DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Nature chemical biology 2014 imex curation Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. DIP original experiment: DIP-260175X Nature chemical biology 2014 IMEx E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Nature chemical biology 2014 imex curation Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. DIP original experiment: DIP-260174X Nature chemical biology 2014 IMEx E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Nature chemical biology 2014 imex curation Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. DIP original experiment: DIP-260173X Nature chemical biology 2014 IMEx E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. rbx1_human E3 ubiquitin-protein ligase RBX1 RBX1 RNF75 ROC1 Regulator of cullins 1 RING finger protein 75 Protein ZYP RING-box protein 1 E3 ubiquitin-protein transferase RBX1 protein protein human Homo sapiens Human MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH DIP protein P62877 original sequence version: 120 DIP protein P62877 original sequence version: 115 DIP protein P62877 original sequence version: 78 DIP protein P62877 original sequence version: 113 DIP protein P62877 original sequence version: 75 DIP protein P62877 original sequence version: 86 DIP protein P62877 original sequence version: 136 DIP protein P62877 original sequence version: 126 DIP protein P62877 original sequence version: 90 DIP protein P62877 original sequence version: 114 30FC5ADF66096C0E ub2r1_human Ubiquitin-conjugating enzyme E2 R1 Ubiquitin-protein ligase R1 Ubiquitin-conjugating enzyme E2-32 kDa complementing Ubiquitin-conjugating enzyme E2-CDC34 CDC34 UBE2R1 UBCH3 (E3-independent) E2 ubiquitin-conjugating enzyme R1 E2 ubiquitin-conjugating enzyme R1 protein protein human Homo sapiens Human MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES DIP protein P49427 original sequence version: 101 DIP protein P49427 original sequence version: 134 258960666B589DB3 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG cdc34-rbx1-1 1 4 unspecified role unspecified role unspecified role unspecified role Region 12-108 Region 12-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role P49427:p.Thr117Glu T117E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T E This feature has been corrected as a result of our quality control procedures. The original label was 'T117E'. Wed May 02 16:08:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr117glu' Tue Jun 30 20:17:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr117Glu' P49427:p.Tyr70Arg Y70R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y R This feature has been corrected as a result of our quality control procedures. The original label was 'Y70R'. Wed May 02 18:16:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr70arg' Tue Jun 30 17:46:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr70Arg' ecolx Escherichia coli direct interaction direct interaction cdc34-ub-3 3 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 7-184 Region 7-184 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cdc34-ub-4 2 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 7-184 Region 7-184 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P49427:p.Glu133Arg E133R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E R This feature has been corrected as a result of our quality control procedures. The original label was 'E133R'. Wed May 02 14:53:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu133arg' Tue Jun 30 20:13:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu133Arg' P49427:p.Ser129Arg S129R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S R This feature has been corrected as a result of our quality control procedures. The original label was 'S129R'. Wed May 02 19:16:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser129arg' Tue Jun 30 18:16:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser129Arg' ecolx Escherichia coli direct interaction direct interaction