DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Carbonic anhydrase generates CO2 and H+ that drive spider silk formation via opposite effects on the terminal domains. Carbonic anhydrase generates CO2 and H+ that drive spider silk formation via opposite effects on the terminal domains. PLoS biology 2014 imex curation Andersson M., Chen G., Otikovs M., Landreh M., Nordling K., Kronqvist N., Westermark P., Jörnvall H., Knight S., Ridderstråle Y., Holm L., Meng Q., Jaudzems K., Chesler M., Johansson J., Rising A. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Andersson M., Chen G., Otikovs M., Landreh M., Nordling K., Kronqvist N., Westermark P., Jörnvall H., Knight S., Ridderstråle Y., Holm L., Meng Q., Jaudzems K., Chesler M., Johansson J., Rising A. DIP original experiment: DIP-262005X PLoS biology 2014 IMEx Carbonic anhydrase generates CO2 and H+ that drive spider silk formation via opposite effects on the terminal domains. Carbonic anhydrase generates CO2 and H+ that drive spider silk formation via opposite effects on the terminal domains. Carbonic anhydrase generates CO2 and H+ that drive spider silk formation via opposite effects on the terminal domains. PLoS biology 2014 imex curation Andersson M., Chen G., Otikovs M., Landreh M., Nordling K., Kronqvist N., Westermark P., Jörnvall H., Knight S., Ridderstråle Y., Holm L., Meng Q., Jaudzems K., Chesler M., Johansson J., Rising A. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Andersson M., Chen G., Otikovs M., Landreh M., Nordling K., Kronqvist N., Westermark P., Jörnvall H., Knight S., Ridderstråle Y., Holm L., Meng Q., Jaudzems K., Chesler M., Johansson J., Rising A. DIP original experiment: DIP-262006X PLoS biology 2014 IMEx Carbonic anhydrase generates CO2 and H+ that drive spider silk formation via opposite effects on the terminal domains. k4mtl7_arave protein protein arave Araneus ventricosus Orbweaver spider Epeira ventricosa MQLPTRLSFFFLVLFIQGFLSQSQPIWTNPNAAMTMTNNLVQCASRSGVLTADQMDDMGMMADSVNSQMQKMGPNPPQHRLRAMNTAMAAEVAEVVATSPPQSYSAVLNTIGACLRESMMQATGSVDNAFTNEVMQLVKMLSADSANEVSTASASGASYATSTSSAVSSSQATGYSTAAGYGNAAGAGAGAAAAVSGGAGSAGGYGEGAGAGVRSGAGASAGAAGGYGRATGAGAGGAAAAGASGYGGSYGAGAGAGAAGAGGASAYGAGIGGGYGAGAADAYGGGASAGAGAGAGAGVSGGYGRAAGAGAAAAAGANGYGGSYDAGVGAGTAGAGAGAGASGGYGGGAGEGAGAAAAAGAGAGGAGGYGGGAGSGAGAVARAGAGGAGGYGSGIGGGYGSGAGAAAGAGAGGAGAYGGGYGTGAGAGARGADSAGAAAGYGGGVGTGTGSSAGYGRGAGAGAGAGAAAGSGAGAAGGYGGGYGAGAGAGAGAGGATGNRAGDAFAQVFSQNVINSGVITSTTVTKNSAQAAASSMVSTAAKSLGLDENTARSMANAMSSYAAAMAKSFRNSDEFIRNMSYQMGRMLSNAGAINESTASAAASSASSTVTETVRTYGPAAIFSGAGAGGAGGYAQGYGAGAGAGAGAGTGAGGAGGYGQGYGAGSGAGAGGAGGYGAGAGAGAGAGGASGYGQGYGDGAGAGAGAAAAAGAAAGARGAGGYGGGAGDGAGAGAGAGAAGGYGQGYGAGEGAGAGAGAGGAGGYGAGAGAGGAGGYGQSYGDGAAAAAGSGAGAGGSGGYGAGAGAGAGAGAGSGAGAAGGYGGGAGAGVGAGGAGGYDQGYGAGAGAGSGAGAGGAGGYGGGAGAGADAGAGGAGGYGGGAGAGAGARAGAGGVGGYGQSYGAGAGAGAGVGAGGAGAGGADGYGQGYGAGAGTGAGDAGGYGGGAGAGASAGAGGYGGGAGAGGVGVYGKGYGSGSGAGAAAAAGAGGATGNRAGDAFAQVFSQNVINSGVITSTTVTKNSAQAAASSMVSTAAKSLGLDENTARSMANAMSSYAAAMAKSFRNSDEFIRNMSYQMGRMLSNAGAINESTASAAASSASSTVTETVRTYGPAAIFSGAGAGGAGGYAQGYGAGAGAGAGAGTGAGGAGGYGQGYGAGSGAGAGGAGGYGAGAGAGAGAGDASGYGQGYGDGAGAGAGAAAAAGAAAGARGAGGYGGGAGAGAGAGAGAAGGYGQGYGAGAGEGAGAGAGAGAVAGAGAAAAAGAGAGAGGAEGYGAGAGAGGAGGYGQSYGDGAAAAAGSGAGAGGSGGYGAGAGAGSGAGAAGGYGGGAGAGVGAGGEGGYDQGYGAGAGAGSGGGAGGAGGYGGGAGAGSGGGAGGAGGYGGGAGAGAGAGAGGAGGYGGGAGAGTGARAGAGGVGGYGQSYGAGASAAAGAGVGAGGAGAGGAGGYGQGYGAGAGIGAGDAGGYGGGAGAGASAGAGGYGGGAGAGAGGVGGYGKGYGAGSGAGAAAAAGAGAGSAGGYGRGDGAGAGGASGYGQGYGAGAAAGAAATAGAGASVAGGYGGGAGAAAGAGAGGYGGGYGAVAGSGAGAAAAASSGAGGAAGYGRGYGAGSGAGAGAGTVAAYGGAGGVATSSSSATASGSRIVTSGGYGYGTSAAAGAGVAAGSYAGAVNRLSSAEAASRVSSNIAAIASGGASALPSVISNIYSGVVASGVSSNEALIQALLELLSALVHVLSSASIGNVSSVGVDSTLNVVQDSVGQYVG DIP protein K4MTL7 original sequence version: 2 k4mtl7_arave-1 1 3 unspecified role unspecified role unspecified role unspecified role Region 1622-1766 Region 1622-1766 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction k4mtl7_arave-2 2 3 unspecified role unspecified role unspecified role unspecified role Region 1622-1766 Region 1622-1766 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction