DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Nature 2014 imex curation Peisley A., Wu B., Xu H., Chen ZJ., Hur S. Only protein-protein interactions ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel tag fluorescence tag visualisation by fluorescence tag fluorescence Peisley A., Wu B., Xu H., Chen ZJ., Hur S. DIP original experiment: DIP-260832X Nature 2014 IMEx Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. Nature 2014 imex curation Peisley A., Wu B., Xu H., Chen ZJ., Hur S. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Peisley A., Wu B., Xu H., Chen ZJ., Hur S. DIP original experiment: DIP-260833X Nature 2014 IMEx Structural basis for ubiquitin-mediated antiviral signal activation by RIG-I. ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG o95786-1 Antiviral innate immune response receptor RIG-I Probable ATP-dependent RNA helicase DDX58 DDX58 DEAD box protein 58 RIG-I-like receptor 1 Retinoic acid-inducible gene 1 protein Retinoic acid-inducible gene I protein protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK DIP protein O95786-1 original sequence version: 126 DIP protein O95786-1 original sequence version: 118 DIP protein O95786 original sequence version: 63 DIP protein O95786 original sequence version: 116 DIP protein O95786-1 original sequence version: 116 DIP protein O95786 original sequence version: 105 DIP protein O95786 original sequence version: 80 DIP protein O95786-1 original sequence version: 78 DIP protein O95786 original sequence version: 102 DIP protein O95786-1 original sequence version: 131 DIP protein O95786 original sequence version: 134 DIP protein O95786 original sequence version: 135 DIP protein O95786-1 original sequence version: 120 DIP protein O95786 original sequence version: 120 ddx58-7 1 4 unspecified role unspecified role unspecified role unspecified role Region 1-200 Region 1-200 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O95786-1:p.[Arg17Ala;Lys18Ala] R17A/K18A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R17A/K18A'. Wed May 02 14:46:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg17ala,lys18ala' Tue Jun 30 15:47:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg17Ala;Lys18Ala]' O95786-1:p.Asp21Ala D21A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D21A'. Wed May 02 18:36:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp21ala' Tue Jun 30 16:22:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp21Ala' O95786-1:p.[Glu35Ala;Glu36Ala] E35A/E36A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E35A/E36A'. Wed May 02 18:53:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu35ala,glu36ala' Tue Jun 30 16:30:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu35Ala;Glu36Ala]' O95786-1:p.[Lys108Ala;Arg109Ala] K108A/R109A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K108A/R109A'. Wed May 02 15:28:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys108ala,arg109ala' Tue Jun 30 19:35:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys108Ala;Arg109Ala]' O95786-1:p.[Glu137Ala;Glu138Ala] E137A/E138A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E137A/E138A'. Wed May 02 15:04:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu137ala,glu138ala' Tue Jun 30 15:50:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu137Ala;Glu138Ala]' O95786-1:p.Glu153Ala E153A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E153A'. Wed May 02 15:43:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu153ala' Tue Jun 30 18:29:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu153Ala' O95786-1:p.[Asp163Ala;Lys164Ala] D163A/K164A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D163A/K164A'. Wed May 02 14:29:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp163ala,lys164ala' Tue Jun 30 19:02:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp163Ala;Lys164Ala]' Alexa647-label Alexa647-label alexa label alexa label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction ddx58-ub-3 2 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role Region 1-200 Region 1-200 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction