DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266806X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266807X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266804X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266800X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266810X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266803X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266801X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266805X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266808X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266809X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266799X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Science (New York, N.Y.) 2013 imex curation Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sun Y., Li L., Macho AP., Han Z., Hu Z., Zipfel C., Zhou JM., Chai J. DIP original experiment: DIP-266802X Science (New York, N.Y.) 2013 IMEx Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. bak1_arath BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 BAK1 SERK3 At4g33430 F17M5.190 Somatic embryogenesis receptor-like kinase 3 ELG Somatic embryogenesis receptor kinase 3 Protein ELONGATED protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MERRLMIPCFFWLILVLDLVLRVSGNAEGDALSALKNSLADPNKVLQSWDATLVTPCTWFHVTCNSDNSVTRVDLGNANLSGQLVMQLGQLPNLQYLELYSNNITGTIPEQLGNLTELVSLDLYLNNLSGPIPSTLGRLKKLRFLRLNNNSLSGEIPRSLTAVLTLQVLDLSNNPLTGDIPVNGSFSLFTPISFANTKLTPLPASPPPPISPTPPSPAGSNRITGAIAGGVAAGAALLFAVPAIALAWWRRKKPQDHFFDVPAEEDPEVHLGQLKRFSLRELQVASDNFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTQGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPESQPPLDWPKRQRIALGSARGLAYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGVMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEALVDVDLQGNYKDEEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAERWEEWQKEEMFRQDFNYPTHHPAVSGWIIGDSTSQIENEYPSGPR DIP protein Q94F62 original sequence version: 119 DIP protein Q94F62 original sequence version: 129 DIP protein Q94F62 original sequence version: 94 DIP protein Q94F62 original sequence version: 100 DIP protein Q94F62 original sequence version: 139 DIP protein Q94F62 original sequence version: 121 DIP protein Q94F62 original sequence version: 141 DIP protein Q94F62 original sequence version: 113 547B3F38FB46E1DD flica_pseai A-type flagellin fliC flaA protein protein pseai Pseudomonas aeruginosa MALTVNTNIASLNTQRNLNNSSASLNTSLQRLSTGSRINSAKDDAAGLQIANRLTSQVNGLNVATKNANDGISLAQTAEGALQQSTNILQRMRDLSLQSANGSNSDSERTALNGEVKQLQKELDRISNTTTFGGRKLLDGSFGVASFQVGSAANEIISVGIDEMSAESLNGTYFKADGGGAVTAATASGTVDIAIGITGGSAVNVKVDMKGNETAEQAAAKIAAAVNDANVGIGAFSDGDTISYVSKAGKDGSGAITSAVSGVVIADTGSTGVGTAAGVTPSATAFAKTNDTVAKIDISTAKGAQSAVLVIDEAIKQIDAQRADLGAVQNRFDNTINNLKNIGENVSAARGRIEDTDFAAETANLTKNQVLQQAGTAILAQANQLPQSVLSLLR DIP protein P21184 original sequence version: 81 fls2_arath LRR receptor-like serine/threonine-protein kinase FLS2 FLS2 Protein FLAGELLIN-SENSING 2 Protein FLAGELLIN-SENSITIVE 2 MPL12.13 MPL12.8 At5g46330 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLLSKTFLILTLTFFFFGIALAKQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKRTRVILIILGSAAALLLVLLLVLILTCCKKKEKKIENSSESSLPDLDSALKLKRFEPKELEQATDSFNSANIIGSSSLSTVYKGQLEDGTVIAVKVLNLKEFSAESDKWFYTEAKTLSQLKHRNLVKILGFAWESGKTKALVLPFMENGNLEDTIHGSAAPIGSLLEKIDLCVHIASGIDYLHSGYGFPIVHCDLKPANILLDSDRVAHVSDFGTARILGFREDGSTTASTSAFEGTIGYLAPEFAYMRKVTTKADVFSFGIIMMELMTKQRPTSLNDEDSQDMTLRQLVEKSIGNGRKGMVRVLDMELGDSIVSLKQEEAIEDFLKLCLFCTSSRPEDRPDMNEILTHLMKLRGKANSFREDRNEDREV DIP protein Q9FL28 original sequence version: 98 DIP protein Q9FL28 original sequence version: 108 DIP protein Q9FL28 original sequence version: 74 DIP protein Q9FL28 original sequence version: 79 DIP protein Q9FL28 original sequence version: 116 DIP protein Q9FL28 original sequence version: 92 6AF93B467A339359 flic-bak1-1 9 13 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 14 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P21184:p.Gly47Tyr G47Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G Y This feature has been corrected as a result of our quality control procedures. The original label was 'G47Y'. Wed May 02 20:16:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly47tyr' Tue Jun 30 20:04:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly47Tyr' P21184:p.Gly47Ala G47A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain G A This feature has been corrected as a result of our quality control procedures. The original label was 'G47A'. Wed May 02 17:35:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly47ala' Tue Jun 30 17:01:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly47Ala' Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association bak1-flic-1 11 14 unspecified role unspecified role unspecified role unspecified role Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 13 unspecified role unspecified role unspecified role unspecified role Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 15 unspecified role unspecified role unspecified role unspecified role Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction flic-bak1-2 6 13 unspecified role unspecified role prey prey Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q94F62:p.Leu53Ala L53A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L53A'. Wed May 02 15:52:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu53ala' Tue Jun 30 17:49:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu53Ala' Q94F62:p.Val54Tyr V54Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V Y This feature has been corrected as a result of our quality control procedures. The original label was 'V54Y'. Wed May 02 18:14:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val54tyr' Tue Jun 30 19:18:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val54Tyr' Q94F62:p.Tyr96Ala Y96A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y96A'. Wed May 02 17:33:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr96ala' Tue Jun 30 19:07:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ala' Q94F62:p.[Phe60Ala;Phe144Ala] F60A/F144A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F60A/F144A'. Wed May 02 16:25:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe60ala,phe144ala' Tue Jun 30 20:22:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe60Ala;Phe144Ala]' trini Trichoplusia ni (Cabbage looper) Cabbage looper 14 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association bak1-fls2-4 1 13 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 1-249 Region 1-249 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 15 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association flic-fls2-1 12 14 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role prey prey Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction flic-fls2-2 10 14 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P21184:p.Asp43Ala D43A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D43A'. Wed May 02 15:57:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp43ala' Tue Jun 30 19:21:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp43Ala' P21184:p.Asp44Ala D44A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D44A'. Wed May 02 18:46:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp44ala' Tue Jun 30 16:50:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp44Ala' P21184:p.Ala46Tyr A46Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A Y This feature has been corrected as a result of our quality control procedures. The original label was 'A46Y'. Wed May 02 14:54:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala46tyr' Tue Jun 30 16:54:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala46Tyr' Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role prey prey Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction flic-bak1-3 7 13 unspecified role unspecified role prey prey Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 14 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role prey prey Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper association association fls2-bak1-3 5 13 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 15 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9FL28:p.[Arg294Ala;His316Ala] R294A/H316A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R294A/H316A'. Wed May 02 15:58:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg294ala,his316ala' Tue Jun 30 19:50:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg294Ala;His316Ala]' Q9FL28:p.Thr342Tyr T342Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T Y This feature has been corrected as a result of our quality control procedures. The original label was 'T342Y'. Wed May 02 18:51:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr342tyr' Tue Jun 30 17:51:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr342Tyr' Q9FL28:p.Ser437Tyr S437Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S Y This feature has been corrected as a result of our quality control procedures. The original label was 'S437Y'. Wed May 02 15:01:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser437tyr' Tue Jun 30 19:28:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser437Tyr' Q9FL28:p.Asp414Ala D414A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D414A'. Wed May 02 19:59:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp414ala' Tue Jun 30 19:49:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp414Ala' arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association fls2-bak1-4 2 13 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 15 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association flic-fls2-3 4 14 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role prey prey Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9FL28:p.[Arg294Ala;His316Ala] R294A/H316A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain H A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R294A/H316A'. Wed May 02 14:53:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg294ala,his316ala' Tue Jun 30 18:33:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg294Ala;His316Ala]' Q9FL28:p.Thr342Tyr T342Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T Y This feature has been corrected as a result of our quality control procedures. The original label was 'T342Y'. Wed May 02 16:23:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr342tyr' Tue Jun 30 18:44:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr342Tyr' Q9FL28:p.Ser437Tyr S437Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S Y This feature has been corrected as a result of our quality control procedures. The original label was 'S437Y'. Wed May 02 18:54:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser437tyr' Tue Jun 30 19:56:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser437Tyr' Q9FL28:p.Asp414Ala D414A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D414A'. Wed May 02 20:22:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp414ala' Tue Jun 30 16:26:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp414Ala' trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction fls2-bak1-5 3 13 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q94F62:p.Leu53Ala L53A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L53A'. Wed May 02 19:06:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu53ala' Tue Jun 30 18:40:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu53Ala' Q94F62:p.Val54Tyr V54Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V Y This feature has been corrected as a result of our quality control procedures. The original label was 'V54Y'. Wed May 02 16:02:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val54tyr' Tue Jun 30 17:58:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val54Tyr' Q94F62:p.[Phe60Ala;Phe144Ala] F60A/F144A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F60A/F144A'. Wed May 02 17:10:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe60ala,phe144ala' Tue Jun 30 19:18:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe60Ala;Phe144Ala]' Q94F62:p.Tyr96Ala Y96A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y96A'. Wed May 02 16:48:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr96ala' Tue Jun 30 17:34:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr96Ala' arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 15 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association bak1-flic-2 8 14 unspecified role unspecified role unspecified role unspecified role Region 30-51 Region 30-51 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 13 unspecified role unspecified role unspecified role unspecified role Region 1-220 Region 1-220 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 15 unspecified role unspecified role unspecified role unspecified role Region 25-800 Region 25-800 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association