DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252917X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252925X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252923X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252922X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252937X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252924X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions bacsu Bacillus subtilis crosslink cross-linking study crosslink predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252934X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252932X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252921X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252919X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252931X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252928X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252936X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252929X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252938X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252920X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions bacsu Bacillus subtilis crosslink cross-linking study crosslink predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252935X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252918X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252927X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252930X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions bacsu Bacillus subtilis crosslink cross-linking study crosslink predetermined predetermined participant predetermined Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252933X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. An asymmetric SMC-kleisin bridge in prokaryotic condensin. Nature structural & molecular biology 2013 imex curation Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Bürmann F., Shin HC., Basquin J., Soh YM., Giménez-Oya V., Kim YG., Oh BH., Gruber S. DIP original experiment: DIP-252926X Nature structural & molecular biology 2013 IMEx An asymmetric SMC-kleisin bridge in prokaryotic condensin. scpb_bacsu Segregation and condensation protein B ypuH scpB BSU23210 protein protein bacsu Bacillus subtilis MGLDIVNWKAIVEALLYAAGDEGLTKKQLLTVLEIEEPELNTIMADVADEYRGDTRGIELIEYADTYMLSTKKDFAPYLKKLIEVPSKGLSQASLEVLAIVSYKQPITRAEIEEIRGVKSERILHSLVAKALLCEVGRADGPGRAILYGTTPTFLEQFGLKTLDELPPLPENAEEDVLQEEADLFFENFNQTFEDIK DIP protein P35155 original sequence version: 92 77D27E0040A9D6F1 scpb_strr6 Segregation and condensation protein B scpB spr1690 protein protein strr6 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQKYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQPITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHLEELPVIDELEIQAQESQLFGERIEEDENQ DIP protein Q8DNI9 original sequence version: 69 smc_bacsu Chromosome partition protein Smc smc ylqA BSU15940 protein protein bacsu Bacillus subtilis MFLKRLDVIGFKSFAERISVDFVKGVTAVVGPNGSGKSNITDAIRWVLGEQSARSLRGGKMEDIIFAGSDSRKRLNLAEVTLTLDNDDHFLPIDFHEVSVTRRVYRSGESEFLINNQPCRLKDIIDLFMDSGLGKEAFSIISQGKVEEILSSKAEDRRSIFEEAAGVLKYKTRKKKAENKLFETQDNLNRVEDILHELEGQVEPLKIQASIAKDYLEKKKELEHVEIALTAYDIEELHGKWSTLKEKVQMAKEEELAESSAISAKEAKIEDTRDKIQALDESVDELQQVLLVTSEELEKLEGRKEVLKERKKNAVQNQEQLEEAIVQFQQKETVLKEELSKQEAVFETLQAEVKQLRAQVKEKQQALSLHNENVEEKIEQLKSDYFELLNSQASIRNELQLLDDQMSQSAVTLQRLADNNEKHLQERHDISARKAACETEFARIEQEIHSQVGAYRDMQTKYEQKKRQYEKNESALYQAYQYVQQARSKKDMLETMQGDFSGFYQGVKEVLKAKERLGGIRGAVLELISTEQKYETAIEIALGASAQHVVTDDEQSARKAIQYLKQNSFGRATFLPLSVIRDRQLQSRDAETAARHSSFLGVASELVTFDPAYRSVIQNLLGTVLITEDLKGANELAKLLGHRYRIVTLEGDVVNPGGSMTGGAVKKKNNSLLGRSRELEDVTKRLAEMEEKTALLEQEVKTLKHSIQDMEKKLADLRETGEGLRLKQQDVKGQLYELQVAEKNINTHLELYDQEKSALSESDEERKVRKRKLEEELSAVSEKMKQLEEDIDRLTKQKQTQSSTKESLSNELTELKIAAAKKEQACKGEEDNLARLKKELTETELALKEAKEDLSFLTSEMSSSTSGEEKLEEAAKHKLNDKTKTIELIALRRDQRIKLQHGLDTYERELKEMKRLYKQKTTLLKDEEVKLGRMEVELDNLLQYLREEYSLSFEGAKEKYQLETDPEEARKRVKLIKLAIEELGTVNLGSIDEFERVNERYKFLSEQKEDLTEAKNTLFQVIEEMDEEMTKRFNDTFVQIRSHFDQVFRSLFGGGRAELRLTDPNDLLHSGVEIIAQPPGKKLQNLNLLSGGERALTAIALLFSILKVRPVPFCVLDEVEAALDEANVFRFAQYLKKYSSDTQFIVITHRKGTMEEADVLYGVTMQESGVSKVISVKLEETKEFVQ DIP protein P51834 original sequence version: 94 ACC5D1A170453212 smc_pyrfu Chromosome partition protein Smc PF1843 smc protein protein pyrfu Pyrococcus furiosus MPYIEKLELKGFKSYGNKKVVIPFSKGFTAIVGANGSGKSNIGDAILFVLGGLSAKAMRASRISDLIFAGSKNEPPAKYAEVAIYFNNEDRGFPIDEDEVVIRRRVYPDGRSSYWLNGRRATRSEILDILTAAMISPDGYNIVLQGDITKFIKMSPLERRLLIDDISGIAEYDSKKEKALEELKQAEENLARVDLLIKEVKKQLDKLEKERNDALRYLDLKDKLEKAKVSLLLGEIKILETQIKEGEKRRAEIEEEIQKIEKEIEKIGKEIVEKVKVLREIEERIEKESGEEAIQITKKIGEVTSKIELTKRNIEVAKEELEDAQRRLAKTKEELRKVLSEIEKSKGAITRWKKRRDALINEIKKKEEERNVLVVKLGEIDKTFGAAREEFDSVVKELEETTRKMYEIEGNIRRLQEEKEKLHSRILFLRAKLPGIKEKINEFKAVVEDKRAEISEIEGKLSTIQAKRIKVEKEIEAKSNELEKVSKELESSERELIAAEAQREVRGNRAAEELKRSGIGGIYGTLAELIKVKDEAYALAIEVALGNRADNVVVEDELVAEKAIKYLKEHKLGRLTFLPLNKIKPKHVDSSVGLPAVDVIEYDQKIENAVKFALGDTVIVNSMEEARPHIGKVRMVTIEGELYERSGAITGGHFRARGLAVDTTKLREKVESLRRRKEALEGELNSLKIELRSLENASFELRIKLSDEKKELELASKDLNRLLEEENAVKEEIEESERKIQEIEQKIENEKSELAKLRGRIQRLERKKEKLKKALENPEARELMEKIRIIDGEISSLKEELSRIESRIESLESRLNEELLPRKASLEEEIEGLVNKINALKNNISENEKALELLNKELEKLKSIEENIKGEIRTLREKRKKLEEDISKLREKKEVLQRKLQELEIEANTLKVRDAQLNAQLEEKKYQLTHYDKNLIKSIKEIPLDLEKVKKEIEKMEEEIRSLEPVNMKAIEDFEIVERRYLELKSKREKLEAEKESIIEFINEIEKEKKNVFMRTFEAISRNFSEIFAKLSPGGSARLILENPEDPFSGGLEIEAKPAGKDVKRIEAMSGGEKALTALAFVFAIQKFKPAPFYLFDEIDAHLDDANVKRVADLIKESSKESQFIVITLRDVMMANADKIIGVSMRDGVSKVVSLSLEKAMKILEEIRKKQGWEHGN DIP protein Q8TZY2 original sequence version: 70 EC12C4C060E5A1CB scpa_strr6 Segregation and condensation protein A scpA spr1691 protein protein strr6 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVSTLQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLLGEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHTTILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELILVQEESFGDIYLMEKKEESQVPQS DIP protein Q9EUQ7 original sequence version: 58 q8tzy3_pyrfu PF1842 protein protein pyrfu Pyrococcus furiosus MEIEVTPVDILLQLVKMGKVDPWNIDIVDLTEKYIKMLREMQELDLRISARAILAAAILVRMKSEALLNEDSREQEEKEEERIRVEVDPLVPPLRRVERYYTLDDLIDALMEALEEAERRKPKKKKKVEIDEEIFVIDDFRVDIEKYVEELYKVVKKIYEKTGTPIKFWDLVPDVEPKIIARTFLYLLFLENMGRVEIIQEEPFGEILVVPM DIP protein Q8TZY3 original sequence version: 44 scpa_bacsu Segregation and condensation protein A ypuG scpA BSU23220 protein protein bacsu Bacillus subtilis MEEYQVKIDTFEGPLDLLLHLINRLEIDIYDIPVAKITEQYLLYVHTMRVLELDIASEYLVMAATLLSIKSRMLLPKQEEELFEDELLEEEDPREELIEKLIEYRKYKDAAKDLKEREEERQKSFTKPPSDLSEYAKEVKQSEQKLSVTVYDMIGAFQKVLKRKKINRPMETTITRQDIPIEARMNEIVHSLKSRGTRINFMDLFPYEQKEHLVVTFLAVLELMKNQLVLIEQEHNFSDIYITGSESIHGA DIP protein P35154 original sequence version: 85 9FE03F117D2919A0 scpa-smc-5 2 29 unspecified role unspecified role bait bait Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 25 unspecified role unspecified role prey prey Region 161-219/983-1037 Region 161-219/983-1037 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association q8tzy3_pyrfu-smc-1 1 28 unspecified role unspecified role unspecified role unspecified role Region 126-212 Region 126-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 26 unspecified role unspecified role unspecified role unspecified role Region 1-182/1006-1177 Region 1-182/1006-1177 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction scpa-scpb-5 9 24 unspecified role unspecified role unspecified role unspecified role Region 1-183 Region 1-183 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 27 unspecified role unspecified role unspecified role unspecified role Region 1-160 Region 1-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction scpa-smc-6 12 29 unspecified role unspecified role unspecified role unspecified role Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 25 unspecified role unspecified role unspecified role unspecified role Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction q8tzy3_pyrfu-smc-2 8 28 unspecified role unspecified role unspecified role unspecified role Region 126-212 Region 126-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 26 unspecified role unspecified role unspecified role unspecified role Region 1-182/1006-1177 Region 1-182/1006-1177 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction scpa-scpa-1 13 29 unspecified role unspecified role unspecified role unspecified role Region 148-251 Region 148-251 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 29 unspecified role unspecified role unspecified role unspecified role Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 25 unspecified role unspecified role unspecified role unspecified role Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association scpa-scpb-6 11 23 unspecified role unspecified role prey prey ecolx Escherichia coli 29 unspecified role unspecified role bait bait Region 1-168 Region 1-168 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P35154:p.Leu60Lys L60K mutation mutation certain certain sequence position certain certain certain sequence position certain L K This feature has been corrected as a result of our quality control procedures. The original label was 'L60K'. Wed May 02 16:56:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu60lys' Tue Jun 30 19:04:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu60Lys' P35154:p.Leu67Glu L67E mutation mutation certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L67E'. Wed May 02 19:09:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu67glu' Tue Jun 30 16:18:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu67Glu' P35154:p.Lys70Glu K70E mutation mutation certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K70E'. Wed May 02 15:19:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys70glu' Tue Jun 30 18:12:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys70Glu' ecolx Escherichia coli physical association physical association scpa-scpa-2 15 29 unspecified role unspecified role unspecified role unspecified role Region 148-251 Region 148-251 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 29 unspecified role unspecified role unspecified role unspecified role Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 25 unspecified role unspecified role unspecified role unspecified role Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association q8tzy3_pyrfu-smc-3 18 28 unspecified role unspecified role unspecified role unspecified role Region 126-212 Region 126-212 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 26 unspecified role unspecified role unspecified role unspecified role Region 1-182/1006-1177 Region 1-182/1006-1177 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction scpa-smc-7 22 29 unspecified role unspecified role unspecified role unspecified role Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 25 unspecified role unspecified role unspecified role unspecified role Region 161-219/983-1037 Region 161-219/983-1037 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction scpb-scpa-1 4 24 unspecified role unspecified role bait bait Region 1-183 Region 1-183 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 27 unspecified role unspecified role prey prey Region 1-160 Region 1-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association scpa-scpb-7 14 23 unspecified role unspecified role prey prey ecolx Escherichia coli 29 unspecified role unspecified role bait bait Region 1-168 Region 1-168 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 25 unspecified role unspecified role prey prey Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli association association scpa-smc-8 17 29 unspecified role unspecified role unspecified role unspecified role P35154:p.[Glu52Cys;His235Cys] E52C/H235C mutation mutation certain certain sequence position certain certain certain sequence position certain E C certain certain sequence position certain certain certain sequence position certain H C Wed May 02 16:37:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu52cys,his235cys' Tue Jun 30 19:11:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu52Cys;His235Cys]' bacsu Bacillus subtilis 25 unspecified role unspecified role unspecified role unspecified role P51834:p.[Ser19Cys;Arg1032Cys] mutation mutation certain certain sequence position certain certain certain sequence position certain S C certain certain sequence position certain certain certain sequence position certain R C Fri Jul 28 10:50:17 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser19cys,ser1032cys' Wed May 02 15:41:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser19cys,arg1032cys' Tue Jun 30 18:23:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser19Cys;Arg1032Cys]' bacsu Bacillus subtilis direct interaction direct interaction scpa-scpb-8 10 24 unspecified role unspecified role unspecified role unspecified role Region 1-183 Region 1-183 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 27 unspecified role unspecified role unspecified role unspecified role Region 1-160 Region 1-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction scpa-smc-9 3 29 unspecified role unspecified role unspecified role unspecified role Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 25 unspecified role unspecified role unspecified role unspecified role Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction smc-scpa-1 19 29 unspecified role unspecified role prey prey Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 25 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain P51834:p.Val1021Glu V1021E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V E This feature has been corrected as a result of our quality control procedures. The original label was 'V1021E'. Wed May 02 17:02:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val1021glu' Tue Jun 30 17:42:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val1021Glu' P51834:p.Tyr170Glu Y170E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y E This feature has been corrected as a result of our quality control procedures. The original label was 'Y170E'. Wed May 02 18:05:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr170glu' Tue Jun 30 16:38:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr170Glu' ecolx Escherichia coli physical association physical association scpa-smc-10 20 29 unspecified role unspecified role bait bait Region 1-168 Region 1-168 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P35154:p.Leu60Lys L60K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L K This feature has been