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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="pubmed" dbAc="MI:0446" id="14681454" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579232" refType="identity" refTypeAc="MI:0356"/>
      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="23273427" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="doi" dbAc="MI:0574" id="10.1016/j.str.2012.11.016" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16030346" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-20747" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.</attribute>
            <attribute name="journal" nameAc="MI:0885">Structure (London, England : 1993)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2013</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Alayyoubi M.,  Guo H.,  Dey S.,  Golnazarian T.,  Brooks GA.,  Rong A.,  Miller JF.,  Ghosh P.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-20747" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-251905X" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="wwpdb" dbAc="MI:0805" id="4DWL" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="23273427" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="doi" dbAc="MI:0574" id="10.1016/j.str.2012.11.016" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1272" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Alayyoubi M., Guo H., Dey S., Golnazarian T., Brooks GA., Rong A., Miller JF., Ghosh P.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-251905X</attribute>
          <attribute name="journal" nameAc="MI:0885">Structure (London, England : 1993)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2013</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.</attribute>
        </attributeList>
      </experimentDescription>
      <experimentDescription id="2">
        <names>
          <fullName>Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="23273427" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="doi" dbAc="MI:0574" id="10.1016/j.str.2012.11.016" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16030346" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-20747" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.</attribute>
            <attribute name="journal" nameAc="MI:0885">Structure (London, England : 1993)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2013</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Alayyoubi M.,  Guo H.,  Dey S.,  Golnazarian T.,  Brooks GA.,  Rong A.,  Miller JF.,  Ghosh P.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-20747" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-251906X" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="23273427" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="doi" dbAc="MI:0574" id="10.1016/j.str.2012.11.016" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>pull down</shortLabel>
            <fullName>pull down</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0096" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1223" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>weight by comassie</shortLabel>
            <fullName>molecular weight estimation by coomasie staining</fullName>
            <alias type="synonym" typeAc="MI:1041">weight by comassie</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0818" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1218596" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Alayyoubi M., Guo H., Dey S., Golnazarian T., Brooks GA., Rong A., Miller JF., Ghosh P.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-251906X</attribute>
          <attribute name="journal" nameAc="MI:0885">Structure (London, England : 1993)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2013</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Structure of the essential diversity-generating retroelement protein bAvd and its functionally important interaction with reverse transcriptase.</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="3">
        <names>
          <shortLabel>q775d8_bpbpp</shortLabel>
          <alias type="gene name" typeAc="MI:0301">brt</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q775D8" version="33" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16030391" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003964" version="TrEMBL_59"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0004519" version="TrEMBL_59"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000477" version="TrEMBL_59"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR043502" version="TrEMBL_59"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_958675.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-60104N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="194699">
          <names>
            <shortLabel>bpbpp</shortLabel>
            <fullName>Bordetella phage BPP-1</fullName>
          </names>
        </organism>
        <sequence>MGKRHRNLIDQITTWENLLDAYRKTSHGKRRTWGYLEFKEYDLANLLALQAELKAGNYERGPYREFLVYEPKPRLISALEFKDRLVQHALCNIVAPIFEAGLLPYTYACRPDKGTHAGVCHVQAELRRTRATHFLKSDFSKFFPSIDRAALYAMIDKKIHCAATRRLLRVVLPDEGVGIPIGSLTSQLFANVYGGAVDRLLHDELKQRHWARYMDDIVVLGDDPEELRAVFYRLRDFASERLGLKISHWQVAPVSRGINFLGYRIWPTHKLLRKSSVKRAKRKVANFIKHGEDESLQRFLASWSGHAQWADTHNLFTWMEEQYGIACH</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein Q775D8 original sequence version: 33</attribute>
        </attributeList>
      </interactor>
      <interactor id="4">
        <names>
          <shortLabel>q775d7_bpbpp</shortLabel>
          <alias type="gene name" typeAc="MI:0301">bbp7</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q775D7" version="20" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16030366" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4DWL" version="TrEMBL_47"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR036583" version="TrEMBL_47"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_958676.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-60103N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="194699">
          <names>
            <shortLabel>bpbpp</shortLabel>
            <fullName>Bordetella phage BPP-1</fullName>
          </names>
        </organism>
        <sequence>MEPIEEATKCYDQMLIVERYERVISYLYPIAQSIPRKHGVAREMFLKCLLGQVELFIVAGKSNQVSKLYAADAGLAMLRFWLRFLAGIQKPHAMTPHQVETAQVLIAEVGRILGSWIARVNRKGQAGK</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein Q775D7 original sequence version: 20</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="5" imexId="IM-20747-1">
        <names>
          <shortLabel>bbp7-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-190410E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16030375" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-20747-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="6">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
      <interaction id="7" imexId="IM-20747-2">
        <names>
          <shortLabel>brt-bbp7-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-190411E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-16030400" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-20747-2" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>2</experimentRef>
        </experimentList>
        <participantList>
          <participant id="8">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="9">
                <names>
                  <shortLabel>Q775D7:p.Arg79Ala</shortLabel>
                  <fullName>R79A</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-16030411" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting strength</shortLabel>
                    <fullName>mutation disrupting interaction strength</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting strength</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1128" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528001" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>alanine scanning</shortLabel>
                    <fullName>alanine scanning</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456759" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="79"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="79"/>
                    <resultingSequence>
                      <originalSequence>R</originalSequence>
                      <newSequence>A</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'R79A'.</attribute>
                  <attribute name="remark-internal">Wed May 02 19:11:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg79ala'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 16:18:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg79Ala'</attribute>
                </attributeList>
              </feature>
              <feature id="10">
                <names>
                  <shortLabel>Q775D7:p.Arg83Ala</shortLabel>
                  <fullName>R83A</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-16030414" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting strength</shortLabel>
                    <fullName>mutation disrupting interaction strength</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting strength</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1128" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528001" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>alanine scanning</shortLabel>
                    <fullName>alanine scanning</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456759" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="83"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="83"/>
                    <resultingSequence>
                      <originalSequence>R</originalSequence>
                      <newSequence>A</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'R83A'.</attribute>
                  <attribute name="remark-internal">Wed May 02 15:53:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg83ala'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 18:40:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg83Ala'</attribute>
                </attributeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
          <participant id="11">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>bait</shortLabel>
                  <fullName>bait</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0496" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-49" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="12">
                <names>
                  <shortLabel>Q775D8:p.Asp138Gln</shortLabel>
                  <fullName>D138Q</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-16030405" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation</shortLabel>
                    <fullName>mutation</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0118" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456558" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="138"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="138"/>
                    <resultingSequence>
                      <originalSequence>D</originalSequence>
                      <newSequence>Q</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'D138Q'.</attribute>
                  <attribute name="remark-internal">Wed May 02 16:32:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp138gln'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 19:41:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp138Gln'</attribute>
                </attributeList>
              </feature>
              <feature id="13">
                <names>
                  <shortLabel>His-tag</shortLabel>
                  <fullName>His-tag</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-16030408" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>his tag</shortLabel>
                    <fullName>his tag</fullName>
                    <alias type="go synonym" typeAc="MI:0303">Hexa-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">6-His-tag</alias>
                    <alias type="go synonym" typeAc="MI:0303">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Hexa-His-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">Histidine-tag</alias>
                    <alias type="synonym" typeAc="MI:1041">6-His-tag</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0521" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456516" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>n-term range</shortLabel>
                        <fullName>n-terminal range</fullName>
                        <alias type="synonym" typeAc="MI:1041">n-term range</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1040" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-2929795" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14760721" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <endStatus>
                      <names>
                        <shortLabel>n-term range</shortLabel>
                        <fullName>n-terminal range</fullName>
                        <alias type="synonym" typeAc="MI:1041">n-term range</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1040" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-2929795" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14760721" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                  </featureRange>
                </featureRangeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
    </interactionList>
  </entry>
</entrySet>