UniProt UniProt http://www.uniprot.org RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. Journal of virology 2014 imex curation Feng J., De Jesus PD., Su V., Han S., Gong D., Wu NC., Tian Y., Li X., Wu TT., Chanda SK., Sun R. Origin of RIOK3 according plasmid supplier (DNASU) rsun@mednet.ucla.edu Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Feng J., De Jesus PD., Su V., Han S., Gong D., Wu NC., Tian Y., Li X., Wu TT., Chanda SK., Sun R. Origin of RIOK3 according plasmid supplier (DNASU) rsun@mednet.ucla.edu Accepted 2017-FEB-06 AT 10:53 GMT AT 10:53 GMT by ORCHARD Journal of virology 2014 imex curation RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. Journal of virology 2014 imex curation Feng J., De Jesus PD., Su V., Han S., Gong D., Wu NC., Tian Y., Li X., Wu TT., Chanda SK., Sun R. Origin of RIOK3 according plasmid supplier (DNASU) rsun@mednet.ucla.edu Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Feng J., De Jesus PD., Su V., Han S., Gong D., Wu NC., Tian Y., Li X., Wu TT., Chanda SK., Sun R. Origin of RIOK3 according plasmid supplier (DNASU) rsun@mednet.ucla.edu Accepted 2017-FEB-06 AT 10:53 GMT AT 10:53 GMT by ORCHARD Journal of virology 2014 imex curation RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. Journal of virology 2014 imex curation Feng J., De Jesus PD., Su V., Han S., Gong D., Wu NC., Tian Y., Li X., Wu TT., Chanda SK., Sun R. Origin of RIOK3 according plasmid supplier (DNASU) rsun@mednet.ucla.edu Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Feng J., De Jesus PD., Su V., Han S., Gong D., Wu NC., Tian Y., Li X., Wu TT., Chanda SK., Sun R. Origin of RIOK3 according plasmid supplier (DNASU) rsun@mednet.ucla.edu Accepted 2017-FEB-06 AT 10:53 GMT AT 10:53 GMT by ORCHARD Journal of virology 2014 imex curation RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. RIOK3 is an adaptor protein required for IRF3-mediated antiviral type I interferon production. Journal of virology 2014 imex curation Feng J., De Jesus PD., Su V., Han S., Gong D., Wu NC., Tian Y., Li X., Wu TT., Chanda SK., Sun R. Origin of RIOK3 according plasmid supplier (DNASU) rsun@mednet.ucla.edu Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Feng J., De Jesus PD., Su V., Han S., Gong D., Wu NC., Tian Y., Li X., Wu TT., Chanda SK., Sun R. Origin of RIOK3 according plasmid supplier (DNASU) rsun@mednet.ucla.edu Accepted 2017-FEB-06 AT 10:53 GMT AT 10:53 GMT by ORCHARD Journal of virology 2014 imex curation tbk1_human Serine/threonine-protein kinase TBK1 TBK1 NAK TANK-binding kinase 1 T2K NF-kappa-B-activating kinase protein protein human Homo sapiens Human MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMDGGLRNVDCL DIP protein Q9UHD2 original sequence version: 116 DIP protein Q9UHD2 original sequence version: 120 DIP protein Q9UHD2 original sequence version: 79 DIP protein Q9UHD2 original sequence version: null B58E4FE1B502276D irf3_human Interferon regulatory factor 3 IRF3 protein protein human Homo sapiens Human MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVFDLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHPLSLTSDQYKAYLQDLVEGMDFQGPGES DIP protein Q14653 original sequence version: 96 DIP protein Q14653 original sequence version: 75 DIP protein Q14653 original sequence version: 95 DIP protein Q14653-1 original sequence version: 128 DIP protein Q14653 original sequence version: 119 F536676FA78B0110 riok3_human Serine/threonine-protein kinase RIO3 sudD homolog RIOK3 SUDD RIO kinase 3 protein protein human Homo sapiens Human MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE 3C3392BAC4ECB912 irf3-riok3-1 3 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4A tbk1-riok3-1 4 5 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4B riok3-irf3-2 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4C riok3-irf3-1 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4C, 5D siTBK1 cotransfected irf3-tbk1-4 1 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role ancillary ancillary association association 4A Requires RIOK3; not observed upon cotransfection with siRIOK3. riok3-irf3-3 4 6 unspecified role unspecified role unspecified role unspecified role nucl transfection nucleic acid transfection nucl transfection region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range range range 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5A riok3-irf3-4 2 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection O14730:p.Lys290Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:01:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys290ala' Tue Jun 30 19:29:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys290Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined A FLAG-RIOK3 lys290ala mutant also precipitated endogenous IRF3 and cotransfected TBK1 association association 6D