corrected as a result of our quality control procedures. The original label was 'L60K'. Wed May 02 17:32:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu60lys' Tue Jun 30 17:52:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu60Lys' P35154:p.Leu67Glu L67E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L67E'. Wed May 02 15:09:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu67glu' Tue Jun 30 19:28:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu67Glu' P35154:p.Lys70Glu K70E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K E This feature has been corrected as a result of our quality control procedures. The original label was 'K70E'. Wed May 02 18:43:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys70glu' Tue Jun 30 17:48:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys70Glu' ecolx Escherichia coli 25 unspecified role unspecified role prey prey Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association scpa-scpa-3 5 29 unspecified role unspecified role bait bait Region 148-251 Region 148-251 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 29 unspecified role unspecified role prey prey Region 1-86 Region 1-86 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 25 unspecified role unspecified role prey prey Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli association association scpa-smc-11 7 29 unspecified role unspecified role unspecified role unspecified role P35154:p.Glu52Cys E52C mutation mutation certain certain sequence position certain certain certain sequence position certain E C This feature has been corrected as a result of our quality control procedures. The original label was 'E52C'. Wed May 02 19:27:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu52cys' Tue Jun 30 17:25:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu52Cys' bacsu Bacillus subtilis 25 unspecified role unspecified role unspecified role unspecified role P51834:p.Arg1032Cys R1032C mutation mutation certain certain sequence position certain certain certain sequence position certain R C This feature has been corrected as a result of our quality control procedures. The original label was 'R1032C'. Wed May 02 15:26:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1032cys' Tue Jun 30 17:31:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1032Cys' bacsu Bacillus subtilis direct interaction direct interaction scpa-scpb-9 16 24 unspecified role unspecified role unspecified role unspecified role Region 1-183 Region 1-183 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 27 unspecified role unspecified role unspecified role unspecified role Region 1-160 Region 1-160 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction smc-scpa-2 6 29 unspecified role unspecified role prey prey Region 148-251 Region 148-251 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 25 unspecified role unspecified role bait bait Region 1-219/983-1186 Region 1-219/983-1186 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P51834:p.Ile1174Glu I1174E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I E This feature has been corrected as a result of our quality control procedures. The original label was 'I1174E'. Wed May 02 15:07:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile1174glu' Tue Jun 30 17:08:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile1174Glu' P51834:p.Val1176Glu V1176E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V E This feature has been corrected as a result of our quality control procedures. The original label was 'V1176E'. Wed May 02 18:28:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val1176glu' Tue Jun 30 17:20:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val1176Glu' ecolx Escherichia coli physical association physical association scpa-smc-12 21 29 unspecified role unspecified role unspecified role unspecified role P35154:p.His235Cys H235C mutation mutation certain certain sequence position certain certain certain sequence position certain H C This feature has been corrected as a result of our quality control procedures. The original label was 'H235C'. Wed May 02 15:57:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his235cys' Tue Jun 30 18:04:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His235Cys' bacsu Bacillus subtilis 25 unspecified role unspecified role unspecified role unspecified role P51834:p.Ser19Cys S19C mutation mutation certain certain sequence position certain certain certain sequence position certain S C This feature has been corrected as a result of our quality control procedures. The original label was 'S19C'. Wed May 02 18:02:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser19cys' Tue Jun 30 20:01:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser19Cys' bacsu Bacillus subtilis direct interaction direct interaction