DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. Nature 2013 imex curation Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. Only protein-protein interactions in vitro In vitro demyristoylation assay demyristoylase assay demyristoylation assay predetermined predetermined participant predetermined Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. DIP original experiment: DIP-252790X Nature 2013 IMEx SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. Nature 2013 imex curation Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. Only protein-protein interactions in vitro In vitro depalmitoylation assay depalmitoylase assay depalmitoylation assay predetermined predetermined participant predetermined Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. DIP original experiment: DIP-252791X Nature 2013 IMEx SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. Nature 2013 imex curation Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. Only protein-protein interactions in vitro In vitro demyristoylation assay demyristoylase assay demyristoylation assay predetermined predetermined participant predetermined Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. DIP original experiment: DIP-252793X Nature 2013 IMEx SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. Nature 2013 imex curation Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. Only protein-protein interactions in vitro In vitro demyristoylation assay demyristoylase assay demyristoylation assay predetermined predetermined participant predetermined Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. DIP original experiment: DIP-252792X Nature 2013 IMEx SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. Nature 2013 imex curation Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jiang H., Khan S., Wang Y., Charron G., He B., Sebastian C., Du J., Kim R., Ge E., Mostoslavsky R., Hang HC., Hao Q., Lin H. DIP original experiment: DIP-252789X Nature 2013 IMEx SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. sir6_human NAD-dependent protein deacetylase sirtuin-6 SIR2-like protein 6 SIRT6 SIR2L6 Regulatory protein SIR2 homolog 6 protein protein human Homo sapiens Human MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSSSVVFHTGAGISTASGIPDFRGPHGVWTMEERGLAPKFDTTFESARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRACRGELRDTILDWEDSLPDRDLALADEASRNADLSITLGTSLQIRPSGNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVPS DIP protein Q8N6T7 original sequence version: 100 DIP protein Q8N6T7-1 original sequence version: 105 DIP protein Q8N6T7 original sequence version: 137 0C86AAC497130BBF h3(413) Histone 3 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KQTARKSTGG h2bk12 Histone 2B peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) APAPKKGSKK tnfalpha TNF-alpha peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EALKKTGGPQ h3k9-sirt6-1 5 7 unspecified role unspecified role unspecified role unspecified role K6-myristoyl K6-myristoyl n6myrlys N6-myristoyl-L-lysine (2S)-2-amino-6-(tetradecanoylamino)hexanoic acid 2-amino-6-(tetradecanamido)hexanoic acid LIPID N6-myristoyl lysine N(zeta)-myristoyllysine N6-(1-oxotetradecyl)-L-lysine N6-(C14:0 aliphatic acyl)lysine N6-myristoyl-L-lysine N6-myristoylated L-lysine epsilon-myristoyllysine Myristoyl Myristoylation N6MyrLys myristoylationk certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role Region 1-294 Region 1-294 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3k9-sirt6-2 2 7 enzyme target enzyme target substrate substrate unspecified role unspecified role K6-palmitoyl K6-palmitoyl n6pamlys N6-palmitoyl-L-lysine (2S)-2-amino-6-(hexadecanoylamino)hexanoic acid 2-amino-6-(hexadecanamido)hexanoic acid LIPID N6-palmitoyl lysine N(zeta)-palmitoyllysine N6-(1-oxohexadecyl)-L-lysine N6-palmitoyl-L-lysine N6-palmitoylated L-lysine epsilon-palmitoyllysine N6-hexadecanoylated L-lysine N6PamLys Palmitoyl Palmitoylation palmitoylationk certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli depalmitoylation depalmitoylation reaction depalmitoylation h2bk12-sirt6-1 4 8 enzyme target enzyme target substrate substrate unspecified role unspecified role K6-myristoyl K6-myristoyl n6myrlys N6-myristoyl-L-lysine (2S)-2-amino-6-(tetradecanoylamino)hexanoic acid 2-amino-6-(tetradecanamido)hexanoic acid LIPID N6-myristoyl lysine N(zeta)-myristoyllysine N6-(1-oxotetradecyl)-L-lysine N6-(C14:0 aliphatic acyl)lysine N6-myristoyl-L-lysine N6-myristoylated L-lysine epsilon-myristoyllysine Myristoyl Myristoylation N6MyrLys myristoylationk certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli demyristoylation demyristoylation reaction demyristoylation h3k9-sirt6-3 1 7 enzyme target enzyme target substrate substrate unspecified role unspecified role K6-myristoyl K6-myristoyl n6myrlys N6-myristoyl-L-lysine (2S)-2-amino-6-(tetradecanoylamino)hexanoic acid 2-amino-6-(tetradecanamido)hexanoic acid LIPID N6-myristoyl lysine N(zeta)-myristoyllysine N6-(1-oxotetradecyl)-L-lysine N6-(C14:0 aliphatic acyl)lysine N6-myristoyl-L-lysine N6-myristoylated L-lysine epsilon-myristoyllysine Myristoyl Myristoylation N6MyrLys myristoylationk certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli demyristoylation demyristoylation reaction demyristoylation sirt6-tnfalpha-1 3 9 enzyme target enzyme target substrate substrate unspecified role unspecified role K5-myristoyl K5-myristoyl n6myrlys N6-myristoyl-L-lysine (2S)-2-amino-6-(tetradecanoylamino)hexanoic acid 2-amino-6-(tetradecanamido)hexanoic acid LIPID N6-myristoyl lysine N(zeta)-myristoyllysine N6-(1-oxotetradecyl)-L-lysine N6-(C14:0 aliphatic acyl)lysine N6-myristoyl-L-lysine N6-myristoylated L-lysine epsilon-myristoyllysine Myristoyl Myristoylation N6MyrLys myristoylationk certain certain sequence position certain certain certain sequence position certain K4-myristoyl K4-myristoyl n6myrlys N6-myristoyl-L-lysine (2S)-2-amino-6-(tetradecanoylamino)hexanoic acid 2-amino-6-(tetradecanamido)hexanoic acid LIPID N6-myristoyl lysine N(zeta)-myristoyllysine N6-(1-oxotetradecyl)-L-lysine N6-(C14:0 aliphatic acyl)lysine N6-myristoyl-L-lysine N6-myristoylated L-lysine epsilon-myristoyllysine Myristoyl Myristoylation N6MyrLys myristoylationk certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli demyristoylation demyristoylation reaction demyristoylation