DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions ecolx Escherichia coli crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265582X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265594X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265584X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265591X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265600X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265598X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265587X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265588X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265605X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265579X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265597X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265593X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265581X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265592X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265585X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265602X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265589X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265601X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265583X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265590X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265580X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265578X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265595X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265596X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265586X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265604X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265606X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265603X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. Nature structural & molecular biology 2014 imex curation Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ni Z., Xu C., Guo X., Hunter GO., Kuznetsova OV., Tempel W., Marcon E., Zhong G., Guo H., Kuo WH., Li J., Young P., Olsen JB., Wan C., Loppnau P., El Bakkouri M., Senisterra GA., He H., Huang H., Sidhu SS., Emili A., Murphy S., Mosley AL., Arrowsmith CH., Min J., Greenblatt JF. DIP original experiment: DIP-265599X Nature structural & molecular biology 2014 IMEx RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation. rpb1_human DNA-directed RNA polymerase II subunit RPB1 POLR2A DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit POLR2 RNA-directed RNA polymerase II subunit RPB1 protein protein human Homo sapiens Human MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPASPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN DIP protein P24928 original sequence version: 108 DIP protein P24928 original sequence version: 147 DIP protein P24928 original sequence version: 158 DIP protein P24928 original sequence version: 157 DIP protein P24928 original sequence version: 179 DIP protein P24928 original sequence version: 181 DIP protein P24928 original sequence version: 161 DIP protein P24928 original sequence version: 175 DIP protein P24928-1 original sequence version: 175 DIP protein P24928 original sequence version: 68 DIP protein P24928 original sequence version: 116 28D6FD25693A6472 rpr1a_human Regulation of nuclear pre-mRNA domain-containing protein 1A RPRD1A p15INK4B-related protein P15RS Cyclin-dependent kinase inhibitor 2B-related protein protein protein human Homo sapiens Human MSAFSEAALEKKLSELSNSQQSVQTLSLWLIHHRKHSRPIVTVWERELRKAKPNRKLTFLYLANDVIQNSKRKGPEFTKDFAPVIVEAFKHVSSETDESCKKHLGRVLSIWEERSVYENDVLEQLKQALYGDKKPRKRTYEQIKVDENENCSSLGSPSEPPQTLDLVRALQDLENAASGDAAVHQRIASLPVEVQEVSLLDKITDKESGERLSKMVEDACMLLADYNGRLAAEIDDRKQLTRMLADFLRCQKEALAEKEHKLEEYKRKLARVSLVRKELRSRIQSLPDLSRLPNVTGSHMHLPFAGDIYSED DIP protein Q96P16 original sequence version: 85 61785281F31CB328 ctd-2 RNAPII peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SPSYSPTSPSYSPTSPSYS q96p16-1 Regulation of nuclear pre-mRNA domain-containing protein 1A RPRD1A P15RS Cyclin-dependent kinase inhibitor 2B-related protein p15INK4B-related protein protein protein human Homo sapiens Human MSAFSEAALEKKLSELSNSQQSVQTLSLWLIHHRKHSRPIVTVWERELRKAKPNRKLTFLYLANDVIQNSKRKGPEFTKDFAPVIVEAFKHVSSETDESCKKHLGRVLSIWEERSVYENDVLEQLKQALYGDKKPRKRTYEQIKVDENENCSSLGSPSEPPQTLDLVRALQDLENAASGDAAVHQRIASLPVEVQEVSLLDKITDKESGERLSKMVEDACMLLADYNGRLAAEIDDRKQLTRMLADFLRCQKEALAEKEHKLEEYKRKLARVSLVRKELRSRIQSLPDLSRLPNVTGSHMHLPFAGDIYSED DIP protein Q96P16-1 original sequence version: 85 rpap2_human Putative RNA polymerase II subunit B1 CTD phosphatase RPAP2 RPAP2 C1orf82 RNA polymerase II-associated protein 2 protein protein human Homo sapiens Human MADFAGPSSAGRKAGAPRCSRKAAGTKQTSTLKQEDASKRKAELEAAVRKKIEFERKALHIVEQLLEENITEEFLMECGRFITPAHYSDVVDERSIVKLCGYPLCQKKLGIVPKQKYKISTKTNKVYDITERKSFCSNFCYQASKFFEAQIPKTPVWVREEERHPDFQLLKEEQSGHSGEEVQLCSKAIKTSDIDNPSHFEKQYESSSSSTHSDSSSDNEQDFVSSILPGNRPNSTNIRPQLHQKSIMKKKAGHKANSKHKDKEQTVVDVTEQLGDCKLDSQEKDATCELPLQKVNTQSSSNSTLPERLKASENSESEYSRSEITLVGISKKSAEHFKRKFAKSNQVSRSVSSSVQVCPEVGKRNLLKVLKETLIEWKTEETLRFLYGQNYASVCLKPEASLVKEELDEDDIISDPDSHFPAWRESQNSLDESLPFRGSGTAIKPLPSYENLKKETEKLNLRIREFYRGRYVLGEETTKSQDSEEHDSTFPLIDSSSQNQIRKRIVLEKLSKVLPGLLVPLQITLGDIYTQLKNLVRTFRLTNRNIIHKPAEWTLIAMVLLSLLTPILGIQKHSQEGMVFTRFLDTLLEELHLKNEDLESLTIIFRTSCLPE DIP protein Q8IXW5 original sequence version: 94 43D6DE7C30BB5C96 rpr1b_human Regulation of nuclear pre-mRNA domain-containing protein 1B RPRD1B C20orf77 CREPT Cell cycle-related and expression-elevated protein in tumor protein protein human Homo sapiens Human MSSFSESALEKKLSELSNSQQSVQTLSLWLIHHRKHAGPIVSVWHRELRKAKSNRKLTFLYLANDVIQNSKRKGPEFTREFESVLVDAFSHVAREADEGCKKPLERLLNIWQERSVYGGEFIQQLKLSMEDSKSPPPKATEEKKSLKRTFQQIQEEEDDDYPGSYSPQDPSAGPLLTEELIKALQDLENAASGDATVRQKIASLPQEVQDVSLLEKITDKEAAERLSKTVDEACLLLAEYNGRLAAELEDRRQLARMLVEYTQNQKDVLSEKEKKLEEYKQKLARVTQVRKELKSHIQSLPDLSLLPNVTGGLAPLPSAGDLFSTD DIP protein Q9NQG5 original sequence version: 101 9057EEF6F3D18215 q5vt52-1 Regulation of nuclear pre-mRNA domain-containing protein 2 RPRD2 KIAA0460 HSPC099 protein protein human Homo sapiens Human MAAGGGGGSSKASSSSASSAGALESSLDRKFQSVTNTMESIQGLSSWCIENKKHHSTIVYHWMKWLRRSAYPHRLNLFYLANDVIQNCKRKNAIIFRESFADVLPEAAALVKDPSVSKSVERIFKIWEDRNVYPEEMIVALREALSTTFKTQKQLKENLNKQPNKQWKKSQTSTNPKAALKSKIVAEFRSQALIEELLLYKRSEDQIELKEKQLSTMRVDVCSTETLKCLKDKTGGKKFSKEFEEASSKLEEFVNGLDKQVKNGPSLTEALENAGIFYEAQYKEVKVVANAYKTFANRVNNLKKKLDQLKSTLPDPEESPVPSPSMDAPSPTGSESPFQGMGGEESQSPTMESEKSATPEPVTDNRDVEDMELSDVEDDGSKIIVEDRKEKPAEKSAVSTSVPTKPTENISKASSCTPVPVTMTATPPLPKPVNTSLSPSPALALPNLANVDLAKISSILSSLTSVMKNTGVSPASRPSPGTPTSPSNLTSGLKTPAPATTTSHNPLANILSKVEITPESILSALSKTQTQSAPALQGLSSLLQSVTGNPVPASEAASQSTSASPANTTVSTIKGRNLPSSAQPFIPKSFNYSPNSSTSEVSSTSASKASIGQSPGLPSTTFKLPSNSLGFTATHNTSPAAPPTEVTICQSSEVSKPKLESESTSPSLEMKIHNFLKGNPGFSGLNLNIPILSSLGSSAPSESHPSDFQRGPTSTSIDNIDGTPVRDERSGTPTQDEMMDKPTSSSVDTMSLLSKIISPGSSTPSSTRSPPPGRDESYPRELSNSVSTYRPFGLGSESPYKQPSDGMERPSSLMDSSQEKFYPDTSFQEDEDYRDFEYSGPPPSAMMNLEKKPAKSILKSSKLSDTTEYQPILSSYSHRAQEFGVKSAFPPSVRALLDSSENCDRLSSSPGLFGAFSVRGNEPGSDRSPSPSKNDSFFTPDSNHNSLSQSTTGHLSLPQKQYPDSPHPVPHRSLFSPQNTLAAPTGHPPTSGVEKVLASTISTTSTIEFKNMLKNASRKPSDDKHFGQAPSKGTPSDGVSLSNLTQPSLTATDQQQQEEHYRIETRVSSSCLDLPDSTEEKGAPIETLGYHSASNRRMSGEPIQTVESIRVPGKGNRGHGREASRVGWFDLSTSGSSFDNGPSSASELASLGGGGSGGLTGFKTAPYKERAPQFQESVGSFRSNSFNSTFEHHLPPSPLEHGTPFQREPVGPSSAPPVPPKDHGGIFSRDAPTHLPSVDLSNPFTKEAALAHAAPPPPPGEHSGIPFPTPPPPPPPGEHSSSGGSGVPFSTPPPPPPPVDHSGVVPFPAPPLAEHGVAGAVAVFPKDHSSLLQGTLAEHFGVLPGPRDHGGPTQRDLNGPGLSRVRESLTLPSHSLEHLGPPHGGGGGGGSNSSSGPPLGPSHRDTISRSGIILRSPRPDFRPREPFLSRDPFHSLKRPRPPFARGPPFFAPKRPFFPPRY DIP protein Q5VT52-1 original sequence version: 93 E08CAF3148F89F67 polr2a-rprd1a-1 27 31 unspecified role unspecified role prey prey human Homo sapiens Human 30 