IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch. Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch. Molecular and cellular biology 2013 imex curation Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE., Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE. Confocal microscopy expts (Fig 4) involve a fusion protein so not added hopper.65@osu.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE., Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE. Confocal microscopy expts (Fig 4) involve a fusion protein so not added Cross-linking agent - formaldehyde hopper.65@osu.edu Accepted 2017-MAR-16 AT 13:41 GMT AT 13:41 GMT by MGT Molecular and cellular biology 2013 imex curation E351K not L. Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch. Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch. Molecular and cellular biology 2013 imex curation Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE., Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE. Confocal microscopy expts (Fig 4) involve a fusion protein so not added hopper.65@osu.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE., Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE. Confocal microscopy expts (Fig 4) involve a fusion protein so not added hopper.65@osu.edu Accepted 2017-MAR-16 AT 13:41 GMT AT 13:41 GMT by MGT Molecular and cellular biology 2013 imex curation E351K not L. Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch. Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch. Molecular and cellular biology 2013 imex curation Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE., Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE. Confocal microscopy expts (Fig 4) involve a fusion protein so not added hopper.65@osu.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro bn-page blue native page bn-page weight by comassie molecular weight estimation by coomasie staining weight by comassie Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE., Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE. Confocal microscopy expts (Fig 4) involve a fusion protein so not added 4 to 16% gradient nondenaturing polyacrylamide gels containing ATP and galactose hopper.65@osu.edu Accepted 2017-MAR-16 AT 13:41 GMT AT 13:41 GMT by MGT Molecular and cellular biology 2013 imex curation E351K not L. Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch. Self-association of the Gal4 inhibitor protein Gal80 is impaired by Gal3: evidence for a new mechanism in the GAL gene switch. Molecular and cellular biology 2013 imex curation Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE., Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE. Confocal microscopy expts (Fig 4) involve a fusion protein so not added hopper.65@osu.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE., Egriboz O., Goswami S., Tao X., Dotts K., Schaeffer C., Pilauri V., Hopper JE. Confocal microscopy expts (Fig 4) involve a fusion protein so not added hopper.65@osu.edu Accepted 2017-MAR-16 AT 13:41 GMT AT 13:41 GMT by MGT Molecular and cellular biology 2013 imex curation E351K not L. gal80_yeast Galactose/lactose metabolism regulatory protein GAL80 GAL80 YM9958.12 YM9827.01 YML051W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSSQFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEVVMPLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKELISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMTSSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKGGKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLDPGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGFPTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL 1492473684F3894C 1492473684F3894C gal3_yeast Protein GAL3 YD8119.14 YDR009W GAL3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNTNVPIFSSPVRDLPRSFEQKHLAVVDAFFQTYHVKPDFIARSPGRVNLIGEHIDYCDFSVLPLAIDVDMLCAVKILDEKNPSITLTNADPKFAQRKFDLPLDGSYMAIDPSVSEWSNYFKCGLHVAHSYLKKIAPERFNNTPLVGAQIFCQSDIPTGGGLSSAFTCAAALATIRANMGKNFDISKKDLTRITAVAEHYVGVNNGGMDQATSVYGEEDHALYVEFRPKLKATPFKFPQLKNHEISFVIANTLVKSNKFETAPTNYNLRVIEVTVAANALATRYSVALPSHKDNSNSERGNLRDFMDAYYARYENQAQPWNGDIGTGIERLLKMLQLVEESFSRKKSGFTVHEASTALNCSREEFTRDYLTTFPVRFQVLKLYQRAKHVYSESLRVLKALKMMTSATFHTDEDFFTDFGRLMNESQASCDKLYECSCIETNQICSIALANGSFGSRLTGAGWGGCTIHLVPSGANGNVEQVRKALIEKFYNVRYPDLTDEELKDAIIVSKPALGTCLYEQ 5852028EBDDC0F3B 5852028EBDDC0F3B gal80-gal80-1 2 5 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A gal80-gal3-1 4 6 unspecified role unspecified role prey prey P13045:p.His199Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain H R Wed May 02 17:04:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his199arg' Tue Jun 30 17:43:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His199Arg' 5 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 1 Gal3 only bound to Gal80 in the presence of galactose and ATP. gal80-gal80-2 1 5 unspecified role unspecified role neutral component neutral component purified purified 5 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction Fig. 2 gal3-gal80-1 1 5 unspecified role unspecified role neutral component neutral component purified purified 6 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction Fig. 2 Interaction observed in the presence of 25 mM galactose gal3-gal80-2 3 5 unspecified role unspecified role neutral component neutral component purified purified P04387:p.Glu351Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E K Wed May 02 18:40:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu351lys' Tue Jun 30 15:45:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu351Lys' 6 unspecified role unspecified role neutral component neutral component purified purified direct interaction direct interaction Fig. 3 Interaction observed in the presence of 25 mM galactose gal80-gal80-3 3 5 unspecified role unspecified role neutral component neutral component purified purified P04387:p.Glu351Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:45:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu351lys' Tue Jun 30 17:27:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu351Lys' 5 unspecified role unspecified role neutral component neutral component purified purified P04387:p.Glu351Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:56:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu351lys' Tue Jun 30 19:44:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu351Lys' direct interaction direct interaction Fig. 3, supp fig 2 gal80-gal80-4 1 5 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Supp Fig. 1 Cross-linked 6×His-Gal80 oligomers were diminished in the presence of 6×His-Gal3 but not in the presence of 6×His-Gal3-E116G gal80-1 1 5 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Supp Fig. 1 Cross-linked 6×His-Gal80 oligomers were diminished in the presence of 6×His-Gal3 but not in the presence of 6×His-Gal3-E116G