DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Structure (London, England : 1993) 2014 imex curation Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. DIP original experiment: DIP-261980X Structure (London, England : 1993) 2014 IMEx Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Structure (London, England : 1993) 2014 imex curation Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. Only protein-protein interactions in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. DIP original experiment: DIP-261981X Structure (London, England : 1993) 2014 IMEx Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Structure (London, England : 1993) 2014 imex curation Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly sls static light scattering sls predetermined predetermined participant predetermined Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. DIP original experiment: DIP-261978X Structure (London, England : 1993) 2014 IMEx Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Structure (London, England : 1993) 2014 imex curation Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. DIP original experiment: DIP-261982X Structure (London, England : 1993) 2014 IMEx Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Structure (London, England : 1993) 2014 imex curation Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. DIP original experiment: DIP-261977X Structure (London, England : 1993) 2014 IMEx Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. Structure (London, England : 1993) 2014 imex curation Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liu W., Zhan C., Cheng H., Kumar PR., Bonanno JB., Nathenson SG., Almo SC. DIP original experiment: DIP-261979X Structure (London, England : 1993) 2014 IMEx Mechanistic basis for functional promiscuity in the TNF and TNF receptor superfamilies: structure of the LIGHT:DcR3 assembly. tnf6b_human Tumor necrosis factor receptor superfamily member 6B Decoy receptor for Fas ligand Decoy receptor 3 M68 UNQ186/PRO212 TNFRSF6B DCR3 TR6 protein protein human Homo sapiens Human MRALEGPGLSLLCLVLALPALLPVPAVRGVAETPTYPWRDAETGERLVCAQCPPGTFVQRPCRRDSPTTCGPCPPRHYTQFWNYLERCRYCNVLCGEREEEARACHATHNRACRCRTGFFAHAGFCLEHASCPPGAGVIAPGTPSQNTQCQPCPPGTFSASSSSSEQCQPHRNCTALGLALNVPGSSSHDTLCTSCTGFPLSTRVPGAEECERAVIDFVAFQDISIKRLQRLLQALEAPEGWGPTPRAGRAALQLKLRRRLTELLGAQDGALLVRLLQALRVARMPGLERSVRERFLPVH DIP protein O95407 original sequence version: 84 DIP protein O95407 original sequence version: 114 F90AEE33718449AF o43557-1 Tumor necrosis factor ligand superfamily member 14 TNFSF14 HVEML LIGHT UNQ391/PRO726 Herpes virus entry mediator ligand protein protein human Homo sapiens Human MEESVVRPSVFVVDGQTDIPFTRLGRSHRRQSCSVARVGLGLLLLLMGAGLAVQGWFLLQLHWRLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV DIP protein O43557-1 original sequence version: 109 DIP protein O43557-1 original sequence version: 144 tnfsf14-1 5 8 unspecified role unspecified role unspecified role unspecified role Region 83-240 Region 83-240 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction tnfrsf6b-tnfsf14-1 1 7 unspecified role unspecified role unspecified role unspecified role Region 30-195 Region 30-195 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role unspecified role unspecified role Region 83-240 Region 83-240 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction tnfrsf6b-tnfsf14-2 4 7 unspecified role unspecified role unspecified role unspecified role Region 30-195 Region 30-195 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role unspecified role unspecified role Region 83-240 Region 83-240 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction tnfsf14-2 3 8 unspecified role unspecified role unspecified role unspecified role Region 83-240 Region 83-240 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction tnfrsf6b-tnfsf14-3 2 7 unspecified role unspecified role unspecified role unspecified role Region 30-195 Region 30-195 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role unspecified role unspecified role Region 83-240 Region 83-240 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction tnfsf14-3 6 8 unspecified role unspecified role unspecified role unspecified role Region 83-240 Region 83-240 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction