DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Mechanism of allosteric activation of SAMHD1 by dGTP. Mechanism of allosteric activation of SAMHD1 by dGTP. Nature structural & molecular biology 2013 imex curation Ji X., Wu Y., Yan J., Mehrens J., Yang H., DeLucia M., Hao C., Gronenborn AM., Skowronski J., Ahn J., Xiong Y. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ji X., Wu Y., Yan J., Mehrens J., Yang H., DeLucia M., Hao C., Gronenborn AM., Skowronski J., Ahn J., Xiong Y. DIP original experiment: DIP-255680X Nature structural & molecular biology 2013 IMEx Mechanism of allosteric activation of SAMHD1 by dGTP. Mechanism of allosteric activation of SAMHD1 by dGTP. Mechanism of allosteric activation of SAMHD1 by dGTP. Nature structural & molecular biology 2013 imex curation Ji X., Wu Y., Yan J., Mehrens J., Yang H., DeLucia M., Hao C., Gronenborn AM., Skowronski J., Ahn J., Xiong Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ji X., Wu Y., Yan J., Mehrens J., Yang H., DeLucia M., Hao C., Gronenborn AM., Skowronski J., Ahn J., Xiong Y. DIP original experiment: DIP-255681X Nature structural & molecular biology 2013 IMEx Mechanism of allosteric activation of SAMHD1 by dGTP. samh1_human Deoxynucleoside triphosphate triphosphohydrolase SAMHD1 Dendritic cell-derived IFNG-induced protein Monocyte protein 5 SAMHD1 MOP5 SAM domain and HD domain-containing protein 1 protein protein human Homo sapiens Human MQRADSEQPSKRPRCDDSPRTPSNTPSAEADWSPGLELHPDYKTWGPEQVCSFLRRGGFEEPVLLKNIRENEITGALLPCLDESRFENLGVSSLGERKKLLSYIQRLVQIHVDTMKVINDPIHGHIELHPLLVRIIDTPQFQRLRYIKQLGGGYYVFPGASHNRFEHSLGVGYLAGCLVHALGEKQPELQISERDVLCVQIAGLCHDLGHGPFSHMFDGRFIPLARPEVKWTHEQGSVMMFEHLINSNGIKPVMEQYGLIPEEDICFIKEQIVGPLESPVEDSLWPYKGRPENKSFLYEIVSNKRNGIDVDKWDYFARDCHHLGIQNNFDYKRFIKFARVCEVDNELRICARDKEVGNLYDMFHTRNSLHRRAYQHKVGNIIDTMITDAFLKADDYIEITGAGGKKYRISTAIDDMEAYTKLTDNIFLEILYSTDPKLKDAREILKQIEYRNLFKYVGETQPTGQIKIKREDYESLPKEVASAKPKVLLDVKLKAEDFIVDVINMDYGMQEKNPIDHVSFYCKTAPNRAIRITKNQVSQLLPEKFAEQLIRVYCKKVDRKSLYAARQYFVQWCADRNFTKPQDGDVIAPLITPQKKEWNDSTSVQNPTRLREASKSRVQLFKDDPM DIP protein Q9Y3Z3 original sequence version: 99 DIP protein Q9Y3Z3-1 original sequence version: 121 DIP protein Q9Y3Z3-1 original sequence version: 101 559CB6BB029E6558 samhd1-1 1 3 unspecified role unspecified role unspecified role unspecified role Region 113-626 Region 113-626 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction samhd1-2 2 3 unspecified role unspecified role unspecified role unspecified role Region 113-626 Region 113-626 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9Y3Z3:p.[Lys534Glu;Val537Asp;Leu540Asp] K534E/V537D/L540D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain V D certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K534E/V537D/L540D'. Wed May 02 20:07:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys534glu,val537asp,leu540asp' Tue Jun 30 17:31:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys534Glu;Val537Asp;Leu540Asp]' Q9Y3Z3:p.[Asp361Arg;His364Lys] D361R/H364K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain H K certain certain sequence position certain certain certain sequence position certain D R This feature has been corrected as a result of our quality control procedures. The original label was 'D361R/H364K'. Wed May 02 15:31:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp361arg,his364lys' Tue Jun 30 15:38:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp361Arg;His364Lys]' Q9Y3Z3:p.Arg333Ala R333A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R333A'. Wed May 02 19:22:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg333ala' Tue Jun 30 19:18:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg333Ala' Q9Y3Z3:p.[Arg352Ala;His376Ala;Lys377Ala] R352A/H376A/K377A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R352A/H376A/K377A'. Wed May 02 17:05:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg352ala,his376ala,lys377ala' Tue Jun 30 19:05:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg352Ala;His376Ala;Lys377Ala]' ecolx Escherichia coli direct interaction direct interaction