DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Nature 2014 imex curation Murayama Y., Uhlmann F. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Murayama Y., Uhlmann F. DIP original experiment: DIP-259837X Nature 2014 IMEx Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Nature 2014 imex curation Murayama Y., Uhlmann F. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Murayama Y., Uhlmann F. DIP original experiment: DIP-259838X Nature 2014 IMEx Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Nature 2014 imex curation Murayama Y., Uhlmann F. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Murayama Y., Uhlmann F. DIP original experiment: DIP-259839X Nature 2014 IMEx Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Nature 2014 imex curation Murayama Y., Uhlmann F. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Murayama Y., Uhlmann F. DIP original experiment: DIP-259841X Nature 2014 IMEx Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Biochemical reconstitution of topological DNA binding by the cohesin ring. Nature 2014 imex curation Murayama Y., Uhlmann F. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Murayama Y., Uhlmann F. DIP original experiment: DIP-259840X Nature 2014 IMEx Biochemical reconstitution of topological DNA binding by the cohesin ring. smc3_schpo Structural maintenance of chromosomes protein 3 psm3 smc3 SPAC10F6.09c Cohesin complex Psm3 subunit protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MYITKIVIQGFKSYKDYTVIEPLSPHHNVIVGRNGSGKSNFFAAIRFVLSDAYTHLSREERQALLHEGPGATVMSAYVEVTFANADNRFPTGKSEVVLRRTIGLKKDEYSLDKKTVSKTEVINLLESAGFSRSNPYYIVPQGRVTSLTNAKDSERLELLKEVAGTQIYENRRAESNKIMDETIQKSEKIDELLQYIEERLRELEEEKNDLAVYHKKDNERRCLEYAIYSREHDEINSVLDALEQDRIAALERNDDDSGAFIQREERIERIKAEITELNHSLELLRVEKQQNDEDYTNIMKSKVALELQSSQLSRQIEFSKKDESSKLNILSELESKISEKENELSEILPKYNAIVSEADDLNKRIMLLKNQKQSLLDKQSRTSQFTTKKERDEWIRNQLLQINRNINSTKENSDYLKTEYDEMENELKAKLSRKKEIEISLESQGDRMSQLLANITSINERKENLTDKRKSLWREEAKLKSSIENVKDDLSRSEKALGTTMDRNTSNGIRAVKDIAERLKLEGYYGPLCELFKVDNRFKVAVEATAGNSLFHIVVDNDETATQILDVIYKENAGRVTFMPLNKLRPKAVTYPDASDALPLIQYLEFDPKFDAAIKQVFSKTIVCPSIETASQYARSHQLNGITLSGDRSDKKGALTAGYRDYRNSRLDAIKNVKTYQIKFSDLQESLEKCRSEIESFDQKITACLDDLQKAQLSLKQFERDHIPLKDELVTITGETTDLQESMHHKSRMLELVVLELHTLEQQANDLKSELSSEMDELDPKDVEALKSLSGQIENLSHEFDAIIKERAHIEARKTALEYELNTNLYLRRNPLKAEIGSDNRIDESELNSVKRSLLKYENKLQIIKSSSSGLEEQMQRINSEISDKRNELESLEELQHEVATRIEQDAKINERNAAKRSLLLARKKECNEKIKSLGVLPEEAFIKYVSTSSNAIVKKLHKINEALKDYGSVNKKAYEQFNNFTKQRDSLLARREELRRSQESISELTTVLDQRKDEAIERTFKQVAKSFSEIFVKLVPAGRGELVMNRRSELSQSIEQDISMDIDTPSQKSSIDNYTGISIRVSFNSKDDEQLNINQLSGGQKSLCALTLIFAIQRCDPAPFNILDECDANLDAQYRSAIAAMVKEMSKTSQFICTTFRPEMVKVADNFYGVMFNHKVSTVESISKEEAMAFVEG FD4D1E81A9E2E423 FD4D1E81A9E2E423 mis4_schpo Sister chromatid cohesion protein mis4 mis4 SCC2 homolog SPAC31A2.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLFEMTPETFKKVNSSNRIIKGLQYTPLASSIPLENGLQNVLYPSSKFQNEPLQLNSEESSIMQRYVDMLNPGATFVNDSETFNFYKNALSAMITEPAMPAMRVNASPVLDQKVCNSDSLSELNDFTKSLIQPSMLMCEPKEKPDASSINTNRSSSDNGFLTPSSSPRSPSCSRVFNAVQLCSPKKSKDDITTPKKRLMEDTYSPRESPSKIQRLQDVLLKQLQDTRLLISQVIEAENSEDFSSNSLFIKREDDDGKHISSHAIEKLYMALTKLSRLGACDKLLEEGSIILVKQILEKELKELPVACYSIINLHDSLTQFPNLDFILKTTALVLFIIFLVPSFKKLQNEESILHLLNILHSIFEYTVPEAIDNIVQSKTSDARTSEIQHLSVLLQKVANVLNILSKVAHEIPLSEAVVIRIVYLFPKVSTLDNSFKTKLPNCNSSSFDFLKAPLFQTLQYLFRLYPYQRDFIIEESLTNFSHLPTARSVSRTYRLSNGKSIQYYSTLFVRLIQSCSIQNLFDSEIVQSESKSTEALHSGNLTEHLKTVESILSKSRHEEYRIANHIVAYLLSRSLKQNKTESDNSFAILTKILLEDLLNMLSLPEWCGTETIIRQFAMNLVMTVTNDKQAVSSKNAALDLISLIVNKVLALFDLSLFEKHNIPAPTNFNDIISFIPSITRLNELSQVSFNHFYFLCKGDISLENILPYNYNKWFSFLLQLRKVCNDSEALKIIDNCIDKNMQKSQEGFQGPSPFKADENDEDIFIISLYHSSLFLNLKFFVSLIIGFLDSPQASLRTKCLRIINQMKTIPSILRTHPEVLAQIISKSNDQSAIVRDTVLDLLGTYIMAYRETIPQIYGCIISGISDPSTIVRKRAIKQLCEVYEATEDLNIRVDIASKLLTRSNDEEETISELSLEVLEKLWFSPASNELDCQKGYEQLTFLEKQKLRVQYFPILKLCAEPSTERHVLLVTSLKTMLTSKEEINLSTLHTQIRLLLSCLFNQLIEVVTEDQVDESTKGILYEIMSTLFVFSRAFPFLFDLSYLHLLKPYLRSASTIEEQRFLYYVVAIFRQVLPFQKEISESFLRSLESVLLQRLTKAGTATLMEIVPCLCSLFTRLNDYERLKKIVVSCLKSLEEARHSENNFQKMVRLIDLIGLFSRYGDLNRINDDWKHSLDFISPECDDAYVILLGYFQKLLKDAKGQLRIHIIDNMSRICLRETSLFISPLMLSTLDMIIAENNVNEVSVLFKSFLELLAADEDLIFEADQKLSLKGKQNVQSNKSVDRDMLKGTKDKQWIEGVSASLMQHFLPCILDSCFSKNLRYSMLGIEILKCIIHQGLVNPRMCFSTIIALESNAIKETREVAILLHTELHRRHESLIDGLYAQSADLIFSLQKTEEYQTFKLGEFSPFQSAYTIVSADKSSKSRKKLIMQILKPLKLDGIDLPSFTEEKVSFVSFCCVCLAGIPYVSIEEPLMIISTVDSVLATIGPTITGWMKKLDHERFKILAGINLCNLIYLKRYIKYAFSISDSSRPIREKKPLTLLNRGYVDLITSDAKPDIVSKLVIKLFEEENILSGEDQVEGEQLTVV 215B504316EB42D0 scc3_schpo Cohesin subunit psc3 SCC3 homolog psc3 SPAC17H9.20 SPAC607.01 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSESVTTGSDDDGGDRESSPVMLSQSFDPMSSSSNSSSEENSDDDYEKTISSKKRHPRPNSKGVNVKRSRRNAIVEEDPQEEIFNNLFAFLLDQKVDTMDIAVSWFADYAKDNQSALANLINFILKCCGCNRAINVFDVQDQDSASATLSQIQLSVERTSTRDYPLNSKNLKFRNFRKRLTGLLSNFVSQLSIRNYLYNSTVFEDIMSWVVAMSSSTMRPIRHTATVFCLNIMTFLCEKSKELLNEHAIATKQLEKEEKRSRVNRNRINELNNSLGEIVKQQDTLTTYLNDYFDSVFVHRYRDVEPKIRVDCLQELGVWINTVPSIFFSGSYLRYLGWMLSDINTTVRLTVVKVLRKFFETDSFIGGLRHFSSRFKERILEMSCVDADIGVRVASIRLCNAMRTCGFLENSEILKVLKLILDINPRVQREAVLFLCKVVDESVNEKIDLWGEEDYILKAFSQTSLTTFSVHWIKFSQMCKLLEEVRLSYQSSFDYDTLLRIFQKNGNFITPITQALLNACEIDSIYQSWEDISNFVLFDNYTSTLKDPIDSILSFCKLNDFQESILLQLLSASIQTVCNNNFITPKTVHNKQAAETTNDQNKDKDLLYLNLLPYINSITERNSASPTLLHDSLRLLFSMDLTEMTDPQLSRHFELLINNLKKFFLTNNDLQIIQGCTILFLRLDSIPALKEDLKLLVTDICDQTVTEFLKNFGSFNIQDAVITKDEFVIFEACLTRIEGCTSLKDFSDYPEFDIIYERLVSLLSRVPNSYEDTLKFSAINTLQSLLFWFFLRKDNPADEEKKKDDETKVFNCLINIMNNDSSKILQLQAARTFLETVIMKEGVKASHYNDDNRVSEEHNFLKPQFLDALLKILEGWLYTYAKVGQFPFKRLTQASSPHTQISLDKNPLNRRLLEHVCCDLTSKLLIVVSLSNTITPEFSQQFCELRGHYGPKLSAIVDEFLN F45C2C2A2E0443CC F45C2C2A2E0443CC psc3-mis4-1 3 8 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O13816:p.Arg300Gly R300G mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R G This feature has been corrected as a result of our quality control procedures. The original label was 'R300G'. Wed May 02 14:43:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg300gly' Tue Jun 30 18:01:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg300Gly' O13816:p.Pro306Leu P306L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain P L This feature has been corrected as a result of our quality control procedures. The original label was 'P306L'. Wed May 02 17:37:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro306leu' Tue Jun 30 19:49:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro306Leu' ecolx Escherichia coli 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast direct interaction direct interaction psm3-mis4-1 1 6 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast direct interaction direct interaction psc3-mis4-2 4 8 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O13816:p.Arg300Gly R300G mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R G This feature has been corrected as a result of our quality control procedures. The original label was 'R300G'. Wed May 02 18:46:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg300gly' Tue Jun 30 18:07:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg300Gly' O13816:p.Pro306Leu P306L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain P L This feature has been corrected as a result of our quality control procedures. The original label was 'P306L'. Wed May 02 19:51:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro306leu' Tue Jun 30 17:43:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro306Leu' ecolx Escherichia coli 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association psm3-mis4-2 2 6 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association psm3-mis4-3 5 6 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association