DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. PLoS biology 2014 imex curation Mechaly AE., Sassoon N., Betton JM., Alzari PM. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mechaly AE., Sassoon N., Betton JM., Alzari PM. DIP original experiment: DIP-259773X PLoS biology 2014 IMEx Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. PLoS biology 2014 imex curation Mechaly AE., Sassoon N., Betton JM., Alzari PM. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Mechaly AE., Sassoon N., Betton JM., Alzari PM. DIP original experiment: DIP-259770X PLoS biology 2014 IMEx Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. PLoS biology 2014 imex curation Mechaly AE., Sassoon N., Betton JM., Alzari PM. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Mechaly AE., Sassoon N., Betton JM., Alzari PM. DIP original experiment: DIP-259771X PLoS biology 2014 IMEx Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. PLoS biology 2014 imex curation Mechaly AE., Sassoon N., Betton JM., Alzari PM. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mechaly AE., Sassoon N., Betton JM., Alzari PM. DIP original experiment: DIP-259772X PLoS biology 2014 IMEx Segmental helical motions and dynamical asymmetry modulate histidine kinase autophosphorylation. cpxa_ecoli Sensor histidine kinase CpxA ecfB eup ssd JW3882 b3911 cpxA protein protein ecoli Escherichia coli (strain K12) MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQRQGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEMQIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLLFDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEFLAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELERIETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTVNFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVSPEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIWLPLYKRS DIP protein P0AE82 original sequence version: 53 DIP protein P0AE82 original sequence version: 81 720EE4A62885BA33 cpxr_ecoli Transcriptional regulatory protein CpxR cpxR b3912 JW3883 yiiA protein protein ecoli Escherichia coli (strain K12) MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDSIDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPFNDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGTEFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWFKTLRGRGYLMVSAS DIP protein P0AE88 original sequence version: 47 DIP protein P0AE88 original sequence version: 42 DIP protein P0AE88 original sequence version: 78 667FC2B246082F46 cpxa-cpxr-3 1 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-115 Region 1-115 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role P0AE82:p.Met228Val M228V mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M V This feature has been corrected as a result of our quality control procedures. The original label was 'M228V'. Wed May 02 14:56:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met228val' Tue Jun 30 15:54:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met228Val' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation cpxa-1 2 5 unspecified role unspecified role unspecified role unspecified role Region 188-457 Region 188-457 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cpxa-2 3 5 unspecified role unspecified role unspecified role unspecified role Region 188-457 Region 188-457 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cpxa-3 4 5 putative self putative self unspecified role unspecified role Region 188-457 Region 188-457 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0AE82:p.Met228Val M228V mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M V This feature has been corrected as a result of our quality control procedures. The original label was 'M228V'. Wed May 02 18:26:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met228val' Tue Jun 30 18:30:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met228Val' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation true