IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Essential role of eIF5-mimic protein in animal development is linked to control of ATF4 expression. Essential role of eIF5-mimic protein in animal development is linked to control of ATF4 expression. Nucleic acids research 2013 imex curation Hiraishi H., Oatman J., Haller SL., Blunk L., McGivern B., Morris J., Papadopoulos E., Gutierrez W., Gordon M., Bokhari W., Ikeda Y., Miles D., Fellers J., Asano M., Wagner G., Tazi L., Rothenburg S., Brown SJ., Asano K. Details about eIF2-beta construct were given by the authors upon email request. kasano@ksu.edu Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hiraishi H., Oatman J., Haller SL., Blunk L., McGivern B., Morris J., Papadopoulos E., Gutierrez W., Gordon M., Bokhari W., Ikeda Y., Miles D., Fellers J., Asano M., Wagner G., Tazi L., Rothenburg S., Brown SJ., Asano K. Details about eIF2-beta construct were given by the authors upon email request. kasano@ksu.edu Accepted 2017-FEB-03 AT 11:58 GMT AT 11:58 GMT by ORCHARD Nucleic acids research 2013 Only protein-protein interactions imex curation imex curation Essential role of eIF5-mimic protein in animal development is linked to control of ATF4 expression. Essential role of eIF5-mimic protein in animal development is linked to control of ATF4 expression. Nucleic acids research 2013 imex curation Hiraishi H., Oatman J., Haller SL., Blunk L., McGivern B., Morris J., Papadopoulos E., Gutierrez W., Gordon M., Bokhari W., Ikeda Y., Miles D., Fellers J., Asano M., Wagner G., Tazi L., Rothenburg S., Brown SJ., Asano K. Details about eIF2-beta construct were given by the authors upon email request. kasano@ksu.edu Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Hiraishi H., Oatman J., Haller SL., Blunk L., McGivern B., Morris J., Papadopoulos E., Gutierrez W., Gordon M., Bokhari W., Ikeda Y., Miles D., Fellers J., Asano M., Wagner G., Tazi L., Rothenburg S., Brown SJ., Asano K. Details about eIF2-beta construct were given by the authors upon email request. kasano@ksu.edu Accepted 2017-FEB-03 AT 11:58 GMT AT 11:58 GMT by ORCHARD Nucleic acids research 2013 Only protein-protein interactions imex curation imex curation if2g_yeast Eukaryotic translation initiation factor 2 subunit gamma YER025W GCD11 TIF213 