DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structure (London, England : 1993) 2013 imex curation Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. DIP original experiment: DIP-252203X Structure (London, England : 1993) 2013 IMEx Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structure (London, England : 1993) 2013 imex curation Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. DIP original experiment: DIP-252206X Structure (London, England : 1993) 2013 IMEx Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structure (London, England : 1993) 2013 imex curation Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. DIP original experiment: DIP-252202X Structure (London, England : 1993) 2013 IMEx Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structure (London, England : 1993) 2013 imex curation Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. DIP original experiment: DIP-252204X Structure (London, England : 1993) 2013 IMEx Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. Structure (London, England : 1993) 2013 imex curation Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Virgili G., Frank F., Feoktistova K., Sawicki M., Sonenberg N., Fraser CS., Nagar B. DIP original experiment: DIP-252205X Structure (London, England : 1993) 2013 IMEx Structural analysis of the DAP5 MIF4G domain and its interaction with eIF4A. if4g1_human Eukaryotic translation initiation factor 4 gamma 1 p220 EIF4G1 EIF4G EIF4GI EIF4F protein protein human Homo sapiens Human MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN DIP protein Q04637 original sequence version: 68 DIP protein Q04637 original sequence version: 166 DIP protein Q04637 original sequence version: 160 DIP protein Q04637-1 original sequence version: 94 DIP protein Q04637 original sequence version: 115 DIP protein Q04637 original sequence version: 119 DIP protein Q04637 original sequence version: 146 DIP protein Q04637-1 original sequence version: 148 DIP protein Q04637 original sequence version: 150 324088B60863DA34 if4a1_human Eukaryotic initiation factor 4A-I EIF4A1 EIF4A DDX2A ATP-dependent RNA helicase eIF4A-1 protein protein human Homo sapiens Human MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI DIP protein P60842 original sequence version: 125 DIP protein P60842 original sequence version: 59 DIP protein P60842 original sequence version: 63 DIP protein P60842 original sequence version: 85 DIP protein P60842 original sequence version: 86 DIP protein P60842 original sequence version: 92 DIP protein P60842-1 original sequence version: 114 DIP protein P60842 original sequence version: 106 6EF89939F3045420 p78344-1 Eukaryotic translation initiation factor 4 gamma 2 EIF4G2 DAP5 Death-associated protein 5 p97 OK/SW-cl.75 protein protein human Homo sapiens Human MESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPGQNAQKWIPARSTRRDDNSAANNSANEKERHDAIFRKVRGILNKLTPEKFDKLCLELLNVGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLAEDAPNFDGPAAEGQPGQKQSTTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGNIKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQYFARMCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGMRSDFFLEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQSQFGEMGGKFMKSQGLSQLYHNQSQGLLSQLQGQSKDMPPRFSKKGQLNADEISLRPAQSFLMNKNQVPKLQPQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEKPAKTSKKPPPSKEELLKLTETVVTEYLNSGNANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYNSNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD DIP protein P78344-1 original sequence version: 121 eif4g2-eif4a1-4 3 7 unspecified role unspecified role prey prey ecolx Escherichia coli 8 unspecified role unspecified role bait bait P78344-1:p.Asn86Ala N86A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N86A'. Wed May 02 18:00:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn86ala' Tue Jun 30 19:56:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn86Ala' P78344-1:p.Glu125Lys E125K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E125K'. Wed May 02 18:56:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu125lys' Tue Jun 30 19:33:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu125Lys' Region 48-323 Region 48-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P78344-1:p.Phe296Ala F296A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F296A'. Wed May 02 14:34:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe296ala' Tue Jun 30 16:04:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe296Ala' His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction eif4a1-eif4g1-6 4 6 unspecified role unspecified role unspecified role unspecified role Region 732-1003 Region 732-1003 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction eif4a1-eif4g2-1 2 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role P78344-1:p.Asn86Ala N86A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain N A This feature has been corrected as a result of our quality control procedures. The original label was 'N86A'. Wed May 02 15:44:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn86ala' Tue Jun 30 17:37:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn86Ala' P78344-1:p.Glu125Lys E125K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E125K'. Wed May 02 19:56:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu125lys' Tue Jun 30 17:27:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu125Lys' Region 48-323 Region 48-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P78344-1:p.Phe296Ala F296A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F296A'. Wed May 02 14:50:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe296ala' Tue Jun 30 17:37:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe296Ala' ecolx Escherichia coli direct interaction direct interaction eif4a1-eif4g1-7 5 6 unspecified role unspecified role unspecified role unspecified role Region 732-1003 Region 732-1003 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction eif4a1-eif4g2-2 1 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 48-323 Region 48-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction