DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266984X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266982X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266980X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266977X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266981X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266978X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266976X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266983X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266986X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions in vitro In vitro peptide array peptide array autoradiography autoradiography Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266985X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. Nature cell biology 2014 imex curation Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Romano D., Nguyen LK., Matallanas D., Halasz M., Doherty C., Kholodenko BN., Kolch W. DIP original experiment: DIP-266979X Nature cell biology 2014 IMEx Protein interaction switches coordinate Raf-1 and MST2/Hippo signalling. raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 stk3_mouse Serine/threonine-protein kinase 3 Mst2 Stk3 Mess1 Mammalian STE20-like protein kinase 2 STE20-like kinase MST2 protein protein mouse Mus musculus Mouse MEQPPASKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLTEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKSPEQRATATQLLQHPFIKNAKPVSILRDLIAEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSSESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEEEEEEEEEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCDQSMREPGPMSNSVFPDNWRVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELHQRYSAKRQPILDAMDAKKRRQQNF DIP protein Q9JI10 original sequence version: 120 9CDD365437581665 ksr1_human Kinase suppressor of Ras 1 KSR1 KSR protein protein human Homo sapiens Human MDRAALRAAAMGEKKEGGGGGDAAAAEGGAGAAASRALQQCGQLQKLIDISIGSLRGLRTKCAVSNDLTQQEIRTLEAKLVRYICKQRQCKLSVAPGERTPELNSYPRFSDWLYTFNVRPEVVQEIPRDLTLDALLEMNEAKVKETLRRCGASGDECGRLQYALTCLRKVTGLGGEHKEDSSWSSLDARRESGSGPSTDTLSAASLPWPPGSSQLGRAGNSAQGPRSISVSALPASDSPTPSFSEGLSDTCIPLHASGRLTPRALHSFITPPTTPQLRRHTKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVSSMRFDLSHGSPQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDSRFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAAPLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIVREMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIGSGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLNRRLSHPGHFWKSADINSSKVVPRFERFGLGVLESSNPKM DIP protein Q8IVT5 original sequence version: 115 61BF0F1598349540 rash_human GTPase HRas HRAS HRAS1 Transforming protein p21 p21ras H-Ras-1 c-H-ras Ha-Ras protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS DIP protein P01112 original sequence version: 91 DIP protein P01112 original sequence version: 106 DIP protein P01112 original sequence version: 144 DIP protein P01112 original sequence version: 118 DIP protein P01112 original sequence version: 143 DIP protein P01112 original sequence version: 152 DIP protein P01112-1 original sequence version: 159 DIP protein P01112 original sequence version: 170 DIP protein P01112-1 original sequence version: 185 DIP protein P01112 original sequence version: 192 DIP protein P01112-1 original sequence version: 196 DIP protein P01112-1 original sequence version: 202 EE6DC2D933E2856A raf1_mouse RAF proto-oncogene serine/threonine-protein kinase Raf1 Craf Proto-oncogene c-RAF Raf-1 protein protein mouse Mus musculus Mouse MEHIQGAWKTISNGFGLKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDSSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLQEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTVGDSGVPAPPSFPMRRMRESVSRMPASSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLHVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPGSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQLQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDDNPFSFQSDVYSYGIVLYELMAGELPYAHINNRDQIIFMVGRGYASPDLSRLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein Q99N57 original sequence version: 97 DIP protein Q99N57 original sequence version: 132 DIP protein Q99N57 original sequence version: 92 B70104AEF51C44A5 stk3_human Serine/threonine-protein kinase 3 STE20-like kinase MST2 Mammalian STE20-like protein kinase 2 Serine/threonine-protein kinase Krs-1 STK3 MST2 KRS1 protein protein human Homo sapiens Human MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF DIP protein Q13188 original sequence version: 150 DIP protein Q13188 original sequence version: 139 84F598ED3617292C raf1-stk3-1 7 17 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association raf1-stk3-2 5 17 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association raf1-stk3-3 9 13 unspecified role unspecified role prey prey mouse Mus musculus Mouse 12 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range mouse Mus musculus Mouse physical association physical association hras-raf1-8 11 15 unspecified role unspecified role bait bait Au5-tag Au5-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 12 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association raf1-ksr1-1 1 14 unspecified role unspecified role prey prey human Homo sapiens Human 12 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association stk3-raf1-1 4 17 unspecified role unspecified role bait bait human Homo sapiens Human 12 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association stk3-raf1-2 3 16 unspecified role unspecified role prey prey mouse Mus musculus Mouse 13 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association stk3-raf1-3 2 17 unspecified role unspecified role bait bait human Homo sapiens Human 12 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association raf1-hras-11 6 15 unspecified role unspecified role prey prey Au5-tag Au5-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 12 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association raf1-stk3-4 8 17 unspecified role unspecified role prey prey ecolx Escherichia coli 12 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction raf1-stk3-5 10 17 unspecified role unspecified role prey prey Region 140-170 Region 140-170 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 40-80 Region 40-80 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 86-116 Region 86-116 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 381-416 Region 381-416 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction