein O60216 original sequence version: 128 DIP protein O60216 original sequence version: 134 7D4A6EA6392BE73D stag2_human Cohesin subunit SA-2 STAG2 SA2 Stromal antigen 2 SCC3 homolog 2 protein protein human Homo sapiens Human MIAAPEIPTDFNLLQESETHFSSDTDFEDIEGKNQKQGKGKTCKKGKKGPAEKGKGGNGGGKPPSGPNRMNGHHQQNGVENMMLFEVVKMGKSAMQSVVDDWIESYKHDRDIALLDLINFFIQCSGCKGVVTAEMFRHMQNSEIIRKMTEEFDEDSGDYPLTMAGPQWKKFKSSFCEFIGVLVRQCQYSIIYDEYMMDTVISLLTGLSDSQVRAFRHTSTLAAMKLMTALVNVALNLSINMDNTQRQYEAERNKMIGKRANERLELLLQKRKELQENQDEIENMMNAIFKGVFVHRYRDAIAEIRAICIEEIGIWMKMYSDAFLNDSYLKYVGWTMHDKQGEVRLKCLTALQGLYYNKELNSKLELFTSRFKDRIVSMTLDKEYDVAVQAIKLLTLVLQSSEEVLTAEDCENVYHLVYSAHRPVAVAAGEFLYKKLFSRRDPEEDGMMKRRGRQGPNANLVKTLVFFFLESELHEHAAYLVDSMWDCATELLKDWECMNSLLLEEPLSGEEALTDRQESALIEIMLCTIRQAAECHPPVGRGTGKRVLTAKEKKTQLDDRTKITELFAVALPQLLAKYSVDAEKVTNLLQLPQYFDLEIYTTGRLEKHLDALLRQIRNIVEKHTDTDVLEACSKTYHALCNEEFTIFNRVDISRSQLIDELADKFNRLLEDFLQEGEEPDEDDAYQVLSTLKRITAFHNAHDLSKWDLFACNYKLLKTGIENGDMPEQIVIHALQCTHYVILWQLAKITESSSTKEDLLRLKKQMRVFCQICQHYLTNVNTTVKEQAFTILCDILMIFSHQIMSGGRDMLEPLVYTPDSSLQSELLSFILDHVFIEQDDDNNSADGQQEDEASKIEALHKRRNLLAAFCKLIVYTVVEMNTAADIFKQYMKYYNDYGDIIKETMSKTRQIDKIQCAKTLILSLQQLFNEMIQENGYNFDRSSSTFSGIKELARRFALTFGLDQLKTREAIAMLHKDGIEFAFKEPNPQGESHPPLNLAFLDILSEFSSKLLRQDKRTVYVYLEKFMTFQMSLRREDVWLPLMSYRNSLLAGGDDDTMSVISGISSRGSTVRSKKSKPSTGKRKVVEGMQLSLTEESSSSDSMWLSREQTLHTPVMMQTPQLTSTIMREPKRLRPEDSFMSVYPMQTEHHQTPLDYNRRGTSLMEDDEEPIVEDVMMSSEGRIEDLNEGMDFDTMDIDLPPSKNRRERTELKPDFFDPASIMDESVLGVSMF DIP protein Q8N3U4 original sequence version: 61 DIP protein Q8N3U4 original sequence version: 104 DIP protein Q8N3U4 original sequence version: 117 DIP protein Q8N3U4 original sequence version: 123 692358EFFFF61BB9 wapl_human Wings apart-like protein homolog WAPL FOE Friend of EBNA2 protein KIAA0261 WAPAL WAPL cohesin release factor protein protein human Homo sapiens Human MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAENLDFTEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTVRLSSKEPNQKDDGVFKAPAPPSKVIKTVTIPTQPYQDIVTALKCRREDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC DIP protein Q7Z5K2 original sequence version: 86 DIP protein Q7Z5K2 original sequence version: 92 A01026E520BFAB2F sgo1_human Shugoshin 1 SGO1 SGOL1 Serologically defined breast cancer antigen NY-BR-85 Shugoshin-like 1 protein protein human Homo sapiens Human MAKERCLKKSFQDSLEDIKKRMKEKRNKNLAEIGKRRSFIAAPCQIITNTSTLLKNYQDNNKMLVLALENEKSKVKEAQDIILQLRKECYYLTCQLYALKGKLTSQQTVEPAQNQEICSSGMDPNSDDSSRNLFVKDLPQIPLEETELPGQGESFQIEDQIPTIPQDTLGVDFDSGEAKSTDNVLPRTVSVRSSLKKHCNSICQFDSLDDFETSHLAGKSFEFERVGFLDPLVNMHIPENVQHNACQWSKDQVNLSPKLIQPGTFTKTKEDILESKSEQTKSKQRDTQERKREEKRKANRRKSKRMSKYKENKSENKKTVPQKKMHKSVSSNDAYNFNLEEGVHLTPFRQKVSNDSNREENNESEVSLCESSGSGDDSDDLYLPTCKYIQNPTSNSDRPVTRPLAKRALKYTDEKETEGSKPTKTPTTTPPETQQSPHLSLKDITNVSLYPVVKIRRLSLSPKKNKASPAVALPKRRCTASVNYKEPTLASKLRRGDPFTDLCFLNSPIFKQKKDLRRSKKRALEVSPAKEAIFILYYVREFVSRFPDCRKCKLETHICLR DIP protein Q5FBB7 original sequence version: 89 DIP protein Q5FBB7 original sequence version: 64 DIP protein Q5FBB7 original sequence version: 92 DIP protein Q5FBB7 original sequence version: 98 90CEE0FD2B40CD9C rad21-stag2-1 7 13 unspecified role unspecified role bait bait Region 211-420 Region 211-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 14 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8N3U4:p.Asp793Lys D793K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D K This feature has been corrected as a result of our quality control procedures. The original label was 'D793K'. Wed May 02 14:46:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp793lys' Tue Jun 30 19:45:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp793Lys' in vitro In vitro direct interaction direct interaction rad21-stag2-2 8 13 unspecified role unspecified role unspecified role unspecified role Region 281-420 Region 281-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 14 unspecified role unspecified role unspecified role unspecified role Region 80-1060 Region 80-1060 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction wapl-rad21-1 5 15 unspecified role unspecified role bait bait Region 410-590 Region 410-590 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 13 unspecified role unspecified role prey prey Region 281-420 Region 281-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 14 unspecified role unspecified role prey prey Region 80-1060 Region 80-1060 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper association association sgo1-rad21-2 12 16 unspecified role unspecified role bait bait Region 313-353 Region 313-353 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pT346 pT346 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain in vitro In vitro 13 unspecified role unspecified role prey prey Region 281-420 Region 281-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 14 unspecified role unspecified role prey prey Region 80-1060 Region 80-1060 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper association association wapl-rad21-2 3 15 unspecified role unspecified role bait bait Region 410-590 Region 410-590 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 13 unspecified role unspecified role prey prey Region 211-420 Region 211-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 14 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro association association rad21-stag2-3 6 13 unspecified role unspecified role bait bait O60216:p.[Leu385Ala;Leu388Ala;Phe389Ala] L385A/L388A/F389A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L385A/L388A/F389A'. Wed May 02 20:32:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu385ala,leu388ala,phe389ala' Tue Jun 30 19:17:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu385Ala;Leu388Ala;Phe389Ala]' Region 383-393 Region 383-393 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 14 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association sgo1-rad21-3 9 16 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role prey prey Region 317-631 Region 317-631 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 14 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human association association rad21-stag2-4 11 13 unspecified role unspecified role unspecified role unspecified role Region 281-420 Region 281-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 14 unspecified role unspecified role unspecified role unspecified role Region 80-1060 Region 80-1060 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction stag2-wapl-1 1 15 unspecified role unspecified role prey prey human Homo sapiens Human 14 unspecified role unspecified role bait bait Q8N3U4:p.Lys290Glu K290E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K290E'. Wed May 02 16:26:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys290glu' Tue Jun 30 19:19:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys290Glu' Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8N3U4:p.Lys330Glu K330E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K330E'. Wed May 02 18:49:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys330glu' Tue Jun 30 20:24:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys330Glu' Q8N3U4:p.Asp326Lys D326K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D K This feature has been corrected as a result of our quality control procedures. The original label was 'D326K'. Wed May 02 15:15:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp326lys' Tue Jun 30 15:53:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp326Lys' Q8N3U4:p.Trp334Ala W334A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W334A'. Wed May 02 18:38:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp334ala' Tue Jun 30 17:27:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp334Ala' human Homo sapiens Human physical association physical association stag2-rad21-2 2 13 unspecified role unspecified role prey prey O60216:p.Asp327Lys D327K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D K This feature has been corrected as a result of our quality control procedures. The original label was 'D327K'. Wed May 02 19:33:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp327lys' Tue Jun 30 17:50:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp327Lys' 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 14 unspecified role unspecified role bait bait Region 80-1060 Region 80-1060 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction sgo1-rad21-4 10 16 unspecified role unspecified role bait bait Region 313-353 Region 313-353 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pT346 pT346 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain in vitro In vitro 13 unspecified role unspecified role prey prey Region 211-420 Region 211-420 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 14 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro association association stag2-rad21-4 4 13 unspecified role unspecified role prey prey human Homo sapiens Human 14 unspecified role unspecified role bait bait Q8N3U4:p.Asp793Lys D793K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D K This feature has been corrected as a result of our quality control procedures. The original label was 'D793K'. Wed May 02 18:04:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp793lys' Tue Jun 30 16:49:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp793Lys' Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association