DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259813X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259808X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259809X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions in vitro In vitro hydroxylase assay hydroxylase assay predetermined predetermined participant predetermined Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259810X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259814X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259807X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions in vitro In vitro hydroxylase assay hydroxylase assay predetermined predetermined participant predetermined Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259811X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259806X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. PLoS biology 2014 imex curation Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions in vitro In vitro hydroxylase assay hydroxylase assay predetermined predetermined participant predetermined Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259812X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 roa2_human Heterogeneous nuclear ribonucleoproteins A2/B1 HNRNPA2B1 HNRPA2B1 protein protein human Homo sapiens Human MEKTLETVPLERKKREKEQFRKLFIGGLSFETTEESLRNYYEQWGKLTDCVVMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREESGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGKKRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKALSRQEMQEVQSSRSGRGGNFGFGDSRGGGGNFGPGPGSNFRGGSDGYGSGRGFGDGYNGYGGGPGGGNFGGSPGYGGGRGGYGGGGPGYGNQGGGYGGGYDNYGGGNYGSGNYNDFGNYNQQPSNYGPMKSGNFGGSRNMGGPYGGGNYGPGGSGGSGGYGGRSRY DIP protein P22626 original sequence version: 152 DIP protein P22626 original sequence version: 159 4C2560A3D8E99D62 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG q16630-1 Cleavage and polyadenylation specificity factor subunit 6 CPSF6 CFIM68 Cleavage and polyadenylation specificity factor 68 kDa subunit Pre-mRNA cleavage factor Im 68 kDa subunit Protein HPBRII-4/7 Cleavage factor Im complex 68 kDa subunit protein protein human Homo sapiens Human MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKLMDLLPKRELHGQNPVVTPCNKQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGSSRAAFPQGGRGRGRFPGAVPGGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGAPPPAPHVNPAFFPPPTNSGMPTSDSRGPPPTDPYGRPPPYDRGDYGPPGREMDTARTPLSEAEFEEIMNRNRAISSSAISRAVSDASAGDYGSAIETLVTAISLIKQSKVSADDRCKVLISSLQDCLHGIESKSYGSGSRRERSRERDHSRSREKSRRHKSRSRDRHDDYYRERSRERERHRDRDRDRDRERDREREYRHR DIP protein Q16630-1 original sequence version: 94 DIP protein Q16630 original sequence version: 123 721A5DA1B456AA79 q9y383-1 Putative RNA-binding protein Luc7-like 2 LUC7L2 CGI-59 CGI-74 protein protein human Homo sapiens Human MSAQAQMRAMLDQLMGTSRDGDTTRQRIKFSDDRVCKSHLLNCCPHDVLSGTRMDLGECLKVHDLALRADYEIASKEQDFFFELDAMDHLQSFIADCDRRTEVAKKRLAETQEEISAEVAAKAERVHELNEEIGKLLAKVEQLGAEGNVEESQKVMDEVEKARAKKREAEEVYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIEIREKLEELKRVVAEKQEKRNQERLKRREEREREEREKLRRSRSHSKNPKRSRSREHRRHRSRSMSRERKRRTRSKSREKRHRHRSRSSSRSRSRSHQRSRHSSRDRSRERSKRRSSKERFRDQDLASCDRDRSSRDRSPRDRDRKDKKRSYESANGRSEDRRSSEEREAGEI DIP protein Q9Y383-1 original sequence version: 85 DIP protein Q9Y383 original sequence version: 113 1C559CCE0F23F693 mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP DIP protein Q00987 original sequence version: 93 DIP protein Q00987-1 original sequence version: 192 DIP protein Q00987 original sequence version: 88 DIP protein Q00987 original sequence version: 180 DIP protein Q00987 original sequence version: 188 DIP protein Q00987 original sequence version: 189 DIP protein Q00987 original sequence version: 115 DIP protein Q00987-1 original sequence version: null DIP protein Q00987 original sequence version: 149 DIP protein Q00987 original sequence version: 146 DIP protein Q00987 original sequence version: 140 DIP protein Q00987 original sequence version: 183 DIP protein Q00987 original sequence version: 145 DIP protein Q00987 original sequence version: 182 DIP protein Q00987 original sequence version: 151 DIP protein Q00987 original sequence version: 156 DIP protein Q00987-1 original sequence version: 160 DIP protein Q00987 original sequence version: 160 DIP protein Q00987 original sequence version: 168 DIP protein Q00987 original sequence version: 186 DIP protein Q00987 original sequence version: 206 F37CE163876BC983 jmjd6_human Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 PTDSR KIAA0585 JMJD6 Histone arginine demethylase JMJD6 JmjC domain-containing protein 6 Jumonji domain-containing protein 6 Lysyl-hydroxylase JMJD6 Peptide-lysine 5-dioxygenase JMJD6 Phosphatidylserine receptor PSR protein protein human Homo sapiens