DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science (New York, N.Y.) 2014 imex curation Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266320X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science (New York, N.Y.) 2014 imex curation Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266322X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science (New York, N.Y.) 2014 imex curation Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266321X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science (New York, N.Y.) 2014 imex curation Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266319X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science (New York, N.Y.) 2014 imex curation Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266318X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science (New York, N.Y.) 2014 imex curation Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266323X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. bak1_arath BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 BAK1 SERK3 At4g33430 F17M5.190 Somatic embryogenesis receptor-like kinase 3 ELG Somatic embryogenesis receptor kinase 3 Protein ELONGATED protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERRLMIPCFFWLILVLDLVLRVSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCTWFHVTCNSDNSVTRVDLGNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNNSLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSLFTPISFANTKLTPLPASPPPPISPTPPSPAGSNRITGAIAGGVAAGAALLFAVPAIALAWWRRKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIGDSTSQIENEYPSGPR DIP protein Q94F62 original sequence version: 119 DIP protein Q94F62 original sequence version: 129 DIP protein Q94F62 original sequence version: 94 DIP protein Q94F62 original sequence version: 100 DIP protein Q94F62 original sequence version: 139 DIP protein Q94F62 original sequence version: 121 DIP protein Q94F62 original sequence version: 141 DIP protein Q94F62 original sequence version: 113 547B3F38FB46E1DD efr_arath LRR receptor-like serine/threonine-protein kinase EFR EFR Elongation factor Tu receptor F7C8.70 At5g20480 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLSFSLVFNALTLLLQVCIFAQARFSNETDMQALLEFKSQVSENNKREVLASWNHSSPFCNWIGVTCGRRRERVISLNLGGFKLTGVISPSIGNLSFLRLLNLADNSFGSTIPQKVGRLFRLQYLNMSYNLLEGRIPSSLSNCSRLSTVDLSSNHLGHGVPSELGSLSKLAILDLSKNNLTGNFPASLGNLTSLQKLDFAYNQMRGEIPDEVARLTQMVFFQIALNSFSGGFPPALYNISSLESLSLADNSFSGNLRADFGYLLPNLRRLLLGTNQFTGAIPKTLANISSLERFDISSNYLSGSIPLSFGKLRNLWWLGIRNNSLGNNSSSGLEFIGAVANCTQLEYLDVGYNRLGGELPASIANLSTTLTSLFLGQNLISGTIPHDIGNLVSLQELSLETNMLSGELPVSFGKLLNLQVVDLYSNAISGEIPSYFGNMTRLQKLHLNSNSFHGRIPQSLGRCRYLLDLWMDTNRLNGTIPQEILQIPSLAYIDLSNNFLTGHFPEEVGKLELLVGLGASYNKLSGKMPQAIGGCLSMEFLFMQGNSFDGAIPDISRLVSLKNVDFSNNNLSGRIPRYLASLPSLRNLNLSMNKFEGRVPTTGVFRNATAVSVFGNTNICGGVREMQLKPCIVQASPRKRKPLSVRKKVVSGICIGIASLLLIIIVASLCWFMKRKKKNNASDGNPSDSTTLGMFHEKVSYEELHSATSRFSSTNLIGSGNFGNVFKGLLGPENKLVAVKVLNLLKHGATKSFMAECETFKGIRHRNLVKLITVCSSLDSEGNDFRALVYEFMPKGSLDMWLQLEDLERVNDHSRSLTPAEKLNIAIDVASALEYLHVHCHDPVAHCDIKPSNILLDDDLTAHVSDFGLAQLLYKYDRESFLNQFSSAGVRGTIGYAAPEYGMGGQPSIQGDVYSFGILLLEMFSGKKPTDESFAGDYNLHSYTKSILSGCTSSGGSNAIDEGLRLVLQVGIKCSEEYPRDRMRTDEAVRELISIRSKFFSSKTTITESPRDAPQSSPQEWMLNTDMHTM DIP protein C0LGT6 original sequence version: 51 E0E0AE671DA9124D fls2_arath LRR receptor-like serine/threonine-protein kinase FLS2 FLS2 Protein FLAGELLIN-SENSING 2 Protein FLAGELLIN-SENSITIVE 2 MPL12.13 MPL12.8 At5g46330 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLLSKTFLILTLTFFFFGIALAKQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKRTRVILIILGSAAALLLVLLLVLILTCCKKKEKKIENSSESSLPDLDSALKLKRFEPKELEQATDSFNSANIIGSSSLSTVYKGQLEDGTVIAVKVLNLKEFSAESDKWFYTEAKTLSQLKHRNLVKILGFAWESGKTKALVLPFMENGNLEDTIHGSAAPIGSLLEKIDLCVHIASGIDYLHSGYGFPIVHCDLKPANILLDSDRVAHVSDFGTARILGFREDGSTTASTSAFEGTIGYLAPEFAYMRKVTTKADVFSFGIIMMELMTKQRPTSLNDEDSQDMTLRQLVEKSIGNGRKGMVRVLDMELGDSIVSLKQEEAIEDFLKLCLFCTSSRPEDRPDMNEILTHLMKLRGKANSFREDRNEDREV