DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Nature 2012 imex curation Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251219X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Nature 2012 imex curation Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251218X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Nature 2012 imex curation Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251215X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Nature 2012 imex curation Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251217X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Nature 2012 imex curation Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251214X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. Nature 2012 imex curation Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Straub AC., Lohman AW., Billaud M., Johnstone SR., Dwyer ST., Lee MY., Bortz PS., Best AK., Columbus L., Gaston B., Isakson BE. DIP original experiment: DIP-251216X Nature 2012 IMEx Endothelial cell expression of haemoglobin α regulates nitric oxide signalling. nos3_human Nitric oxide synthase, endothelial Endothelial NOS EC-NOS NOS type III Constitutive NOS NOS3 protein protein human Homo sapiens Human MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP DIP protein P29474 original sequence version: 179 DIP protein P29474 original sequence version: 175 DIP protein P29474 original sequence version: 112 DIP protein P29474 original sequence version: 140 DIP protein P29474 original sequence version: 158 B761A6D40B1A5649 nos3_mouse Nitric oxide synthase, endothelial Nos3 Ecnos Endothelial NOS EC-NOS NOS type III Constitutive NOS protein protein mouse Mus musculus Mouse MGNLKSVGQEPGPPCGLGLGLGLGLCGKQGPASPAPEPSQAPAPPSPTRPAPDHSPPLTRPPDGPRFPRVKNWEVGSITYDTLSAQAQQDGPCTSRRCLGSLVFPRKLQSRPTQGPSPTEQLLGQARDFINQYYNSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRTAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLIRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDEPPELFTLPPEMVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSSEIGMRDLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGAGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHEALVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSVSCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSTQAESLQLLPGLTHVHRRKMFQATILSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPPSTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTYFLDITSPPSPRLLRLLSTLAEESSEQQELEALSQDPRRYEEWKWFSCPTLLEVLEQFPSVALPAPLILTQLPLLQPRYYSVSSAPSAHPGEIHLTIAVLAYRTQDGLGPLHYGVCSTWMSQLKAGDPVPCFIRGAPSFRLPPDPNLPCILVGPGTGIAPFRGFWQDRLHDIEIKGLQPAPMTLVFGCRCSQLDHLYRDEVLDAQQRGVFGQVLTAFSRDPGSPKTYVQDLLRTELAAEVHRVLCLEQGHMFVCGDVTMATSVLQTVQRILATEGGMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWSFDPPGPEIPGS DIP protein P70313 original sequence version: 131 E1F65C43601F0937 nb5r3_mouse NADH-cytochrome b5 reductase 3 Cyb5r3 Dia1 Diaphorase-1 protein protein mouse Mus musculus Mouse MGAQLSTLSHVVLSPVWFIYSLFMKLFQRSTPAITLENPDIKYPLRLIDKEVISPDTRRFRFALPSPQHILGLPIGQHIYLSTRIDGNLVIRPYTPVSSDDDKGFVDLVVKVYFKDTHPKFPAGGKMSQYLENMKIGDTIEFRGPNGLLVYQGKGKFAIRADKKSNPVVRTVKSVGMIAGGTGITPMLQVIRAVLKDPNDHTVCYLLFANQSEKDILLRPELEELRNEHSARFKLWYTVDKAPDAWDYSQGFVNEEMIRDHLPTPGEEPLILMCGPPPMIQFACLPNLERVGHPKERCFTF DIP protein Q9DCN2 original sequence version: 98 984D5B73430F725D nb5r3_human NADH-cytochrome b5 reductase 3 Diaphorase-1 CYB5R3 DIA1 protein protein human Homo sapiens Human MGAQLSTLGHMVLFPVWFLYSLLMKLFQRSTPAITLESPDIKYPLRLIDREIISHDTRRFRFALPSPQHILGLPVGQHIYLSARIDGNLVVRPYTPISSDDDKGFVDLVIKVYFKDTHPKFPAGGKMSQYLESMQIGDTIEFRGPSGLLVYQGKGKFAIRPDKKSNPIIRTVKSVGMIAGGTGITPMLQVIRAIMKDPDDHTVCHLLFANQTEKDILLRPELEELRNKHSARFKLWYTLDRAPEAWDYGQGFVNEEMIRDHLPPPEEEPLVLMCGPPPMIQYACLPNLDHVGHPTERCFVF DIP protein P00387 original sequence version: 176 FDCDCDC4EC3570B4 hba_human Hemoglobin subunit alpha Hemoglobin alpha chain Alpha-globin HBA1 HBA2 protein protein human Homo sapiens Human MVLSPADKTNVKAAWGKVGAHAGEYGAEALERMFLSFPTTKTYFPHFDLSHGSAQVKGHGKKVADALTNAVAHVDDMPNALSALSDLHAHKLRVDPVNFKLLSHCLLVTLAAHLPAEFTPAVHASLDKFLASVSTVLTSKYR DIP protein P69905 original sequence version: 112 DIP protein P69905 original sequence version: 132 15E13666573BBBAE hba_mouse Hemoglobin subunit alpha Hemoglobin alpha chain Alpha-globin Hba Hba-a1 protein protein mouse Mus musculus Mouse MVLSGEDKSNIKAAWGKIGGHGAEYGAEALERMFASFPTTKTYFPHFDVSHGSAQVKGHGKKVADALASAAGHLDDLPGALSALSDLHAHKLRVDPVNFKLLSHCLLVTLASHHPADFTPAVHASLDKFLASVSTVLTSKYR DIP protein P01942 original sequence version: 116 2F70043BFF66E24A hba-nos3-1 5 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse 12 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association hba1-cyb5r3-1 1 10 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association hba-cyb5r3-1 4 9 unspecified role unspecified role prey prey mouse Mus musculus Mouse 12 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association hba1-nos3-1 3 7 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association cyb5r3-hba1-1 2 10 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 11 unspecified role unspecified role prey prey human Homo sapiens Human direct interaction direct interaction nos3-hba1-1 6 7 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 11 unspecified role unspecified role prey prey human Homo sapiens Human direct interaction direct interaction