DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation. Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation. Nature chemical biology 2013 imex curation Wang AM., Miyata Y., Klinedinst S., Peng HM., Chua JP., Komiyama T., Li X., Morishima Y., Merry DE., Pratt WB., Osawa Y., Collins CA., Gestwicki JE., Lieberman AP. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang AM., Miyata Y., Klinedinst S., Peng HM., Chua JP., Komiyama T., Li X., Morishima Y., Merry DE., Pratt WB., Osawa Y., Collins CA., Gestwicki JE., Lieberman AP. DIP original experiment: DIP-266858X Nature chemical biology 2013 IMEx Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation. Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation. Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation. Nature chemical biology 2013 imex curation Wang AM., Miyata Y., Klinedinst S., Peng HM., Chua JP., Komiyama T., Li X., Morishima Y., Merry DE., Pratt WB., Osawa Y., Collins CA., Gestwicki JE., Lieberman AP. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Wang AM., Miyata Y., Klinedinst S., Peng HM., Chua JP., Komiyama T., Li X., Morishima Y., Merry DE., Pratt WB., Osawa Y., Collins CA., Gestwicki JE., Lieberman AP. DIP original experiment: DIP-266859X Nature chemical biology 2013 IMEx Activation of Hsp70 reduces neurotoxicity by promoting polyglutamine protein degradation. nos1_human Nitric oxide synthase, brain NOS1 Peptidyl-cysteine S-nitrosylase NOS1 bNOS NOS type I Neuronal NOS Constitutive NOS NC-NOS protein protein human Homo sapiens Human MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIISDLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEGFTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQHAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTSGSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLNNPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEYICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKEIDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNHVKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTEICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKWYGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMRKTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWVWIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLAEAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDIVHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSYSDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGERILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRNKFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDELRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWGKNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRTRDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSAEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS DIP protein P29475 original sequence version: 159 DIP protein P29475 original sequence version: 177 mint Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter Genetic variations in NOS1 gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1) [MIM:179010]. IHPS has an incidence of 1-5 per 1'000 live births in whites and a marked preponderance of males to females (4:1). IHPS is the most frequent disorder requiring surgery in the first year of life. The disorder is characterized by hypertrophy and hyperplasia of the circular muscle layer of the pylorus, leading to persistent vomiting 2-12 weeks after birth. Defective pyloric relaxation and increased pyloric smooth muscle mass have been suggested to be responsible for gastric-outlet obstruction 99235793B953BF37 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG nos1-ub-1 1 3 unspecified role unspecified role bait bait human Homo sapiens Human 4 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association nos1-ub-2 2 3 unspecified role unspecified role bait bait human Homo sapiens Human 4 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal human Homo sapiens Human physical association physical association