DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. Nature chemical biology 2013 imex curation Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. DIP original experiment: DIP-266861X Nature chemical biology 2013 IMEx The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. Nature chemical biology 2013 imex curation Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. DIP original experiment: DIP-266862X Nature chemical biology 2013 IMEx The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. Nature chemical biology 2013 imex curation Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. DIP original experiment: DIP-266860X Nature chemical biology 2013 IMEx The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. Nature chemical biology 2013 imex curation Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. DIP original experiment: DIP-266864X Nature chemical biology 2013 IMEx The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. Nature chemical biology 2013 imex curation Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Castro-Roa D., Garcia-Pino A., De Gieter S., van Nuland NA., Loris R., Zenkin N. DIP original experiment: DIP-266863X Nature chemical biology 2013 IMEx The Fic protein Doc uses an inverted substrate to phosphorylate and inactivate EF-Tu. eftu1_ecoli Elongation factor Tu 1 tufA b3339 P-43 JW3301 Bacteriophage Q beta RNA-directed RNA polymerase subunit III protein protein ecoli Escherichia coli (strain K12) MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG DIP protein P0CE47 original sequence version: 51 DIP protein P0CE47 original sequence version: null 731A60255F43358F doc_bpp1 Protein kinase doc doc Death on curing protein Toxin doc protein protein bpp1 Enterobacteria phage P1 Bacteriophage P1 MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE DIP protein Q06259 original sequence version: 71 E04A397538A595CD doc-tufa-1 3 6 enzyme target enzyme target substrate substrate unspecified role unspecified role P0CE47:p.Thr383Val T383V mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T V This feature has been corrected as a result of our quality control procedures. The original label was 'T383V'. Wed May 02 19:42:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr383val' Tue Jun 30 19:17:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr383Val' pT383 pT383 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation doc-tufa-2 2 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction doc-tufa-3 5 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction doc-tufa-4 1 6 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli 7 enzyme enzyme unspecified role unspecified role Q06259:p.His66Tyr H66Y mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain H Y This feature has been corrected as a result of our quality control procedures. The original label was 'H66Y'. Wed May 02 19:18:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his66tyr' Tue Jun 30 19:48:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His66Tyr' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation doc-tufa-5 4 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Q06259:p.Arg64Gly R64G mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R G This feature has been corrected as a result of our quality control procedures. The original label was 'R64G'. Wed May 02 16:34:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg64gly' Tue Jun 30 17:55:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg64Gly' ecolx Escherichia coli direct interaction direct interaction