IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1. Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1. Journal of immunology (Baltimore, Md. : 1950) 2012 imex curation Fujita M., Takada YK., Takada Y. same group in 2014 paper - annotated + released pmid:24789099. It is very unclear what the origins of Integrin constructs are. Recombinant soluble alpha-v/Beta-3;according to Ref 33 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. ytakada@ucdavis.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Fujita M., Takada YK., Takada Y. same group in 2014 paper - annotated + released pmid:24789099. It is very unclear what the origins of Integrin constructs are. Recombinant soluble alpha-v/Beta-3;according to Ref 33 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. ytakada@ucdavis.edu Accepted 2017-OCT-18 AT 09:43 BST AT 09:43 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2012 imex curation Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1. Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1. Journal of immunology (Baltimore, Md. : 1950) 2012 imex curation Fujita M., Takada YK., Takada Y. same group in 2014 paper - annotated + released pmid:24789099. It is very unclear what the origins of Integrin constructs are. Recombinant soluble alpha-v/Beta-3;according to Ref 33 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. ytakada@ucdavis.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Fujita M., Takada YK., Takada Y. same group in 2014 paper - annotated + released pmid:24789099. It is very unclear what the origins of Integrin constructs are. Recombinant soluble alpha-v/Beta-3;according to Ref 33 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. ytakada@ucdavis.edu Accepted 2017-OCT-18 AT 09:43 BST AT 09:43 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2012 imex curation Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1. Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1. Journal of immunology (Baltimore, Md. : 1950) 2012 imex curation Fujita M., Takada YK., Takada Y. same group in 2014 paper - annotated + released pmid:24789099. It is very unclear what the origins of Integrin constructs are. Recombinant soluble alpha-v/Beta-3;according to Ref 33 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. ytakada@ucdavis.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Fujita M., Takada YK., Takada Y. same group in 2014 paper - annotated + released pmid:24789099. It is very unclear what the origins of Integrin constructs are. Recombinant soluble alpha-v/Beta-3;according to Ref 33 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. ytakada@ucdavis.edu Accepted 2017-OCT-18 AT 09:43 BST AT 09:43 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2012 imex curation Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1. Integrins αvβ3 and α4β1 act as coreceptors for fractalkine, and the integrin-binding defective mutant of fractalkine is an antagonist of CX3CR1. Journal of immunology (Baltimore, Md. : 1950) 2012 imex curation Fujita M., Takada YK., Takada Y. same group in 2014 paper - annotated + released pmid:24789099. It is very unclear what the origins of Integrin constructs are. Recombinant soluble alpha-v/Beta-3;according to Ref 33 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. ytakada@ucdavis.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fujita M., Takada YK., Takada Y. same group in 2014 paper - annotated + released pmid:24789099. It is very unclear what the origins of Integrin constructs are. Recombinant soluble alpha-v/Beta-3;according to Ref 33 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. ytakada@ucdavis.edu Accepted 2017-OCT-18 AT 09:43 BST AT 09:43 BST by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2012 imex curation itb3_human Integrin beta-3 Platelet membrane glycoprotein IIIa ITGB3 GP3A protein protein human Homo sapiens Human