DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265552X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265564X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265551X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265547X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265563X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265575X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265558X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265562X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265576X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265577X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265556X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265569X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265565X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265554X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265560X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265570X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265572X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265557X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265568X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265555X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265548X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265550X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265559X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265571X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265574X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265561X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265567X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265566X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265553X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265549X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. Nature structural & molecular biology 2014 imex curation Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Park SY., Park JE., Kim TS., Kim JH., Kwak MJ., Ku B., Tian L., Murugan RN., Ahn M., Komiya S., Hojo H., Kim NH., Kim BY., Bang JK., Erikson RL., Lee KW., Kim SJ., Oh BH., Yang W., Lee KS. DIP original experiment: DIP-265573X Nature structural & molecular biology 2014 IMEx Molecular basis for unidirectional scaffold switching of human Plk4 in centriole biogenesis. o94986-3 Centrosomal protein of 152 kDa CEP152 KIAA0912 protein protein human Homo sapiens Human MSLDFGSVALPVQNEDEEYDEEDYEREKELQQLLTDLPHDMLDDDLSSPELQYSDCSEDGTDGQPHHPEQLEMSWNEQMLPKSQSVNGYNEIQSLYAGEKCGNVWEENRSKTEDRHPVYHPEEGGDEGGSGYSPPSKCEQTDLYHLPENFRPYTNGQKQEFNNQATNVIKFSDPQWNHFQGPSCQGLEPYNKVTYKPYQSSAQNNGSPAQEITGSDTFEGLQQQFLGANENSAENMQIIQLQVLNKAKERQLENLIEKLNESERQIRYLNHQLVIIKDEKDGLTLSLRESQKLFQNGKEREIQLEAQIKALETQIQALKVNEEQMIKKSRTTEMALESLKQQLVDLHHSESLQRAREQHESIVMGLTKKYEEQVLSLQKNLDATVTALKEQEDICSRLKDHVKQLERNQEAIKLEKTEIINKLTRSLEESQKQCAHLLQSGSVQEVAQLQFQLQQAQKAHAMSANMNKALQEELTELKDEISLYESAAKLGIHPSDSEGELNIELTESYVDLGIKKVNWKKSKVTSIVQEEDPNEELSKDEFILKLKAEVQRLLGSNSMKRHLVSQLQNDLKDCHKKIEDLHQVKKDEKSIEVETKTDTSEKPKNQLWPESSTSDVVRDDILLLKNEIQVLQQQNQELKETEGKLRNTNQDLCNQMRQMVQDFDHDKQEAVDRCERTYQQHHEAMKTQIRESLLAKHALEKQQLFEAYERTHLQLRSELDKLNKEVTAVQECYLEVCREKDNLELTLRKTTEKEQQTQEKIKEKLIQQLEKEWQSKLDQTIKAMKKKTLDCGSQTDQVTTSDVISKKEMAIMIEEQKCTIQQNLEQEKDIAIKGAMKKLEIELELKHCENITKQVEIAVQNAHQRWLGELPELAEYQALVKAEQKKWEEQHEVSVNKRISFAVSEAKEKWKSELENMRKNILPGKELEEKIHSLQKELELKNEEVPVVIRAELAKARSEWNKEKQEEIHRIQEQNEQDYRQFLDDHRNKINEVLAAAKEDFMKQKTELLLQKETELQTCLDQSRREWTMQEAKRIQLEIYQYEEDILTVLGVLLSDTQKEHISDSEDKQLLEIMSTCSSKWMSVQYFEKLKGCIQKAFQDTLPLLVENADPEWKKRNMAELSKDSASQGTGQGDPGPAAGHHAQPLALQATEAEAEENNKVVEELIEENNDMKNKLEELQTLCKTPPRSLSAGAIENACLPCSGGALEELRGQYIKAVKKIKCDMLRYIQESKERAAEMVKAEVLRERQETARKMRKYYLICLQQILQDDGKEGAEKKIMNAASKLATMAKLLETPISSKSQSKTTQSALPLTSEMLIAVKKSKRNDVNQKIPCCIESKSNSVNTITRTLCEQAPKRRAACNLQRLLENSEHQSIKHVGSKETHLEFQFGDGSCKHLNSLPRNVSPEFVPCEGEGGFGLHKKKDLLSDNGSESLPHSAAYPFLGTLGNKPSPRCTPGPSESGCMHITFRDSNERLGLKVYKCNPLMESENAASEKSQGLDVQEPPVKDGGDLSDCLGWPSSSATLSFDSREASFVHGRPQGTLEIPSESVKSKQFSPSGYLSDTEESNMICQTMKCQRYQTPYLSEETTYLEPGKISVNCGHPSRHKADRLKSDFKKLSSTLPSSVCQQPSRKLIVPLSSQQDSGFDSPFVNLD DIP protein O94986-3 original sequence version: 71 DIP protein O94986-3 original sequence version: 120 DIP protein O94986-3 original sequence version: 107 No experimental confirmation available. ce152_human Centrosomal protein of 152 kDa CEP152 KIAA0912 protein protein human Homo sapiens Human MSLDFGSVALPVQNEDEEYDEEDYEREKELQQLLTDLPHDMLDDDLSSPELQYSDCSEDGTDGQPHHPEQLEMSWNEQMLPKSQSVNGYNEIQSLYAGEKCGNVWEENRSKTEDRHPVYHPEEGGDEGGSGYSPPSKCEQTDLYHLPENFRPYTNGQKQEFNNQATNVIKFSDPQWNHFQGPSCQGLEPYNKVTYKPYQSSAQNNGSPAQEITGSDTFEGLQQQFLGANENSAENMQIIQLQVLNKAKERQLENLIEKLNESERQIRYLNHQLVIIKDEKDGLTLSLRESQKLFQNGKEREIQLEAQIKALETQIQALKVNEEQMIKKSRTTEMALESLKQQLVDLHHSESLQRAREQHESIVMGLTKKYEEQVLSLQKNLDATVTALKEQEDICSRLKDHVKQLERNQEAIKLEKTEIINKLTRSLEESQKQCAHLLQSGSVQEVAQLQFQLQQAQKAHAMSANMNKALQEELTELKDEISLYESAAKLGIHPSDSEGELNIELTESYVDLGIKKVNWKKSKVTSIVQEEDPNEELSKDEFILKLKAEVQRLLGSNSMKRHLVSQLQNDLKDCHKKIEDLHQVKKDEKSIEVETKTDTSEKPKNQLWPESSTSDVVRDDILLLKNEIQVLQQQNQELKETEGKLRNTNQDLCNQMRQMVQDFDHDKQEAVDRCERTYQQHHEAMKTQIRESLLAKHALEKQQLFEAYERTHLQLRSELDKLNKEVTAVQECYLEVCREKDNLELTLRKTTEKEQQTQEKIKEKLIQQLEKEWQSKLDQTIKAMKKKTLDCGSQTDQVTTSDVISKKEMAIMIEEQKCTIQQNLEQEKDIAIKGAMKKLEIELELKHCENITKQVEIAVQNAHQRWLGELPELAEYQALVKAEQKKWEEQHEVSVNKRISFAVSEAKEKWKSELENMRKNILPGKELEEKIHSLQKELELKNEEVPVVIRAELAKARSEWNKEKQEEIHRIQEQNEQDYRQFLDDHRNKINEVLAAAKEDFMKQKTELLLQKETELQTCLDQSRREWTMQEAKRIQLEIYQYEEDILTVLGVLLSDTQKEHISDSEDKQLLEIMSTCSSKWMSVQYFEKLKGCIQKAFQDTLPLLVENADPEWKKRNMAELSKDSASQGTGQGDPGPAAGHHAQPLALQATEAEADKKKVLEIKDLCCGHCFQELEKAKQECQDLKGKLEKCCRHLQHLERKHKAVVEKIGEENNKVVEELIEENNDMKNKLEELQTLCKTPPRSLSAGAIENACLPCSGGALEELRGQYIKAVKKIKCDMLRYIQESKERAAEMVKAEVLRERQETARKMRKYYLICLQQILQDDGKEGAEKKIMNAASKLATMAKLLETPISSKSQSKTTQSALPLTSEMLIAVKKSKRNDVNQKIPCCIESKSNSVNTITRTLCEQAPKRRAACNLQRLLENSEHQSIKHVGSKETHLEFQFGDGSCKHLNSLPRNVSPEFVPCEGEGGFGLHKKKDLLSDNGSESLPHSAAYPFLGTLGNKPSPRCTPGPSESGCMHITFRDSNERLGLKVYKCNPLMESENAASEKSQGLDVQEPPVKDGGDLSDCLGWPSSSATLSFDSREASFVHGRPQGTLEIPSESVKSKQFSPSGYLSDTEESNMICQTMKCQRYQTPYLSEETTYLEPGKISVNCGHPSRHKADRLKSDFKKLSSTLPSSVCQQPSRKLIVPLSSQQDSGFDSPFVNLD DIP protein O94986 original sequence version: 120 DIP protein O94986 