DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Science (New York, N.Y.) 2014 imex curation Xu T., Dai N., Chen J., Nagawa S., Cao M., Li H., Zhou Z., Chen X., De Rycke R., Rakusová H., Wang W., Jones AM., Friml J., Patterson SE., Bleecker AB., Yang Z. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xu T., Dai N., Chen J., Nagawa S., Cao M., Li H., Zhou Z., Chen X., De Rycke R., Rakusová H., Wang W., Jones AM., Friml J., Patterson SE., Bleecker AB., Yang Z. DIP original experiment: DIP-264660X Science (New York, N.Y.) 2014 IMEx Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Science (New York, N.Y.) 2014 imex curation Xu T., Dai N., Chen J., Nagawa S., Cao M., Li H., Zhou Z., Chen X., De Rycke R., Rakusová H., Wang W., Jones AM., Friml J., Patterson SE., Bleecker AB., Yang Z. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Xu T., Dai N., Chen J., Nagawa S., Cao M., Li H., Zhou Z., Chen X., De Rycke R., Rakusová H., Wang W., Jones AM., Friml J., Patterson SE., Bleecker AB., Yang Z. DIP original experiment: DIP-264661X Science (New York, N.Y.) 2014 IMEx Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Science (New York, N.Y.) 2014 imex curation Xu T., Dai N., Chen J., Nagawa S., Cao M., Li H., Zhou Z., Chen X., De Rycke R., Rakusová H., Wang W., Jones AM., Friml J., Patterson SE., Bleecker AB., Yang Z. Only protein-protein interactions nicbe Nicotiana benthamiana pull down pull down western blot western blot Immuno blot Immuno blot Xu T., Dai N., Chen J., Nagawa S., Cao M., Li H., Zhou Z., Chen X., De Rycke R., Rakusová H., Wang W., Jones AM., Friml J., Patterson SE., Bleecker AB., Yang Z. DIP original experiment: DIP-264662X Science (New York, N.Y.) 2014 IMEx Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. tmk1_arath Receptor protein kinase TMK1 TMK1 BARK1-like kinase 1 BLK1 Transmembrane kinase 1 F15E12.4 At1g66150 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKKRRTFLLFSFTFLLLLSLSKADSDGDLSAMLSLKKSLNPPSSFGWSDPDPCKWTHIVCTGTKRVTRIQIGHSGLQGTLSPDLRNLSELERLELQWNNISGPVPSLSGLASLQVLMLSNNNFDSIPSDVFQGLTSLQSVEIDNNPFKSWEIPESLRNASALQNFSANSANVSGSLPGFLGPDEFPGLSILHLAFNNLEGELPMSLAGSQVQSLWLNGQKLTGDITVLQNMTGLKEVWLHSNKFSGPLPDFSGLKELESLSLRDNSFTGPVPASLLSLESLKVVNLTNNHLQGPVPVFKSSVSVDLDKDSNSFCLSSPGECDPRVKSLLLIASSFDYPPRLAESWKGNDPCTNWIGIACSNGNITVISLEKMELTGTISPEFGAIKSLQRIILGINNLTGMIPQELTTLPNLKTLDVSSNKLFGKVPGFRSNVVVNTNGNPDIGKDKSSLSSPGSSSPSGGSGSGINGDKDRRGMKSSTFIGIIVGSVLGGLLSIFLIGLLVFCWYKKRQKRFSGSESSNAVVVHPRHSGSDNESVKITVAGSSVSVGGISDTYTLPGTSEVGDNIQMVEAGNMLISIQVLRSVTNNFSSDNILGSGGFGVVYKGELHDGTKIAVKRMENGVIAGKGFAEFKSEIAVLTKVRHRHLVTLLGYCLDGNEKLLVYEYMPQGTLSRHLFEWSEEGLKPLLWKQRLTLALDVARGVEYLHGLAHQSFIHRDLKPSNILLGDDMRAKVADFGLVRLAPEGKGSIETRIAGTFGYLAPEYAVTGRVTTKVDVYSFGVILMELITGRKSLDESQPEESIHLVSWFKRMYINKEASFKKAIDTTIDLDEETLASVHTVAELAGHCCAREPYQRPDMGHAVNILSSLVELWKPSDQNPEDIYGIDLDMSLPQALKKWQAYEGRSDLESSTSSLLPSLDNTQMSIPTRPYGFAESFTSVDGR DIP protein P43298 original sequence version: 131 93E300B52FF549DE abp1_arath Auxin-binding protein 1 ERABP1 T4I9.14 At4g02980 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MIVLSVGSASSSPIVVVFSVALLLFYFSETSLGAPCPINGLPIVRNISDLPQDNYGRPGLSHMTVAGSVLHGMKEVEIWLQTFAPGSETPIHRHSCEEVFVVLKGSGTLYLAETHGNFPGKPIEFPIFANSTIHIPINDAHQVKNTGHEDLQVLVIISRPPIKIFIYEDWFMPHTAARLKFPYYWDEQCIQESQKDEL DIP protein P33487 original sequence version: 114 tmk1-erabp1-1 1 5 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 4 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association erabp1-tmk1-1 2 5 unspecified role unspecified role bait bait arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 4 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association tmk1-erabp1-2 3 5 unspecified role unspecified role prey prey nicbe Nicotiana benthamiana 4 unspecified role unspecified role bait bait Biotin-tag Biotin-tag biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 1-520 Region 1-520 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain nicbe Nicotiana benthamiana physical association physical association