MRARPRPRPLWATVLALGALAGVGVGGPNICTTRGVSSCQQCLAVSPMCAWCSDEALPLGSPRCDLKENLLKDNCAPESIEFPVSEARVLEDRPLSDKGSGDSSQVTQVSPQRIALRLRPDDSKNFSIQVRQVEDYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPEALENPCYDMKTTCLPMFGYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVQPNDGQCHVGSDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVNLYQNYSELIPGTTVGVLSMDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCMGLKIGDTVSFSIEAKVRGCPQEKEKSFTIKPVGFKDSLIVQVTFDCDCACQAQAEPNSHRCNNGNGTFECGVCRCGPGWLGSQCECSEEDYRPSQQDECSPREGQPVCSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCSCGDCLCDSDWTGYYCNCTTRTDTCMSSNGLLCSGRGKCECGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFDRGALHDENTCNRYCRDEIESVKELKDTGKDAVNCTYKNEDDCVVRFQYYEDSSGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLAALLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT DIP protein P05106 original sequence version: 202 DIP protein P05106-1 original sequence version: 158 DIP protein P05106-1 original sequence version: 164 DIP protein P05106 original sequence version: 200 DIP protein P05106-1 original sequence version: 213 DIP protein P05106-1 original sequence version: 175 DIP protein P05106-1 original sequence version: 192 DIP protein P05106-1 original sequence version: 171 F246623608E05F9E cx3c1_human CX3C chemokine receptor 1 CX3CR1 Beta chemokine receptor-like 1 CMKBRL1 Fractalkine receptor G-protein coupled receptor 13 GPR13 V28 protein protein human Homo sapiens Human MDQFPESVTENFEYDDLAEACYIGDIVVFGTVFLSIFYSVIFAIGLVGNLLVVFALTNSKKPKSVTDIYLLNLALSDLLFVATLPFWTHYLINEKGLHNAMCKFTTAFFFIGFFGSIFFITVISIDRYLAIVLAANSMNNRTVQHGVTISLGVWAAAILVAAPQFMFTKQKENECLGDYPEVLQEIWPVLRNVETNFLGFLLPLLIMSYCYFRIIQTLFSCKNHKKAKAIKLILLVVIVFFLFWTPYNVMIFLETLKLYDFFPSCDMRKDLRLALSVTETVAFSHCCLNPLIYAFAGEKFRRYLYHLYGKCLAVLCGRSVHVDFSSSESQRSRHGSVLSSNFTYHTSDGDALLLL C59DC5F4C4312F22 ita5_human Integrin alpha-5 ITGA5 FNRA Fibronectin receptor subunit alpha Integrin alpha-F VLA-5 CD49 antigen-like family member E protein protein human Homo sapiens Human MGSRTPESPLHAVQLRWGPRRRPPLLPLLLLLLPPPPRVGGFNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSYGVPLWIIILAILFGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA DIP protein P08648 original sequence version: 123 DIP protein P08648 original sequence version: 137 6B4D558D4F739CBA ita4_human Integrin alpha-4 Integrin alpha-IV VLA-4 subunit alpha CD49 antigen-like family member D ITGA4 CD49D protein protein human Homo sapiens Human MAWEARREPGPRRAAVRETVMLLLCLGVPTGRPYNVDTESALLYQGPHNTLFGYSVVLHSHGANRWLLVGAPTANWLANASVINPGAIYRCRIGKNPGQTCEQLQLGSPNGEPCGKTCLEERDNQWLGVTLSRQPGENGSIVTCGHRWKNIFYIKNENKLPTGGCYGVPPDLRTELSKRIAPCYQDYVKKFGENFASCQAGISSFYTKDLIVMGAPGSSYWTGSLFVYNITTNKYKAFLDKQNQVKFGSYLGYSVGAGHFRSQHTTEVVGGAPQHEQIGKAYIFSIDEKELNILHEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSTIREEGRVFVYINSGSGAVMNAMETNLVGSDKYAARFGESIVNLGDIDNDGFEDVAIGAPQEDDLQGAIYIYNGRADGISSTFSQRIEGLQISKSLSMFGQSISGQIDADNNGYVDVAVGAFRSDSAVLLRTRPVVIVDASLSHPESVNRTKFDCVENGWPSVCIDLTLCFSYKGKEVPGYIVLFYNMSLDVNRKAESPPRFYFSSNGTSDVITGSIQVSSREANCRTHQAFMRKDVRDILTPIQIEAAYHLGPHVISKRSTEEFPPLQPILQQKKEKDIMKKTINFARFCAHENCSADLQVSAKIGFLKPHENKTYLAVGSMKTLMLNVSLFNAGDDAYETTLHVKLPVGLYFIKILELEEKQINCEVTDNSGVVQLDCSIGYIYVDHLSRIDISFLLDVSSLSRAEEDLSITVHATCENEEEMDNLKHSRVTVAIPLKYEVKLTVHGFVNPTSFVYGSNDENEPETCMVEKMNLTFHVINTGNSMAPNVSVEIMVPNSFSPQTDKLFNILDVQTTTGECHFENYQRVCALEQQKSAMQTLKGIVRFLSKTDKRLLYCIKADPHCLNFLCNFGKMESGKEASVHIQLEGRPSILEMDETSALKFEIRATGFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRYFTIVIISSSLLLGLIVLLLISYVMWKAGFFKRQYKSILQEENRRDSWSYINSKSNDD DIP protein P13612 original sequence version: 131 DIP protein P13612-1 original sequence version: 171 DIP protein P13612 original sequence version: 138 73EC1204DE78CD35 a0a061ij01_crigr H671_2g5979 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MGSRTPRSPQTPLHAVPLRWGPSRLPPLLPLLLLLWPPPLQVGGFNLDAEAPAVLSGPPGSLFGFSVEFYRPGRDGVSVLVGAPKANTSQPGVLQGGAVYVCPWDTSPAQCTTIQFDSKGSRILESSLSSAKGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPQSDPVGTCYLSTDNFTRILEYAPCRSDFSTAAGQGYCQGGFSAEFTKTGHVVLGGPGSYFWQGQILSATQEQISESYYPEYLINPVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIHSLYNVSGEQMASYFGYAVAATDTNGDGLDDLLVGAPLLMERTADGRPQEVGRVYIYLQHPAGIEPTPTLTLTGQDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFIFPGGPGGLNTKPSQILQPLWAAGSTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKALVYRGRPIISASASLTIFPSMFNPEERSCSLEGNPVSCINLSFCLNASGKHVPNSIGFEVELQLDWQKQKGGIRRALFLASKQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPKAPMDSHGLRPVLHYQSKSRIEDKAQILLDCGEDNICVPDLQLDVYGEKKHVYLGDKNSLNLTFHAQNLGEGGAYEAELRVTAPLEAEYSGLVRHPGNFSSLSCDYFAVNQSRQLVCDLGNPMKAGTSIWGGLRFTVPHLQDTKKTIQFDFQILSKNLNNSQSNVVSFPLSVEAQAQVSLNGVSKPEAVLFPVSDWYPQDQPQKEGDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLSNCTSNYTPNSQGLELDPESFPHHLQKREAPGRSSTASGPQVLKCPEAKCFMLRCEFGPLHRQESRSLQLHFRVWAKTFLQREHQPFSLQCEAVYEALKMPYQILPRQLPQKKLQVATAVQWTKAEGSNGVPLWIIILAILFGLLLLGLLIYVLYKLGFFKRSLPYGTAMEKAQLKPPATSDA 445A37B1FEC8266C x3cl1_human Fractalkine C-X3-C motif chemokine 1 CX3C membrane-anchored chemokine Neurotactin Small-inducible cytokine D1 A-152E5.2 CX3CL1 FKN NTT SCYD1 protein protein human Homo sapiens Human MAPISLSWLLRLATFCHLTVLLAGQHHGVTKCNITCSKMTSKIPVALLIHYQQNQASCGKRAIILETRQHRLFCADPKEQWVKDAMQHLDRQAAALTRNGGTFEKQIGEVKPRTTPAAGGMDESVVLEPEATGESSSLEPTPSSQEAQRALGTSPELPTGVTGSSGTRLPPTPKAQDGGPVGTELFRVPPVSTAATWQSSAPHQPGPSLWAEAKTSEAPSTQDPSTQASTASSPAPEENAPSEGQRVWGQGQSPRPENSLEREEMGPVPAHTDAFQDWGPGSMAHVSVVPVSSEGTPSREPVASGSWTPKAEEPIHATMDPQRLGVLITPVPDAQAATRRQAVGLLAFLGLLFCLGVAMFTYQSLQGCPRKMAGEMAEGLRYIPRSCGSNSYVLVPV DIP protein P78423 original sequence version: 143 BAC71F5080DF9CA6 a0a061ij01_crigr-cx3cl1-3 1 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 10 unspecified role unspecified role bait bait P78423:p.Arg71Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:50:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg71ala' Tue Jun 30 18:05:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg71Ala' P78423:p.Lys60_Arg61delinsGluGlu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain KR EE Wed May 02 14:52:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_arg60-61glu_glu' Tue Jun 30 18:23:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys60_Arg61delinsGluGlu' P78423:p.Lys60_Arg61delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain KR AA Wed May 02 16:53:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_arg60-61ala_ala' Tue Jun 30 19:02:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys60_Arg61delinsAlaAla' P78423:p.[Lys78Ala;Lys83Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:40:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys78ala,lys83ala' Tue Jun 30 18:52:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys78Ala;Lys83Ala]' P78423:p.Lys78Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:53:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys78ala' Tue Jun 30 16:01:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys78Ala' chemokine_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 13 23 physical association physical association IB, 3A/B Heat denaturation cx3cl1-a0a061ij01_crigr-1 3 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 10 unspecified role unspecified role prey prey chemokine_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 26 31 physical association physical association 1E KD of 1.1 × 10−8 M in 1 mM Ca2+ (Fig. 1E), KD of 6.9 × 10−9 M in 1 mM Mg2+, and KD of 3.0 × 10−10 M in 1 mM Mn2+(data not shown) cx3cl1-a0a061ij01_crigr-2 1 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells 10 unspecified role unspecified role bait bait P78423:p.Arg71Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:31:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg71ala' Tue Jun 30 17:28:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg71Ala' P78423:p.Lys60_Arg61delinsGluGlu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain KR EE Wed May 02 19:39:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_arg60-61glu_glu' Tue Jun 30 20:23:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys60_Arg61delinsGluGlu' P78423:p.Lys60_Arg61delinsAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain KR AA Wed May 02 16:12:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_arg60-61ala_ala' Tue Jun 30 16:55:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys60_Arg61delinsAlaAla' P78423:p.[Lys78Ala;Lys83Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 20:16:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys78ala,lys83ala' Tue Jun 30 15:36:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys78Ala;Lys83Ala]' P78423:p.Lys78Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:31:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys78ala' Tue Jun 30 19:47:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys78Ala' chemokine_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells physical association physical association I C/D, 3C/D Mn2+ ions cx3cl1-cx3cr1-1 2 10 unspecified role unspecified role bait bait P78423:p.Arg71Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:57:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg71ala' Tue Jun 30 15:35:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg71Ala' P78423:p.Lys60_Arg61delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain KR AA Wed May 02 15:04:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_arg60-61ala_ala' Tue Jun 30 15:50:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys60_Arg61delinsAlaAla' chemokine_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells physical association physical association 3E 467y4rturtju-1 4 10 ancillary ancillary ancillary ancillary n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemokine_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P78423:p.Lys60_Arg61delinsGluGlu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KR EE Wed May 02 14:40:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_arg60-61glu_glu' Tue Jun 30 19:52:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys60_Arg61delinsGluGlu' P78423:p.Arg71Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:34:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg71ala' Tue Jun 30 17:39:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg71Ala' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role bait bait human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells 6 unspecified role unspecified role prey prey human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells association association 5F itga4-cx3cr1-1 4 10 ancillary ancillary ancillary ancillary n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemokine_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P78423:p.Lys60_Arg61delinsGluGlu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain KR EE Wed May 02 16:34:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys_arg60-61glu_glu' Tue Jun 30 15:41:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys60_Arg61delinsGluGlu' P78423:p.Arg71Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:14:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg71ala' Tue Jun 30 16:36:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg71Ala' ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells 6 unspecified role unspecified role prey prey human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells association association 5G itgb3-cx3cr1-1 4 10 ancillary ancillary ancillary ancillary region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 5 unspecified role unspecified role bait bait human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells 6 unspecified role unspecified role prey prey human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells association association Supp fig 2 itgb3-cx3cr1-2 4 10 ancillary ancillary ancillary ancillary cell lysate cell lysate region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 8 unspecified role unspecified role bait bait human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells 6 unspecified role unspecified role prey prey human-u937 Homo sapiens histiocytic lymphoma cells u937 Human histiocytic lymphoma cells association association Supp fig 2i