original sequence version: 107 1727BDA921E1F6BD ce192_human Centrosomal protein of 192 kDa CEP192 KIAA1569 PP8407 protein protein human Homo sapiens Human MEDFRGIAEESFPSFLTNSLFGNSGILENVTLSSNLGLPVAVSTLARDRSSTDNRYPDIQASYLVEGRFSVPSGSSPGSQSDAEPRERLQLSFQDDDSISRKKSYVESQRLSNALSKQSALQMETAGPEEEPAGATESLQGQDLFNRASPLEQAQDSPIDFHLQSWMNNKEPKIVVLDAGKHFEDKTLKSDLSHTSLLENEKLILPTSLEDSSDDDIDDEMFYDDHLEAYFEQLAIPGMIYEDLEGPEPPEKGFKLPTNGLRQANENGSLNCKFQSENNSSLISLDSHSSETTHKESEESQVICLPGTSNSIGTGDSRRYTDGMLPFSSGTWGTEKEIENLKGIVPDLNSECASKDVLVKTLRAIDVKLNSDNFHDANANRGGFDLTDPVKQGAECPHQNKTVLHMDGCLDTETPTVSIQENVDVASLKPISDSGINFTDAIWSPTCERRTCECHESIEKNKDKTDLPQSVVYQNEEGRWVTDLAYYTSFNSKQNLNVSLSDEMNEDFRSGSEAFDLIAQDEEEFNKEHQFIQEENIDAHNTSVALGDTSWGATINYSLLRKSRSTSDLDKDDASYLRLSLGEFFAQRSEALGCLGGGNNVKRPSFGYFIRSPEKREPIALIRKSDVSRGNLEKEMAHLNHDLYSGDLNEQSQAQLSEGSITLQVEAVESTSQVDENDVTLTADKGKTEDTFFMSNKPQRYKDKLPDSGDSMLRISTIASAIAEASVNTDPSQLAAMIKALSNKTRDKTFQEDEKQKDYSHVRHFLPNDLEKSNGSNALDMEKYLKKTEVSRYESALENFSRASMSDTWDLSLPKEQTTQDIHPVDLSATSVSVRAPEENTAAIVYVENGESENQESFRTINSSNSVTNRENNSAVVDVKTCSIDNKLQDVGNDEKATSISTPSDSYSSVRNPRITSLCLLKDCEEIRDNRENQRQNECVSEISNSEKHVTFENHRIVSPKNSDLKNTSPEHGGRGSEDEQESFRPSTSPLSHSSPSEISGTSSSGCALESFGSAAQQQQPPCEQELSPLVCSPAGVSRLTYVSEPESSYPTTATDDALEDRKSDITSELSTTIIQGSPAALEERAMEKLREKVPFQNRGKGTLSSIIQNNSDTRKATETTSLSSKPEYVKPDFRWSKDPSSKSGNLLETSEVGWTSNPEELDPIRLALLGKSGLSCQVGSATSHPVSCQEPIDEDQRISPKDKSTAGREFSGQVSHQTTSENQCTPIPSSTVHSSVADMQNMPAAVHALLTQPSLSAAPFAQRYLGTLPSTGSTTLPQCHAGNATVCGFSGGLPYPAVAGEPVQNSVAVGICLGSNIGSGWMGTSSLCNPYSNTLNQNLLSTTKPFPVPSVGTNCGIEPWDSGVTSGLGSVRVPEELKLPHACCVGIASQTLLSVLNPTDRWLQVSIGVLSISVNGEKVDLSTYRCLVFKNKAIIRPHATEEIKVLFIPSSPGVFRCTFSVASWPCSTDAETIVQAEALASTVTLTAIAESPVIEVETEKKDVLDFGDLTYGGWKALPLKLINRTHATVPIRLIINANAVAWRCFTFSKESVRAPVEVAPCADVVTRLAGPSVVNHMMPASYDGQDPEFLMIWVLFHSPKKQISSSDILDSAEEFSAKVDIEVDSPNPTPVLRSVSLRARAGIARIHAPRDLQTMHFLAKVASSRKQHLPLKNAGNIEVYLDIKVPEQGSHFSVDPKNLLLKPGEEHEVIVSFTPKDPEACEERILKIFVQPFGPQYEVVLKGEVISSGSKPLSPGPCLDIPSILSNKQFLAWGGVPLGRTQLQKLALRNNSASTTQHLRLLIRGQDQDCFQLQNTFGSEQRLTSNCEIRIHPKEDIFISVLFAPTRLSCMLARLEIKQLGNRSQPGIKFTIPLSGYGGTSNLILEGVKKLSDSYMVTVNGLVPGKESKIVFSVRNTGSRAAFVKAVGFKDSQKKVLLDPKVLRIFPDKFVLKERTQENVTLIYNPSDRGINNKTATELSTVYLFGGDEISRQQYRRALLHKPEMIKQILPEHSVLQNINFVEAFQDELLVTEVYDLPQRPNDVQLFYGSMCKIILSVIGEFRDCISSREFLQPSSKASLESTSDLGASGKHGGNVSLDVLPVKGPQGSPLLSRAARPPLDQLASEEPWTVLPEHLILVAPSPCDMAKTGRFQIVNNSVRLLRFELCWPAHCLTVTPQHGCVAPESKLQILVSPNSSLSTKQSMFPWSGLIYIHCDDGQKKIVKVQIREDLTQVELLTRLTSKPFGILSPVSEPSVSHLVKPMTKPPSTKVEIRNKSITFPTTEPGETSESCLELENHGTTDVKWHLSSLAPPYVKGVDESGDVFRATYAAFRCSPISGLLESHGIQKVSITFLPRGRGDYAQFWDVECHPLKEPHMKHTLRFQLSGQSIEAENEPENACLSTDSLIKIDHLVKPRRQAVSEASARIPEQLDVTARGVYAPEDVYRFRPTSVGESRTLKVNLRNNSFITHSLKFLSPREPFYVKHSKYSLRAQHYINMPVQFKPKSAGKFEALLVIQTDEGKSIAIRLIGEALGKN DIP protein Q8TEP8 original sequence version: 90 CA0A92F264280C3B q8tep8-3 Centrosomal protein of 192 kDa CEP192 KIAA1569 PP8407 protein protein human Homo sapiens Human MEDFRGIAEESFPSFLTNSLFGNSGILENVTLSSNLGLPVAVSTLARDRSSTDNRYPDIQASYLVEGRFSVPSGSSPGSQSDAEPRERLQLSFQDDDSISRKKSYVESQRLSNALSKQSALQMETAGPEEEPAGATESLQGQDLFNRASPLEQAQDSPIDFHLQSWMNNKEPKIVVLDAGKHFEDKTLKSDLSHTSLLENEKLILPTSLEDSSDDDIDDEMFYDDHLEAYFEQLAIPGMIYEDLEGPEPPEKGFKLPTNGLRQANENGSLNCKFQSENNSSLISLDSHSSETTHKESEESQVICLPGTSNSIGTGDSRRYTDGMLPFSSGTWGTEKEIENLKGIVPDLNSECASKDVLVKTLRAIDVKLNSDNFHDANANRGGFDLTDPVKQGAECPHQNKTVLHMDGCLDTETPTVSIQENVDVASLKPISDSGINFTDAIWSPTCERRTCECHESIEKNKDKTDLPQSVVYQNEEGRWVTDLAYYTSFNSKQNLNVSLSDEMNEDFRSGSEAFDLIAQDEEEFNKEHQFIQEENIDAHNTSVALGDTSWGATINYSLLRKSRSTSDLDKDDASYLRLSLGEFFAQRSEALGCLGGGNNVKRPSFGYFIRSPEKREPIALIRKSDVSRGNLEKEMAHLNHDLYSGDLNEQSQAQLSEGSITLQVEAVESTSQVDENDVTLTADKGKTEDTFFMSNKPQRYKDKLPDSGDSMLRISTIASAIAEASVNTDPSQLAAMIKALSNKTRDKTFQEDEKQKDYSHVRHFLPNDLEKSNGSNALDMEKYLKKTEVSRYESALENFSRASMSDTWDLSLPKEQTTQDIHPVDLSATSVSVRAPEENTAAIVYVENGESENQESFRTINSSNSVTNRENNSAVVDVKTCSIDNKLQDVGNDEKATSISTPSDSYSSVRNPRITSLCLLKDCEEIRDNRENQRQNECVSEISNSEKHVTFENHRIVSPKNSDLKNTSPEHGGRGSEDEQESFRPSTSPLSHSSPSEISGTSSSGCALESFGSAAQQQQPPCEQELSPLVCSPAGVSRLTYVSEPESSYPTTATDDALEDRKSDITSELSTTIIQGSPAALEERAMEKLREKVPFQNRGKGTLSSIIQNNSDTRKATETTSLSSKPEYVKPDFRWSKDPSSKSGNLLETSEVGWTSNPEELDPIRLALLGKSGLSCQVGSATSHPVSCQEPIDEDQRISPKDKSTAGREFSGQVSHQTTSENQCTPIPSSTVHSSVADMQNMPAAVHALLTQPSLSAAPFAQRYLGTLPSTGSTTLPQCHAGNATVCGFSGGLPYPAVAGEPVQNSVAVGICLGSNIGSGWMGTSSLCNPYSNTLNQNLLSTTKPFPVPSVGTNCGIEPWDSGVTSGLGSVRVPEELKLPHACCVGIASQTLLSVLNPTDRWLQVSIGVLSISVNGEKVDLSTYRCLVFKNKAIIRPHATEEIKVLFIPSSPGVFRCTFSVASWPCSTDAETIVQAEALASTVTLTAIAESPVIEVETEKKDVLDFGDLTYGGWKALPLKLINRTHATVPIRLIINANAVAWRCFTFSKESVRAPVEVAPCADVVTRLAGPSVVNHMMPASYDGQDPEFLMIWVLFHSPKKQISSSDILDSAEEFSAKVDIEVDSPNPTPVLRSVSLRARAGIARIHAPRDLQTMHFLAKVASSRKQHLPLKNAGNIEVYLDIKVPEQGSHFSVDPKNLLLKPGEEHEVIVSFTPKDPEACEERILKIFVQPFGPQYEVVLKGEVISSGSKPLSPGPCLDIPSILSNKQFLAWGGVPLGRTQLQKLALRNNSASTTQHLRLLIRGQDQDCFQLQNTFGSEQRLTSNCEIRIHPKEDIFISVLFAPTRLSCMLARLEIKQLGNRSQPGIKFTIPLSGYGGTSNLILEGVKKLSDSYMVTVNGLVPGKESKIVFSVRNTGSRAAFVKAVGFKDSQKKVLLDPKVLRIFPDKFVLKERTQENVTLIYNPSDRGINNKTATELSTVYLFGGDEISRQQYRRALLHKPEMIKQILPEHSVLQNINFVEAFQDELLVTEVYDLPQRPNDVQLFYGSMCKIILSVIGEFRDCISSREFLQPSSKASLESTSDLGASGKHGGNVSLDVLPVKGPQGSPLLSRAARPPLDQLASEEPWTVLPEHLILVAPSPCDMAKTGRFQIVNNSVRLLRFELCWPAHCLTVTPQHGCVAPESKLQILVSPNSSLSTKQSMFPWSGLIYIHCDDGQKKIVKVQIREDLTQVELLTRLTSKPFGILSPVSEPSVSHLVKPMTKPPSTKVEIRNKSITFPTTEPGETSESCLELENHGTTDVKWHLSSLAPPYVKGVDESGDVFRATYAAFRCSPISGLLESHGIQKVSITFLPRGRGDYAQFWDVECHPLKEPHMKHTLRFQLSGQSIEAENEPENACLSTDSLIKIDHLVKPRRQAVSEASARIPEQLDVTARGVYAPEDVYRFRPTSVGESRTLKVNLRNNSFITHSLKFLSPREPFYVKHSKYSLRAQHYINMPVQFKPKSAGKFEALLVIQTDEGKSIAIRLIGEALGKN DIP protein Q8TEP8-3 original sequence version: 90 Ref.1 (AIA61642) sequence is in conflict in position: 357:V->I. plk4_human Serine/threonine-protein kinase PLK4 Polo-like kinase 4 Serine/threonine-protein kinase Sak Serine/threonine-protein kinase 18 PLK4 SAK STK18 protein protein human Homo sapiens Human MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVEDSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERYSPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLRKTTEYDSISPNRDFQGHPDLQKDTSKNAWTDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQECVFGSDPLSEQSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQEYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKSPKITYFTRYAKCILMENSPGADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMDHANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMHSAASPTQAPILNPSMVTNEGLGLTTTASGTDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFNDGSQLVVQAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNFH DIP protein O00444 original sequence version: 88 DIP protein O00444-1 original sequence version: 93 DIP protein O00444 original sequence version: 145 DIP protein O00444-1 original sequence version: 145 DIP protein O00444 original sequence version: 132 DIP protein O00444-1 original sequence version: 132 DIP protein O00444 original sequence version: 135 4D56F5FD983211A6 cp135_human Centrosomal protein of 135 kDa Centrosomal protein 4 CEP135 CEP4 KIAA0635 protein protein human Homo sapiens Human MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLIAHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV DIP protein Q66GS9 original sequence version: 101 AD56754BEBB3AA5B cep192-plk4-2 4 36 unspecified role unspecified role prey prey human Homo sapiens Human 34 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association cep152-plk4-5 11 32 unspecified role unspecified role unspecified role unspecified role Region 1-60 Region 1-60 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 36 unspecified role unspecified role unspecified role unspecified role Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cep192-plk4-3 16 36 unspecified role unspecified role unspecified role unspecified role Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 35 unspecified role unspecified role unspecified role unspecified role Region 201-258 Region 201-258 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction plk4-cep152-6 2 36 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 32 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human association association cep152-plk4-6 21 33 unspecified role unspecified role bait bait human Homo sapiens Human 36 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association cep192-plk4-4 12 35 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 201-258 Region 201-258 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 36 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association plk4-plk4-7 18 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 36 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association plk4-cep192-1 5 36 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 35 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human association association plk4-plk4-9 30 36 unspecified role unspecified role prey prey Region 698-808 Region 698-808 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 36 unspecified role unspecified role bait bait Region 698-808 Region 698-808 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association cep152-plk4-7 13 32 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-60 Region 1-60 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O94986-3:p.Glu21Lys E21K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E21K'. Wed May 02 18:57:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu21lys' Tue Jun 30 20:11:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu21Lys' O94986-3:p.Val8Ala V8A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V8A'. Wed May 02 19:42:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val8ala' Tue Jun 30 18:28:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val8Ala' human Homo sapiens Human 36 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association cep135-cep152-1 9 32 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 36 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 37 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human association association cep192-plk4-5 22 36 unspecified role unspecified role prey prey Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 35 unspecified role unspecified role bait bait Region 201-258 Region 201-258 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction cep152-plk4-8 24 32 unspecified role unspecified role unspecified role unspecified role Region 1-60 Region 1-60 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 36 unspecified role unspecified role unspecified role unspecified role Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cep135-cep152-2 10 32 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 37 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association plk4-plk4-10 7 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 854-970 Region 854-970 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 36 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 854-970 Region 854-970 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human physical association physical association cep152-plk4-9 3 32 unspecified role unspecified role bait bait Region 1-60 Region 1-60 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 36 unspecified role unspecified role prey prey Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction plk4-cep192-2 23 35 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association plk4-cep192-3 31 36 unspecified role unspecified role bait bait Region 580-970 Region 580-970 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 35 unspecified role unspecified role prey prey Region 201-280 Region 201-280 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction plk4-cep152-7 27 32 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 1-217 Region 1-217 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O00444:p.Lys685Ala K685A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K685A'. Wed May 02 15:44:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys685ala' Tue Jun 30 17:11:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys685Ala' O00444:p.Lys711Ala K711A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K711A'. Wed May 02 19:56:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys711ala' Tue Jun 30 17:16:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys711Ala' human Homo sapiens Human physical association physical association plk4-1 1 36 unspecified role unspecified role unspecified role unspecified role Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cep192-plk4-6 29 36 unspecified role unspecified role unspecified role unspecified role Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 35 unspecified role unspecified role unspecified role unspecified role Region 201-258 Region 201-258 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction plk4-cep192-4 15 35 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association cep192-plk4-7 17 36 unspecified role unspecified role prey prey Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 35 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 201-258 Region 201-258 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cep192-plk4-8 28 35 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 36 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association plk4-cep192-5 19 35 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Region 201-280 Region 201-280 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O00444:p.Lys625Ala K625A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K625A'. Wed May 02 15:49:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys625ala' Tue Jun 30 20:06:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys625Ala' O00444:p.Arg684Ala R684A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R684A'. Wed May 02 14:45:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg684ala' Tue Jun 30 16:35:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg684Ala' O00444:p.Lys685Ala K685A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K685A'. Wed May 02 20:11:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys685ala' Tue Jun 30 17:13:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys685Ala' human Homo sapiens Human physical association physical association plk4-cep152-8 26 32 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association cep152-plk4-10 14 32 unspecified role unspecified role unspecified role unspecified role Region 1-60 Region 1-60 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 36 unspecified role unspecified role unspecified role unspecified role Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cep192-plk4-9 6 36 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 35 unspecified role unspecified role bait bait Region 201-238 Region 201-238 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association plk4-cep152-9 25 32 unspecified role unspecified role prey prey Region 1-217 Region 1-217 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 36 unspecified role unspecified role bait bait Region 580-970 Region 580-970 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction plk4-cep152-10 8 32 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human 36 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association cep192-plk4-10 20 36 unspecified role unspecified role unspecified role unspecified role Region 580-808 Region 580-808 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 35 unspecified role unspecified role unspecified role unspecified role Region 201-258 Region 201-258 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction