DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Science (New York, N.Y.) 2013 imex curation Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264959X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Science (New York, N.Y.) 2013 imex curation Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264956X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Science (New York, N.Y.) 2013 imex curation Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264958X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Science (New York, N.Y.) 2013 imex curation Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264954X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Science (New York, N.Y.) 2013 imex curation Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264957X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. Science (New York, N.Y.) 2013 imex curation Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Huang X., McGann JC., Liu BY., Hannoush RN., Lill JR., Pham V., Newton K., Kakunda M., Liu J., Yu C., Hymowitz SG., Hongo JA., Wynshaw-Boris A., Polakis P., Harland RM., Dixit VM. DIP original experiment: DIP-264955X Science (New York, N.Y.) 2013 IMEx Phosphorylation of Dishevelled by protein kinase RIPK4 regulates Wnt signaling. kpcd_human Protein kinase C delta type nPKC-delta PRKCD Tyrosine-protein kinase PRKCD PKCD protein protein human Homo sapiens Human MAPFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLCGINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED DIP protein Q05655 original sequence version: 92 DIP protein Q05655 original sequence version: 171 013F4314A2EE331A dvl2_human Segment polarity protein dishevelled homolog DVL-2 DSH homolog 2 DVL2 protein protein human Homo sapiens Human MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGTFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHTDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESEPSSRGGSLRRGGEASGTSDGGPPPSRGSTGGAPNLRAHPGLHPYGPPPGMALPYNPMMVVMMPPPPPPVPPAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM DIP protein O14641 original sequence version: 150 DIP protein O14641 original sequence version: 113 DIP protein O14641 original sequence version: 124 DIP protein O14641 original sequence version: 154 DIP protein O14641 original sequence version: 159 4BAD95B6C3FE531B p05771-1 Protein kinase C beta type PKCB PRKCB1 PRKCB1 PRKCB protein protein human Homo sapiens Human MADPAAGPPPSEGEESTVRFARKGALRQKNVHEVKNHKFTARFFKQPTFCSHCTDFIWGFGKQGFQCQVCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIQAHIDRDVLIVLVRDAKNLVPMDPNGLSDPYVKLKLIPDPKSESKQKTKTIKCSLNPEWNETFRFQLKESDKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKASVDGWFKLLSQEEGEYFNVPVPPEGSEANEELRQKFERAKISQGTKVPEEKTTNTVSKFDNNGNRDRMKLTDFNFLMVLGKGSFGKVMLSERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALPGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYHIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPGKRLGCGPEGERDIKEHAFFRYIDWEKLERKEIQPPYKPKARDKRDTSNFDKEFTRQPVELTPTDKLFIMNLDQNEFAGFSYTNPEFVINV DIP protein P05771-1 original sequence version: 141 DIP protein P05771 original sequence version: 196 3937E7B667108C68 lrp6_human Low-density lipoprotein receptor-related protein 6 LRP6 protein protein human Homo sapiens Human MGAVLRSLLACSFCVLLRAAPLLLYANRRDLRLVDATNGKENATIVVGGLEDAAAVDFVFSHGLIYWSDVSEEAIKRTEFNKTESVQNVVVSGLLSPDGLACDWLGEKLYWTDSETNRIEVSNLDGSLRKVLFWQELDQPRAIALDPSSGFMYWTDWGEVPKIERAGMDGSSRFIIINSEIYWPNGLTLDYEEQKLYWADAKLNFIHKSNLDGTNRQAVVKGSLPHPFALTLFEDILYWTDWSTHSILACNKYTGEGLREIHSDIFSPMDIHAFSQQRQPNATNPCGIDNGGCSHLCLMSPVKPFYQCACPTGVKLLENGKTCKDGATELLLLARRTDLRRISLDTPDFTDIVLQLEDIRHAIAIDYDPVEGYIYWTDDEVRAIRRSFIDGSGSQFVVTAQIAHPDGIAVDWVARNLYWTDTGTDRIEVTRLNGTMRKILISEDLEEPRAIVLDPMVGYMYWTDWGEIPKIERAALDGSDRVVLVNTSLGWPNGLALDYDEGKIYWGDAKTDKIEVMNTDGTGRRVLVEDKIPHIFGFTLLGDYVYWTDWQRRSIERVHKRSAEREVIIDQLPDLMGLKATNVHRVIGSNPCAEENGGCSHLCLYRPQGLRCACPIGFELISDMKTCIVPEAFLLFSRRADIRRISLETNNNNVAIPLTGVKEASALDFDVTDNRIYWTDISLKTISRAFMNGSALEHVVEFGLDYPEGMAVDWLGKNLYWADTGTNRIEVSKLDGQHRQVLVWKDLDSPRALALDPAEGFMYWTEWGGKPKIDRAAMDGSERTTLVPNVGRANGLTIDYAKRRLYWTDLDTNLIESSNMLGLNREVIADDLPHPFGLTQYQDYIYWTDWSRRSIERANKTSGQNRTIIQGHLDYVMDILVFHSSRQSGWNECASSNGHCSHLCLAVPVGGFVCGCPAHYSLNADNRTCSAPTTFLLFSQKSAINRMVIDEQQSPDIILPIHSLRNVRAIDYDPLDKQLYWIDSRQNMIRKAQEDGSQGFTVVVSSVPSQNLEIQPYDLSIDIYSRYIYWTCEATNVINVTRLDGRSVGVVLKGEQDRPRAVVVNPEKGYMYFTNLQERSPKIERAALDGTEREVLFFSGLSKPIALALDSRLGKLFWADSDLRRIESSDLSGANRIVLEDSNILQPVGLTVFENWLYWIDKQQQMIEKIDMTGREGRTKVQARIAQLSDIHAVKELNLQEYRQHPCAQDNGGCSHICLVKGDGTTRCSCPMHLVLLQDELSCGEPPTCSPQQFTCFTGEIDCIPVAWRCDGFTECEDHSDELNCPVCSESQFQCASGQCIDGALRCNGDANCQDKSDEKNCEVLCLIDQFRCANGQCIGKHKKCDHNVDCSDKSDELDCYPTEEPAPQATNTVGSVIGVIVTIFVSGTVYFICQRMLCPRMKGDGETMTNDYVVHGPASVPLGYVPHPSSLSGSLPGMSRGKSMISSLSIMGGSSGPPYDRAHVTGASSSSSSSTKGTYFPAILNPPPSPATERSHYTMEFGYSSNSPSTHRSYSYRPYSYRHFAPPTTPCSTDVCDSDYAPSRRMTSVATAKGYTSDLNYDSEPVPPPPTPRSQYLSAEENYESCPPSPYTERSYSHHLYPPPPSPCTDSS DIP protein O75581 original sequence version: 116 DIP protein O75581 original sequence version: 95 DIP protein O75581 original sequence version: 131 DIP protein O75581 original sequence version: 112 DIP protein O75581 original sequence version: 136 DIP protein O75581 original sequence version: 59 DIP protein O75581 original sequence version: 103 DIP protein O75581 original sequence version: 100 413D2CF70A5D8B5C ripk4_human Receptor-interacting serine/threonine-protein kinase 4 RIPK4 ANKRD3 DIK Ankyrin repeat domain-containing protein 3 PKC-delta-interacting protein kinase protein protein human Homo sapiens Human MEGDGGTPWALALLRTFDAGEFTGWEKVGSGGFGQVYKVRHVHWKTWLAIKCSPSLHVDDRERMELLEEAKKMEMAKFRYILPVYGICREPVGLVMEYMETGSLEKLLASEPLPWDLRFRIIHETAVGMNFLHCMAPPLLHLDLKPANILLDAHYHVKISDFGLAKCNGLSHSHDLSMDGLFGTIAYLPPERIREKSRLFDTKHDVYSFAIVIWGVLTQKKPFADEKNILHIMVKVVKGHRPELPPVCRARPRACSHLIRLMQRCWQGDPRVRPTFQGNGLNGELIRQVLAALLPVTGRWRSPGEGFRLESEVIIRVTCPLSSPQEITSETEDLCEKPDDEVKETAHDLDVKSPPEPRSEVVPARLKRASAPTFDNDYSLSELLSQLDSGVSQAVEGPEELSRSSSESKLPSSGSGKRLSGVSSVDSAFSSRGSLSLSFEREPSTSDLGTTDVQKKKLVDAIVSGDTSKLMKILQPQDVDLALDSGASLLHLAVEAGQEECAKWLLLNNANPNLSNRRGSTPLHMAVERRVRGVVELLLARKISVNAKDEDQWTALHFAAQNGDESSTRLLLEKNASVNEVDFEGRTPMHVACQHGQENIVRILLRRGVDVSLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCSDVNVCSLLAQTPLHVAAETGHTSTARLLLHRGAGKEAMTSDGYTALHLAARNGHLATVKLLVEEKADVLARGPLNQTALHLAAAHGHSEVVEELVSADVIDLFDEQGLSALHLAAQGRHAQTVETLLRHGAHINLQSLKFQGGHGPAATLLRRSKT DIP protein P57078 original sequence version: 134 5D8FFFD5F04F7ECB ripk4-prkcb-1 3 9 unspecified role unspecified role prey prey human Homo sapiens Human 7 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human association association dvl2-ripk4-1 4 11 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association dvl2-ripk4-2 5 11 unspecified role unspecified role unspecified role unspecified role Region 2-300 Region 2-300 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role Region 267-353 Region 267-353 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 436-505 Region 436-505 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O14641:p.Ser298Ala S298A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S298A'. Wed May 02 15:42:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser298ala' Tue Jun 30 18:11:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser298Ala' O14641:p.Ser480Ala S480A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S480A'. Wed May 02 18:11:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser480ala' Tue Jun 30 19:02:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser480Ala' in vitro In vitro phosphorylation phosphorylation reaction phosphorylation lrp6-ripk4-1 6 10 unspecified role unspecified role bait bait human Homo sapiens Human 11 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association dvl2-ripk4-3 1 11 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range in vitro In vitro 8 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range in vitro In vitro physical association physical association dvl2-ripk4-4 2 11 unspecified role unspecified role unspecified role unspecified role Region 2-300 Region 2-300 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P57078:p.Lys51Arg K51R mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K R This feature has been corrected as a result of our quality control procedures. The original label was 'K51R'. Wed May 02 15:04:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys51arg' Tue Jun 30 19:22:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys51Arg' spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 8 unspecified role unspecified role unspecified role unspecified role Region 267-353 Region 267-353 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 436-505 Region 436-505 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro phosphorylation phosphorylation reaction phosphorylation