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association rprd1a-1 22 33 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rprd1a-polr2a-1 6 35 unspecified role unspecified role prey prey human Homo sapiens Human 33 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 30 unspecified role unspecified role prey prey human Homo sapiens Human association association rprd1b-1 10 35 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ctd-rprd1b-1 20 32 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 35 unspecified role unspecified role unspecified role unspecified role Region 2-135 Region 2-135 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction polr2a-rpap2-1 9 31 unspecified role unspecified role prey prey human Homo sapiens Human 34 unspecified role unspecified role prey prey human Homo sapiens Human 30 unspecified role unspecified role bait bait human Homo sapiens Human association association rprd1a-2 21 33 unspecified role unspecified role unspecified role unspecified role Region 134-312 Region 134-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rprd1a-rprd1b-1 11 35 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 33 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction ctd-rprd1b-2 4 32 unspecified role unspecified role unspecified role unspecified role pS5/12 pS5/12 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 35 unspecified role unspecified role unspecified role unspecified role Region 2-135 Region 2-135 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rprd1b-2 13 35 unspecified role unspecified role unspecified role unspecified role Region 137-326 Region 137-326 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rprd1b-3 1 35 unspecified role unspecified role unspecified role unspecified role Region 137-326 Region 137-326 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rprd1b-polr2a-4 5 31 unspecified role unspecified role prey prey human Homo sapiens Human 30 unspecified role unspecified role prey prey human Homo sapiens Human 35 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human association association ctd-rprd2-1 14 32 unspecified role unspecified role unspecified role unspecified role pS5/12 pS5/12 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm pS8/15 pS8/15 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm pS3/10/17 pS3/10/17 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm chemical synthesis Chemical synthesis (Chemical synthesis) 36 unspecified role unspecified role unspecified role unspecified role Region 15-161 Region 15-161 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rprd1a-3 19 33 unspecified role unspecified role unspecified role unspecified role Region 134-312 Region 134-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ctd-rprd1a-1 12 33 unspecified role unspecified role unspecified role unspecified role Region 2-137 Region 2-137 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96P16-1:p.Arg114Ala R114A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R114A'. Wed May 02 15:51:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg114ala' Tue Jun 30 19:21:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg114Ala' Q96P16-1:p.Asp65Ala D65A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D65A'. Wed May 02 18:47:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp65ala' Tue Jun 30 16:28:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp65Ala' Q96P16-1:p.Arg106Ala R106A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R106A'. Wed May 02 19:16:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg106ala' Tue Jun 30 15:56:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg106Ala' ecolx Escherichia coli 32 unspecified role unspecified role unspecified role unspecified role pS5/12 pS5/12 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm pS8/15 pS8/15 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm pS3/10/17 pS3/10/17 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction rprd1b-4 3 35 unspecified role unspecified role unspecified role unspecified role Region 137-326 Region 137-326 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rprd1a-rprd1b-2 29 35 unspecified role unspecified role prey prey human Homo sapiens Human 33 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association rpap2-rprd1a-1 15 34 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 33 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rprd1a-rpap2-1 16 34 unspecified role unspecified role prey prey human Homo sapiens Human 33 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association rpap2-rprd1b-1 25 35 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 34 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rprd1a-rprd1b-3 7 35 unspecified role unspecified role prey prey Region 171-304 Region 171-304 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 33 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction rprd1b-rprd1a-3 18 31 unspecified role unspecified role prey prey human Homo sapiens Human 35 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association ctd-rprd1b-3 2 35 unspecified role unspecified role unspecified role unspecified role Region 2-135 Region 2-135 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9NQG5:p.Arg114Ala R114A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R114A'. Wed May 02 20:17:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg114ala' Tue Jun 30 16:20:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg114Ala' ecolx Escherichia coli 32 unspecified role unspecified role unspecified role unspecified role pS5/12 pS5/12 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm pS8/15 pS8/15 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm pS3/10/17 pS3/10/17 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain increasing-ptm ptm increasing an interaction increasing-ptm chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction rpap2-rprd1a-2 26 35 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 34 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 33 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli association association rprd1b-polr2a-5 23 30 unspecified role unspecified role prey prey human Homo sapiens Human 35 unspecified role unspecified role bait bait Q9NQG5:p.Arg114Ala R114A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R114A'. Wed May 02 17:07:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg114ala' Tue Jun 30 19:55:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg114Ala' Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association rprd1b-5 8 35 unspecified role unspecified role unspecified role unspecified role Region 171-304 Region 171-304 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ctd-rprd1a-2 17 32 unspecified role unspecified role unspecified role unspecified role pS3/10/17 pS3/10/17 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 33 unspecified role unspecified role unspecified role unspecified role Region 2-137 Region 2-137 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rprd1b-rpap2-1 28 34 unspecified role unspecified role prey prey human Homo sapiens Human 35 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association polr2a-rprd1b-1 24 30 unspecified role unspecified role bait bait human Homo sapiens Human 35 unspecified role unspecified role prey prey Q9NQG5:p.Arg114Ala R114A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R114A'. Wed May 02 20:32:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg114ala' Tue Jun 30 20:14:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg114Ala' Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association