DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Sequential primed kinases create a damage-responsive phosphodegron on Eco1. Sequential primed kinases create a damage-responsive phosphodegron on Eco1. Nature structural & molecular biology 2013 imex curation Lyons NA., Fonslow BR., Diedrich JK., Yates JR., Morgan DO. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Lyons NA., Fonslow BR., Diedrich JK., Yates JR., Morgan DO. DIP original experiment: DIP-251986X Nature structural & molecular biology 2013 IMEx Sequential primed kinases create a damage-responsive phosphodegron on Eco1. mck1_yeast Protein kinase MCK1 YPK1 YNL307C N0392 MCK1 Meiosis and centromere regulatory kinase protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTEEQNGVPLQRGSEFIADDVTSNKSNNTRRMLVKEYRKIGRGAFGTVVQAYLTQDKKNWLGPFAIKKVPAHTEYKSRELQILRIADHPNIVKLQYFFTHLSPQDNKVYQHLAMECLPETLQIEINRYVTNKLEMPLKHIRLYTYQIARGMLYLHGLGVCHRDIKPSNVLVDPETGVLKICDFGSAKKLEHNQPSISYICSRFYRAPELIIGCTQYTTQIDIWGLGCVMGEMLIGKAIFQGQEPLLQLREIAKLLGPPDKRFIFFSNPAYDGPLFSKPLFSGSSQQRFEKYFGHSGPDGIDLLMKILVYEPQQRLSPRRILAHQFFNELRNDDTFLPRGFTEPIKLPNLFDFNDFELQILGEFADKIKPTKVAE BCF0C776B6E3841B BCF0C776B6E3841B eco1_yeast N-acetyltransferase ECO1 ECO1 CTF7 YFR027W Establishment of cohesion protein 1 Chromosome transmission fidelity protein 7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKARKSQRKAGSKPNLIQSKLQVNNGSKSNKIVKCDKCEMSYSSTSIEDRAIHEKYHTLQLHGRKWSPNWGSIVYTERNHSRTVHLSRSTGTITPLNSSPLKKSSPSITHQEEKIVYVRPDKSNGEVRAMTEIMTLVNNELNAPHDENVIWNSTTEEKGKAFVYIRNDRAVGIIIIENLYGGNGKTSSRGRWMVYDSRRLVQNVYPDFKIGISRIWVCRTARKLGIATKLIDVARENIVYGEVIPRYQVAWSQPTDSGGKLASKYNGIMHKSGKLLLPVYI 0AF1B59BE9E71818 0AF1B59BE9E71818 eco1-mck1-1 1 3 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 91-103 Region 91-103 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pS98 pS98 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain prerequisite-ptm prerequisite-ptm prerequisite ptm required ptm prerequisite-ptm required-ptm P43605:p.Thr94Ala T94A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T94A'. Wed May 02 15:03:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr94ala' Tue Jun 30 18:42:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr94Ala' in vitro In vitro 2 enzyme enzyme unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast phosphorylation phosphorylation reaction phosphorylation