DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. Nature 2013 imex curation Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252040X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. Nature 2013 imex curation Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252037X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. Nature 2013 imex curation Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252035X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. Nature 2013 imex curation Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252036X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. Nature 2013 imex curation Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252038X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. How insulin engages its primary binding site on the insulin receptor. Nature 2013 imex curation Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Menting JG., Whittaker J., Margetts MB., Whittaker LJ., Kong GK., Smith BJ., Watson CJ., Záková L., Kletvíková E., Jiráček J., Chan SJ., Steiner DF., Dodson GG., Brzozowski AM., Weiss MA., Ward CW., Lawrence MC. DIP original experiment: DIP-252039X Nature 2013 IMEx How insulin engages its primary binding site on the insulin receptor. ins_human Insulin INS protein protein human Homo sapiens Human MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN DIP protein P01308 original sequence version: 184 DIP protein P01308 original sequence version: 104 DIP protein P01308 original sequence version: 177 Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver mint homomint Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730] C2C3B23B85E520E5 p06213-2 Insulin receptor HIR-A INSR protein protein human Homo sapiens Human MATGGRRGAAAAPLLVAVAALLLGAAGHLYPGEVCPGMDIRNNLTRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDLFPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSVEDNYIVLNKDDNEECGDICPGTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHSECLGNCSQPDDPTKCVACRNFYLDGRCVETCPPPYYHFQDWRCVNFSFCQDLHHKCKNSRRQGCHQYVIHNNKCIPECPSGYTMNSSNLLCTPCLGPCPKVCHLLEGEKTIDSVTSAQELRGCTVINGSLIINIRGGNNLAAELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLTITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELLKFSYIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPLRSNDPKSQNHPGWLMRGLKPWTQYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVFWERQAEDSELFELDYCLKGLKLPSRTWSPPFESEDSQKHNQSEYEDSAGECCSCPKTDSQILKELEESSFRKTFEDYLHNVVFVPRPSRKRRSLGDVGNVTVAVPTVAAFPNTSSTSVPTSPEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRIRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSVVIGSIYLFLRKRQPDGPLGPLYASSNPEYLSASDVFPCSVYVPDEWEVSREKITLLRELGQGSFGMVYEGNARDIIKGEAETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSYLRSLRPEAENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDQPDNCPERVTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFHSEENKAPESEELEMEFEDMENVPLDRSSHCQREEAGGRDGGSSLGFKRSYEEHIPYTHMNGGKKNGRILTLPRSNPS DIP protein P06213-2 original sequence version: 186 A396F39279C2764D ins_bovin Insulin INS protein protein bovin Bos taurus (Bovine) Bovine MALWTRLRPLLALLALWPPPPARAFVNQHLCGSHLVEALYLVCGERGFFYTPKARREVEGPQVGALELAGGPGAGGLEGPPQKRGIVEQCCASVCSLYQLENYCN DIP protein P01317 original sequence version: 131 75307CF78E61C06A ins-insr-1 3 7 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 90-110 Region 90-110 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role unspecified role unspecified role Region 28-337 Region 28-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 731-746 Region 731-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction insr-7 1 8 unspecified role unspecified role unspecified role unspecified role Region 28-337 Region 28-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction ins-insr-2 2 7 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 90-110 Region 90-110 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role Region 28-337 Region 28-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 724-746 Region 724-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction ins-insr-3 6 9 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 85-105 Region 85-105 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain bovin Bos taurus (Bovine) Bovine 8 unspecified role unspecified role unspecified role unspecified role Region 28-620/731-746 Region 28-620/731-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction ins-insr-4 4 7 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 90-110 Region 90-110 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 8 unspecified role unspecified role unspecified role unspecified role Region 28-337 Region 28-337 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 731-746 Region 731-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction ins-insr-5 5 9 unspecified role unspecified role unspecified role unspecified role Region 25-54 Region 25-54 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Region 85-105 Region 85-105 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain bovin Bos taurus (Bovine) Bovine 8 unspecified role unspecified role unspecified role unspecified role Region 28-620/731-746 Region 28-620/731-746 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction