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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="14681454" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579232" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Distinct docking and stabilization steps of the Pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibers.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="24685147" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="doi" dbAc="MI:0574" id="10.1016/j.str.2014.03.001" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16098834" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-22671" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Distinct docking and stabilization steps of the Pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibers.</attribute>
            <attribute name="journal" nameAc="MI:0885">Structure (London, England : 1993)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2014</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Nivaskumar M.,  Bouvier G.,  Campos M.,  Nadeau N.,  Yu X.,  Egelman EH.,  Nilges M.,  Francetic O.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-22671" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-260636X" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="24685147" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="doi" dbAc="MI:0574" id="10.1016/j.str.2014.03.001" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="562">
            <names>
              <shortLabel>ecolx</shortLabel>
              <fullName>Escherichia coli</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>adenylate cyclase</shortLabel>
            <fullName>adenylate cyclase complementation</fullName>
            <alias type="go synonym" typeAc="MI:0303">bacterial two-hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">bacterial two-hybrid</alias>
            <alias type="synonym" typeAc="MI:1041">adenylate cyclase</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0014" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1004" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="9576956" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Nivaskumar M., Bouvier G., Campos M., Nadeau N., Yu X., Egelman EH., Nilges M., Francetic O.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-260636X</attribute>
          <attribute name="journal" nameAc="MI:0885">Structure (London, England : 1993)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2014</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Distinct docking and stabilization steps of the Pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibers.</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>q7bsv8_eco57</shortLabel>
          <fullName>Type II secretion system core protein G</fullName>
          <alias type="gene name" typeAc="MI:0301">etpG</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q7BSV8" version="3" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="BAA31762" version="TrEMBL_139" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="A0A4Q2TTT7" version="TrEMBL_135" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="A0A0T7C0U6" version="TrEMBL_104" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="A0A2P0HM01" version="TrEMBL_117" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15864371" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="BAA31762" version="TrEMBL_139" refType="gene"/>
          <secondaryRef db="ensemblbacteria" id="BAA31762" version="TrEMBL_139" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" version="TrEMBL_139"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0015627" version="TrEMBL_139"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0015628" version="TrEMBL_139"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" version="TrEMBL_139"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="TrEMBL_139"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0046872" version="TrEMBL_139"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000983"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR010054"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR012902"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR013545"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3G20"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_052612.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_001231211.1"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="83334">
          <names>
            <shortLabel>eco57</shortLabel>
            <fullName>Escherichia coli O157:H7</fullName>
          </names>
        </organism>
        <sequence>MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKVMSDLVALESTLDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPWGGDYQLLNPGQYSDIDIFSPGPDGVPNTEDDIGNWTLGNAQP</sequence>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-22671-1">
        <names>
          <shortLabel>pulg-pulg-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-188607E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16098853" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-22671-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="5">
                <names>
                  <shortLabel>Q7BSV8:p.[Glu52Ala;Asp56Ala]</shortLabel>
                  <fullName>E50A/D54A</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-16098859" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting strength</shortLabel>
                    <fullName>mutation disrupting interaction strength</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting strength</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1128" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528001" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>alanine scanning</shortLabel>
                    <fullName>alanine scanning</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456759" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="56"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="56"/>
                    <resultingSequence>
                      <originalSequence>D</originalSequence>
                      <newSequence>A</newSequence>
                    </resultingSequence>
                  </featureRange>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="52"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="52"/>
                    <resultingSequence>
                      <originalSequence>E</originalSequence>
                      <newSequence>A</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">Wed May 02 19:22:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu52ala,asp56ala'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 19:28:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu52Ala;Asp56Ala]'</attribute>
                </attributeList>
              </feature>
              <feature id="6">
                <names>
                  <shortLabel>Q7BSV8:p.Arg95_Arg96delinsAlaAla</shortLabel>
                  <fullName>R93A/R94A</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-16098862" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting strength</shortLabel>
                    <fullName>mutation disrupting interaction strength</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting strength</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1128" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528001" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>alanine scanning</shortLabel>
                    <fullName>alanine scanning</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456759" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="95"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="96"/>
                    <resultingSequence>
                      <originalSequence>RR</originalSequence>
                      <newSequence>AA</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">Wed May 02 18:18:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg95-96ala_ala'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 18:05:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg95_Arg96delinsAlaAla'</attribute>
                </attributeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
          <participant id="7">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>physical association</shortLabel>
            <fullName>physical association</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0915" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813147" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>