DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Nature 2014 imex curation Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. DIP original experiment: DIP-262320X Nature 2014 IMEx Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Nature 2014 imex curation Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. DIP original experiment: DIP-262324X Nature 2014 IMEx Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Nature 2014 imex curation Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. DIP original experiment: DIP-262323X Nature 2014 IMEx Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Nature 2014 imex curation Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. DIP original experiment: DIP-262321X Nature 2014 IMEx Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. Nature 2014 imex curation Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Wright KE., Hjerrild KA., Bartlett J., Douglas AD., Jin J., Brown RE., Illingworth JJ., Ashfield R., Clemmensen SB., de Jongh WA., Draper SJ., Higgins MK. DIP original experiment: DIP-262322X Nature 2014 IMEx Structure of malaria invasion protein RH5 with erythrocyte basigin and blocking antibodies. p35613-2 Basigin Short BSG 5F7 Collagenase stimulatory factor Extracellular matrix metalloproteinase inducer Leukocyte activation antigen M6 OK blood group antigen Tumor cell-derived collagenase stimulatory factor UNQ6505/PRO21383 Hepatoma-associated antigen Basigin-2 protein protein human Homo sapiens Human MAAALFVLLGFALLGTHGASGAAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALWPFLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS DIP protein P35613-2 original sequence version: 163 0C9D6BEB966AC258 b2l3n7_plafa protein protein plafa Plasmodium falciparum MIRIKKKLILTIIYIHLFILNRLSFENAIKKTKNQENNLTLLPIKSTEEEKDDIKNGKDIKKEIDNDKENIKTNNAKDHSTYIKSYLNTNVNDGLKYLFIPSHNSFIKKYSVFNQINDGMLLNEKNDVKNNEDYKNVDYKNVNFLQYHFKELSNYNIANSIDILQEKEGHLDFVIIPHYTFLDYYKHLSYNSIYHKSSTYGKYIAVDAFIKKINETYDKVKSKCNDIKNDLIATIKKLEHPYDINNKNDDSYRYDISEEIDDKSEETDDETEEVEDSIQDTDSNHTPSNKKKNDLMNRTFKKMMDEYNTKKKKLIKCIKNHENDFNKICMDMKNYGTNLFEQLSCYNNNFCNTNGIRYHYDEYIHKLILSVKSKNLNKDLSDMTNILQQSELLLTNLNKKMGSYIYIDTIKFIHKEMKHIFNRIEYHTKIINDKTKIIQDKIKLNIWRTFQKDELLKRILDMSNEYSLFITSDHLRQMLYNTFYSKEKHLNNIFHHLIYVLQMKFNDVPIKMEYFQTYKKNKPLTQ DIP protein B2L3N7 original sequence version: 6 b2l3n7_plafa-bsg-1 1 6 unspecified role unspecified role unspecified role unspecified role Region 22-205 Region 22-205 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 140-247/297-526 Region 140-247/297-526 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction b2l3n7_plafa-bsg-2 5 6 unspecified role unspecified role unspecified role unspecified role Region 22-205 Region 22-205 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 140-526 Region 140-526 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction bsg-b2l3n7_plafa-1 2 6 unspecified role unspecified role bait bait Region 22-205 Region 22-205 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role prey prey Region 140-247/297-526 Region 140-247/297-526 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction b2l3n7_plafa-bsg-3 4 6 unspecified role unspecified role unspecified role unspecified role Region 22-205 Region 22-205 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction b2l3n7_plafa-bsg-4 3 6 unspecified role unspecified role unspecified role unspecified role Region 22-205 Region 22-205 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 140-526 Region 140-526 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction