DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Nature structural & molecular biology 2013 imex curation Rangarajan ES., Izard T. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Rangarajan ES., Izard T. DIP original experiment: DIP-251984X Nature structural & molecular biology 2013 IMEx Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Nature structural & molecular biology 2013 imex curation Rangarajan ES., Izard T. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Rangarajan ES., Izard T. DIP original experiment: DIP-251983X Nature structural & molecular biology 2013 IMEx Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Nature structural & molecular biology 2013 imex curation Rangarajan ES., Izard T. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Rangarajan ES., Izard T. DIP original experiment: DIP-251981X Nature structural & molecular biology 2013 IMEx Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Nature structural & molecular biology 2013 imex curation Rangarajan ES., Izard T. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Rangarajan ES., Izard T. DIP original experiment: DIP-251985X Nature structural & molecular biology 2013 IMEx Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Dimer asymmetry defines α-catenin interactions. Nature structural & molecular biology 2013 imex curation Rangarajan ES., Izard T. Only protein-protein interactions ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Rangarajan ES., Izard T. DIP original experiment: DIP-251982X Nature structural & molecular biology 2013 IMEx Dimer asymmetry defines α-catenin interactions. p18206-2 Vinculin VCL Metavinculin Vinculin protein protein human Homo sapiens Human MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ DIP protein P18206-2 original sequence version: 120 DIP protein P18206-2 original sequence version: 152 C9B67AA072009EBD ctna1_human Catenin alpha-1 Cadherin-associated protein Alpha E-catenin Renal carcinoma antigen NY-REN-13 CTNNA1 protein protein human Homo sapiens Human MTAVHAGNINFKWDPKSLEIRTLAVERLLEPLVTQVTTLVNTNSKGPSNKKRGRSKKAHVLAASVEQATENFLEKGDKIAKESQFLKEELVAAVEDVRKQGDLMKAAAGEFADDPCSSVKRGNMVRAARALLSAVTRLLILADMADVYKLLVQLKVVEDGILKLRNAGNEQDLGIQYKALKPEVDKLNIMAAKRQQELKDVGHRDQMAAARGILQKNVPILYTASQACLQHPDVAAYKANRDLIYKQLQQAVTGISNAAQATASDDASQHQGGGGGELAYALNNFDKQIIVDPLSFSEERFRPSLEERLESIISGAALMADSSCTRDDRRERIVAECNAVRQALQDLLSEYMGNAGRKERSDALNSAIDKMTKKTRDLRRQLRKAVMDHVSDSFLETNVPLLVLIEAAKNGNEKEVKEYAQVFREHANKLIEVANLACSISNNEEGVKLVRMSASQLEALCPQVINAALALAAKPQSKLAQENMDLFKEQWEKQVRVLTDAVDDITSIDDFLAVSENHILEDVNKCVIALQEKDVDGLDRTAGAIRGRAARVIHVVTSEMDNYEPGVYTEKVLEATKLLSNTVMPRFTEQVEAAVEALSSDPAQPMDENEFIDASRLVYDGIRDIRKAVLMIRTPEELDDSDFETEDFDVRSRTSVQTEDDQLIAGQSARAIMAQLPQEQKAKIAEQVASFQEEKSKLDAEVSKWDDSGNDIIVLAKQMCMIMMEMTDFTRGKGPLKNTSDVISAAKKIAEAGSRMDKLGRTIADHCPDSACKQDLLAYLQRIALYCHQLNICSKVKAEVQNLGGELVVSGVDSAMSLIQAAKNLMNAVVQTVKASYVASTKYQKSQGMASLNLPAVSWKMKAPEKKPLVKREKQDETQTKIKRASQKKHVNPVQALSEFKAMDSI DIP protein P35221 original sequence version: 157 DIP protein P35221 original sequence version: 82 DIP protein P35221-1 original sequence version: 82 DIP protein P35221-1 original sequence version: 136 DIP protein P35221 original sequence version: 158 DIP protein P35221 original sequence version: 167 DIP protein P35221-1 original sequence version: 146 DIP protein P35221 original sequence version: 146 7AAE6F5DDBAF5099 ctnna1-1 3 7 unspecified role unspecified role unspecified role unspecified role Region 82-906 Region 82-906 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ctnna1-vcl-1 1 6 unspecified role unspecified role unspecified role unspecified role Region 1-840 Region 1-840 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 82-906 Region 82-906 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ctnna1-4 5 7 unspecified role unspecified role unspecified role unspecified role Region 82-906 Region 82-906 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ctnna1-vcl-2 4 6 unspecified role unspecified role unspecified role unspecified role Region 1-840 Region 1-840 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 82-906 Region 82-906 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ctnna1-5 2 7 unspecified role unspecified role unspecified role unspecified role Region 82-906 Region 82-906 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction