DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. Science (New York, N.Y.) 2012 imex curation Kohl KP., Jones CD., Sekelsky J. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kohl KP., Jones CD., Sekelsky J. DIP original experiment: DIP-268255X Science (New York, N.Y.) 2012 IMEx Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. Science (New York, N.Y.) 2012 imex curation Kohl KP., Jones CD., Sekelsky J. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kohl KP., Jones CD., Sekelsky J. DIP original experiment: DIP-268256X Science (New York, N.Y.) 2012 IMEx Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. Science (New York, N.Y.) 2012 imex curation Kohl KP., Jones CD., Sekelsky J. Only protein-protein interactions spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kohl KP., Jones CD., Sekelsky J. DIP original experiment: DIP-268257X Science (New York, N.Y.) 2012 IMEx Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase. q0khr4_drome mei-218 CG33067 CG8923 mei-218-RB mei-218-RD Dmel_CG8923 Dmel\CG8923 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSTNKLEKKDPRRHIPLQLNVFLRNDDDGEVREVTQNANTSSIGTIPSNGLVESDVTPIMTPTPKAGVYKPNVNVWRLSKEVTKPDRSFVVSAEEYPDLGQRPKRFKQRTPRVRNQDDGGTVSCLASRFSRVKDPPVLGNFLPDFMLPKSSNSNHQSVTTWLSSVRPSDSQKSLSDQARCSSSTNITLLSKDSVQPLTPKSSAQSSPGEKNIQQEQNTPAKSQQEETSPANSVEHQQKTPNSPKEQQKSSVGFTNMQQMASANSLQQQNSSASSLQQQNSSVHSLQKRKSWVNSSQQQRSSVNFIQKQRSWVNSSHHQKSSASSLQQKSSENSPSKFQQQQQKSPANNERSQNNQKSPGIQHSWKQQQQQKPKILQNCPKTSVQQQMNPVNHTQSQSERPTELIYLHSRSPAKRASVTNNSSAPIRIAPRQQTTQSSGPSVVHNEDEDVMPPTPNQSERRQRRFPRRNHNPRMVRSLQAVSREFTESLLEGSSGKTPEKEVVPYQQQEKPRQVKSSPEIHASSHMRKLAMGSVLNSNAKEFHPTGESHLTHKVSRLSQRRCRQRFTRSLHAKKNNPREQLPNPDSAPEPILEYTSEIDDQPCPPRQTVDTPIEAIVPPAMIEVSATGNLPPVFYFDQQSGIIWSNSSPQIPVEIQSNSVSQSVFRLASLPCTGVGTTNMDIFTSSPPPIQHMSSSRLPSSISGPTQLSHTLSSPPVENQHQQHLWESCIHQDEDLMYSRPLLDMDQEETQPEQQDQEWQWESEIEPPSFQSENQVVADEHGEENAYLAKDAGPPLERHMIFRAPSQHGQEYPKPDLNCVPSSIKKLHHLISSQYSDYSFVYALSAQISQDCVPMDCFVYLKMILLASIVSIESDEVRAPISLCIIATDSLMANRLLNKVGQLAPRFLGPHEYGLQPTFNALPTRFNWIVASPLLLAQQGVYYAGDWNRLSKDQGCQLEKCIENGAVPVPQLHIDQPLKAAVWTYWQPNNSTNQTLALAKLCPIFGLPIYMGAQASNSLWNSIIQKHNAEGQIVVNDGLSIPEDDMRMLIHLLHQRKTTLTDGAQHMLQKYYVISRKERPNVFSSKTYIVLKQLAECFAKLALRLEVLESDVCVAIFHCEHFVQGIFGAKENQAPPAVFNFNVISCIDPYMNKFTRWLLQYLNSYEDEELGMHADKRRRTDSWDMP DIP protein Q0KHR4 original sequence version: 81 B9979348ED6504F0 mcmr_drome DNA replication licensing factor REC rec CG31293 Protein mini chromosome maintenance-related Protein recombination-defective protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNPTLSRGGAPSRFVRGRGRGGGAYRPYFYFRRNGRVIPAGGNRQPNQGEPGAPDAPSVPPATRQPRGWSRTAGKRGRNAHLDCSFLRPENYAAPEDGLQVQSIAVDNPRAYSGWRLYFLREKYEEGNELATRIMAVEAYYQRNPHTFDFVMIRDRGFFPLCAAGIKSDAQLKEVWPSLSEDIREHPLRTLGTLSLAMHTVVVNHQLDCNDSTTAMVSTPEQYVLPTPRVRKIYARPDDFVPVESIEGISHSRVDTLFSIRGFVSNVGEPSYSLTWQAFRCSRCQMEIAMRQRGTFQPRPYQCKRSECVARDEFVPLRSSPYTRLSIRQIIRVEESSLNLVHDFETSMPAEMDVELRHDLVDAVRVGQEVVVTGILKLQELGDDTTTGDTSNQMQPYLKAVSIRDASSIKREFSERDLEAIVMINAEPNSFKLLVQSIAPEVYGHELPKAACLLSLLGGKGAETEAINVLLVGDPGIGKTKILQSCAQIAERGAHVSGKRGAQSAQQLGVTFAGRNKRVLQAGSLMMASGGGHCTLDDVDKLASKQAVLLQCLQSEEVNLPLAGAFASFPAQPSVIACANPQRGQYDEGRYLLQNINISPSLLREFHLVYILLDKPSERDMSLTAHVRALHAGARKRARIAARYALKPKMSDSMCEVSLNVPAAGKDDDTIKTEDDNDSIMQQDYDLDKRLEVLPEEGDLDLLPPILIKKFLSYARQELNPVLNEDASNAVLRYFLELKGSCNLDEDVSSQIGAGQLLAIIHLSQARARLDLSHVVSPQHVRDVIALLTESITQTSLKEGSSRQGTRGGGGAGGGAGKSAQLRNFLELTKRRSAALGRRIFEFDELKEIGTRAGILTGFSQLVEMANLGGYLLMKGANMYEVVPD DIP protein Q9VF30 original sequence version: 114 9CDC8D6CF91C21C6 q86b50_drome mei-217 CG33935 Dmel\CG33935 Dmel_CG33935 CG33067 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MFTVDASEGSSVVNETPLKSQFHDQKLVEHFCRQTQLLKLVQVSSGRFVANYMSSRRPEDLLAPIQLRLDFEYRQLLDISPQLLQLIVEEPLQFQEAVRYSVYGLIRSHLKDAGLKPIDINQLHAHWRLVGLPFTPGLQFEPRDQLTRLGLSQIRGILAAFTPQETLVLQSIWYCGSGCMRNAIQTSSTDAPFCSSCSRPMSEYQKLRVTETYRILAVLPISAVQTPRVTNCLHRPKLVRLRAHAHDCELKLGASYLITGYFASSASTYQLEACHLRIN DIP protein Q86B50 original sequence version: 97 832452E5DBFBDC5B rec-mei_217-1 3 6 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association mei_217-mei_218-1 1 4 unspecified role unspecified role prey prey Region 396-791 Region 396-791 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 792-1186 Region 792-1186 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 6 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association mei_217-rec-1 2 6 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association