Human MNHKSKKRIREAKRSARPELKDSLDWTRHNYYESFSLSPAAVADNVERADALQLSVEEFVERYERPYKPVVLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEHPKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPPRSGTGIHIDPLGTSAWNALVQGHKRWCLFPTSTPRELIKVTRDEGGNQQDEAITWFNVIYPRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDTTIAITQNFASSTNFPVVWHKTVRGRPKLSRKWYRILKQEHPELAVLADSVDLQESTGIASDSSSDSSSSSSSSSSDSDSECESGSEGDGTVHRRKKRRTCSMVGNGDTTSQDDCVSKERSSSR DIP protein Q6NYC1 original sequence version: 99 mint homomint Arginine demethylase which demethylates histone H3 at 'Arg-3' and histone H4 at 'Arg-4'. Required for differentiation of multiple organs during embryogenesis. Probably acts as a key regulator of hematopoietic differentiation. Seems to be necessary for the regulation of macrophage cytokine responses 9C9AADA98B24B035 mdm4_human Protein Mdm4 MDM4 MDMX p53-binding protein Mdm4 Mdm2-like p53-binding protein Protein Mdmx Double minute 4 protein protein protein human Homo sapiens Human MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA DIP protein O15151 original sequence version: 141 DIP protein O15151 original sequence version: 109 DIP protein O15151 original sequence version: 129 DIP protein O15151 original sequence version: 147 415E6FA5A0C69857 jmjd6-cpsf6-1 8 14 unspecified role unspecified role prey prey human Homo sapiens Human 16 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 11 unspecified role unspecified role prey prey human Homo sapiens Human 13 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role prey prey human Homo sapiens Human association association jmjd6-tp53-1 4 16 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 10 enzyme target enzyme target substrate substrate unspecified role unspecified role K382OH K382OH hy1lys monohydroxylated lysine Hy1Lys Hydroxy Lysyl (-Hyl-) Oxidation hydroxylationk modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm ecolx Escherichia coli hydroxylation hydroxylation reaction hydroxylation tp53-mdm2-33 5 17 unspecified role unspecified role prey prey human Homo sapiens Human 15 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait human Homo sapiens Human association association tp53-jmjd6-1 2 16 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association jmjd6-tp53-2 9 16 enzyme enzyme unspecified role unspecified role Q6NYC1:p.[His187Ala;Asp189Ala] H187A/D189A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H187A/D189A'. Wed May 02 16:15:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his187ala,asp189ala' Tue Jun 30 15:51:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His187Ala;Asp189Ala]' ecolx Escherichia coli 10 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 381-393 Region 381-393 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain K382OH K382OH hy1lys monohydroxylated lysine Hy1Lys Hydroxy Lysyl (-Hyl-) Oxidation hydroxylationk modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro hydroxylation hydroxylation reaction hydroxylation jmjd6-tp53-3 7 16 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 10 enzyme target enzyme target substrate substrate unspecified role unspecified role K382OH K382OH hy1lys monohydroxylated lysine Hy1Lys Hydroxy Lysyl (-Hyl-) Oxidation hydroxylationk modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm Region 381-393 Region 381-393 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P04637:p.Lys382Ala K382A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K382A'. Wed May 02 18:15:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys382ala' Tue Jun 30 16:00:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys382Ala' P04637:p.Lys382Arg K382R mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K R This feature has been corrected as a result of our quality control procedures. The original label was 'K382R'. Wed May 02 18:24:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys382arg' Tue Jun 30 18:38:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys382Arg' K382ac K382ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm K382me1 K382me1 me1lys monomethylated L-lysine Me1Lys Methyl Methylation methylk certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm in vitro In vitro hydroxylation hydroxylation reaction hydroxylation jmjd6-tp53-4 3 16 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role prey prey Region 290-393 Region 290-393 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction tp53-ub-15 1 12 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal human Homo sapiens Human 10 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association jmjd6-tp53-5 6 16 unspecified role unspecified role bait bait human Homo sapiens Human 10 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association