SUI4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLQDQEPSIIINGNLEPVGEPDIVEETEVVAQETQETQDADKPKKKVAFTGLEEDGETEEEKRKREFEEGGGLPEQPLNPDFSKLNPLSAEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCQEPTCPEPDCYRSFKSDKEISPKCQRPGCPGRYKLVRHVSFVDCPGHDILMSTMLSGAAVMDAALLLIAGNESCPQPQTSEHLAAIEIMKLKHVIILQNKVDLMREESALEHQKSILKFIRGTIADGAPIVPISAQLKYNIDAVNEFIVKTIPVPPRDFMISPRLIVIRSFDVNKPGAEIEDLKGGVAGGSILNGVFKLGDEIEIRPGIVTKDDKGKIQCKPIFSNIVSLFAEQNDLKFAVPGGLIGVGTKVDPTLCRADRLVGQVVGAKGHLPNIYTDIEINYFLLRRLLGVKTDGQKQAKVRKLEPNEVLMVNIGSTATGARVVAVKADMARLQLTSPACTEINEKIALSRRIEKHWRLIGWATIKKGTTLEPIA D498AE62BC3E81CD D498AE62BC3E81CD k7tu45_wheat 5MP protein protein wheat Triticum aestivum (Wheat) MSSKEKPTLGGQRIKTRKRNIAAPSDPSSFSDAIVQTYLDNAGDLELVAKSIESSDLNFSRYGDTFFEVVFIGVRTQPGTIKPEEEGERHPYSLIDCAAQREAILPYVLFIQKTLRRRPFLIKSLENVMRKFLQSLEFFEENERQKLAIFTALAFSQKLSGLPPETVFQPLLKDNLVAKGIVLSFITEFFKEYLKENSLDDLIALLKKGKMEDNLLEFFPSGKRTSEALSEHFTKEGLTSLVEYNSKKMFEVKLKEIKSTLTTMINEEAEISEVTEVVKQQVKDAKFPDIEVVRMLWDVLMEAVQWSGKNQQQNSNSALRQVNAWAGLMNAFCTSGKLELELIYKVQTQCYEDAKLMKLFPEIIRSLYDQDVLAEDTILLWFRKGSNQKGRQSFVRALEPFVKWLEEAEEEE 2F1DAC1AB2AD23F3 bzw2_human Basic leucine zipper and W2 domain-containing protein 2 BZW2 HSPC028 MSTP017 protein protein human Homo sapiens Human MNKHQKPVLTGQRFKTRKRDEKEKFEPTVFRDTLVQGLNEAGDDLEAVAKFLDSTGSRLDYRRYADTLFDILVAGSMLAPGGTRIDDGDKTKMTNHCVFSANEDHETIRNYAQVFNKLIRRYKYLEKAFEDEMKKLLLFLKAFSETEQTKLAMLSGILLGNGTLPATILTSLFTDSLVKEGIAASFAVKLFKAWMAEKDANSVTSSLRKANLDKRLLELFPVNRQSVDHFAKYFTDAGLKELSDFLRVQQSLGTRKELQKELQERLSQECPIKEVVLYVKEEMKRNDLPETAVIGLLWTCIMNAVEWNKKEELVAEQALKHLKQYAPLLAVFSSQGQSELILLQKVQEYCYDNIHFMKAFQKIVVLFYKADVLSEEAILKWYKEAHVAKGKSVFLDQMKKFVEWLQNAEEESESEGEEN 7ABFE7A51501E721 eif3g_yeast Eukaryotic translation initiation factor 3 subunit G TIF35 YDR429C D9461.16 Eukaryotic translation initiation factor 3 RNA-binding subunit Translation initiation factor eIF3 p33 subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEVAPEEIIENADGSRSIITYKIEDGVKYKITQKVKEVKVLEKVHKSVAERKNWHKYGSEKGSPAGPSAVTARLGEEVELRLSRNWKQAEEERIQKEKASLTKTGLQCRLCGNDHMTMNCPFKTILSELSALEDPATNEGGVEAASEEKAGQVGGAGSIPGQYVPPSRRAGARDPSSDAYRDSRERDDMCTLKIMQVNENADENSLREELLFPFAPIPRVSVVRNKETGKSRGLAFVTFSSEEVAEQALRFLDGRGYMNLILRVEWSKPKVKE 340A5285F2C84E06 340A5285F2C84E06 t2esu9_9basi 5MP protein protein Puccinia triticina Puccinia triticina MSAAAAAKPSLAGVRNKQRKGVAKAAAKFEPEEFRDQILKHLSTLPPNDLDAVAAKLDTLGNQLDYRKYEGPLFEVLLVGGILAPGGSFVDDGAERSPFSILTSTQYPIDQADMKKHVDVFNKLIRRYKYLQKIFEENALTGLLQYINKFEGPDLEKLAYSLALFMSTALTSSNVLLTLQKEHLVKDGVSLNFITKVFTAYLQVESADHLSGALRRGGVVDMYGFFPPHKREPSDLEAHFKAEKLDAIVAYAHKQRSGQVRDETLVRLREMVASEDPQSEIISFLQEQAQSGLIPDVDFIALVWHGLMDRLDMGSRPDQIVDMVVRAVVAVAPVLAAFCKNARTQISMINTIQVWAYENTKVMGAFAKIIKELYAHDALSEQAILYWHSKGSKAQARQHFLKSTEPLVKFLKEQDEDDDDDE 60AAFB5A90BAAFD0 m1r6y5_giain 5MP DHA2_7522 protein protein giain Giardia intestinalis