DIP protein Q9FL28 original sequence version: 98 DIP protein Q9FL28 original sequence version: 108 DIP protein Q9FL28 original sequence version: 74 DIP protein Q9FL28 original sequence version: 79 DIP protein Q9FL28 original sequence version: 116 DIP protein Q9FL28 original sequence version: 92 6AF93B467A339359 hopd2_psesm Effector protein hopD2 hopD2 hopA01 hopPtoD2 PSPTO_4722 Tyrosine-protein phosphatase hopPtoD2 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MNPLQPIQHSITNSQMSGGQQLEAEGSQAHNSYSHPDRISLSQLSQSAHLALDHLSTQPNTDHQRVASLVRNAVQDGKFQLQSSNDTQVTYKTSVCPPANADTMGAAHLINNELTVQARLNDQLEYDIVSAHLYGPSEAISIDASSPPSANDLASSGLSERTHLGMNRVLLRYAVPPRETEDQCVMVIDKMPPPKHGKMSFFRTTNDLSKLPLGMETGGLSDLKLAGCERISSVEQVKSIRAALGGGPLTVLDLREESHAIVNGLPITLRGPMDWANAGLSQVDGAARESAMITELKRTKSLTLVDANYVKGKKSNPQTTELKNLNVRSEREVVTEAGATYRRVAITDHNRPSPEATDELVDIMRHCLQANESLVVHCNGGRGRTTTAMIMVDMLKNARNHSAETLITRMAKLSYDYNMTDLGSISALKRPFLEDRLKFLQAFHDYARNNPSGLSLNWTQWRAKIALE DIP protein Q79LY0 original sequence version: 75 efr-1 5 8 putative self putative self unspecified role unspecified role Region 680-1031 Region 680-1031 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pTyr pTyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined resulting-ptm resulting-ptm resulting ptm resulting-ptm C0LGT6:p.Asp849Asn D849N mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D N This feature has been corrected as a result of our quality control procedures. The original label was 'D849N'. Wed May 02 16:12:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp849asn' Tue Jun 30 19:13:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp849Asn' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation true efr-bak1-1 1 7 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 8 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association fls2-hopd2-1 6 9 unspecified role unspecified role bait bait Region 870-1153 Region 870-1153 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 10 unspecified role unspecified role prey prey Q79LY0:p.Cys378Ser C378S mutation mutation certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C378S'. Wed May 02 16:37:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys378ser' Tue Jun 30 18:19:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys378Ser' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association efr-hopd2-1 3 10 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range nicbe Nicotiana benthamiana 8 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range nicbe Nicotiana benthamiana physical association physical association efr-hopd2-2 4 10 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 8 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 680-1031 Region 680-1031 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pTyr pTyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation efr-hopd2-3 2 10 unspecified role unspecified role prey prey Q79LY0:p.Cys378Ser C378S mutation mutation certain certain sequence position certain certain certain sequence position certain C S This feature has been corrected as a result of our quality control procedures. The original label was 'C378S'. Wed May 02 18:38:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys378ser' Tue Jun 30 18:59:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys378Ser' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role bait bait Region 712-1001 Region 712-1001 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association