MSKPLPGALADTKIRTRKRNIVVQKDPESFLEALEHLFVGDSLEEVFKNLDSATDIDYKTYHEFFFDRFISGSIGVCFGRVDKRKTPRSPSIFADSLSKVDAWISILERFMRKRPYMRIGIGEVIGRAMLTIDNIPQYLDHVMSICSHLANLDILKYEKTTEFFLLKLLRINHLVVSGEALSILSKFTNLYATLSSQQKAWEYLIAGEVVDKLFDFCPEHCSDVLKLLEDSYGMQWFVELYRTNQTKNNITTWEQKIIQFMQKDPEGEEETLEALREDALRQVKQCKEECGMSAEESVMFMWRCIVSLSKTMSKDIVGALKKWGPCLYTLVGDDLVMQGKLLSTVQTTCYNNPNIINSFKPIVVQLYDLEILEADVIIAWANGNSGPGRSLFGEQLAEFVTWLEADDGED FD6401DF47DF19EF if2b_human Eukaryotic translation initiation factor 2 subunit 2 Eukaryotic translation initiation factor 2 subunit beta EIF2S2 EIF2B protein protein human Homo sapiens Human MSGDEMIFDPTMSKKKKKKKKPFMLDEEGDTQTEETQPSETKEVEPEPTEDKDLEADEEDTRKKDASDDLDDLNFFNQKKKKKKTKKIFDIDEAEEGVKDLKIESDVQEPTEPEDDLDIMLGNKKKKKKNVKFPDEDEILEKDEALEDEDNKKDDGISFSNQTGPAWAGSERDYTYEELLNRVFNIMREKNPDMVAGEKRKFVMKPPQVVRVGTKKTSFVNFTDICKLLHRQPKHLLAFLLAELGTSGSIDGNNQLVIKGRFQQKQIENVLRRYIKEYVTCHTCRSPDTILQKDTRLYFLQCETCHSRCSVASIKTGFQAVTGKRAQLRAKAN 3E9FD4B48AA61A51 m4md31_trica 5MP protein protein red flour beetle Tribolium castaneum Red flour beetle MSQKVEKPVLSGQRIKTRKRDEKERYDPVGFRDAIISGLDQCNNNFEAISRFLDGAGNKQDYRRYGESLFDILIAGGILVPGGTLLQEGEAPFKTSACIFNSPDEINLETMKSWEQVFIKLMRRYKYLEKIFQDEIKKVLMFVKYFTPAERKKLSVMVYLWISNGSIPFSAVLVLSNSHLVKDHLALDFLIDVFKYWRQDKGVNSLYSAIKKSNIESHLMSFVPDTKQSQVYFRNAFQENGLEEILKLYNDQHQQLAKKELQVMLADSPSENKPQRDIIAELREFAAKENIQEHETVCIIWTTLMDIPEWSKKEELVTDQAVRHLKLYTSLFSAFTQSARSELNLLLKVQEYCCSNMTFMRAFQKIIMLFYKTDVISEQVILKWYKQDYSVKGKMMFVDQMKQFVEWLQNAEEESDSEGSTE 924A92FBC204ECCE 5mp-gcd11-1 1 6 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 5MP 10 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 2B, lane 4 5mp-gcd11-2 1 6 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 5MP 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 2B, lane 5 5mp-gcd11-3 1 6 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey 5MP 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 2B, lane 6 5mp-gcd11-4 1 3 unspecified role unspecified role prey prey 5MP 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2B, lane 7 bzw2-eif2s2-1 2 eIF2-beta 9 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gb1 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5MP1 5 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 5A bzw2-eif2s2-2 2 eIF2-beta 9 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gb1 tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5MP1-CTD 5 unspecified role unspecified role neutral component neutral component purified purified n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range CTD fragment sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 5B