DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structure (London, England : 1993) 2012 imex curation Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251331X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structure (London, England : 1993) 2012 imex curation Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251329X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structure (London, England : 1993) 2012 imex curation Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251333X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structure (London, England : 1993) 2012 imex curation Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251330X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structure (London, England : 1993) 2012 imex curation Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251328X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. Structure (London, England : 1993) 2012 imex curation Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. Only protein-protein interactions ecolx Escherichia coli saxs x ray scattering saxs predetermined predetermined participant predetermined Pellegrino S., Radzimanowski J., de Sanctis D., Boeri Erba E., McSweeney S., Timmins J. DIP original experiment: DIP-251332X Structure (London, England : 1993) 2012 IMEx Structural and functional characterization of an SMC-like protein RecN: new insights into double-strand break repair. recn_deira DNA repair protein RecN recN Recombination protein N DR_1477 protein protein strain atcc 13939 /-9 Deinococcus radiodurans MTRKARTPKAAPVPEAVAVVEPPPPDAAPTGPRLSRLEIRNLATITQLELELGGGFCAFTGETGAGKSIIVDALGLLLGGRANHDLIRSGEKELLVTGFWGDGDESEADSASRRLSSAGRGAARLSGEVVSVRELQEWAQGRLTIHWQHSAVSLLSPANQRGLLDRRVTKEAQAYAAAHAAWREAVSRLERLQASQRERARQIDLLAFQVQEISEVSPDPGEEEGLNTELSRLSNLHTIAQAAAGGVELLSDGDLNAAGLIGEAVRALNAGAKYDETVMQLQNELRAALESVQAIAGELRDVAEGSAADPEALDRVEARLSALSKLKNKYGPTLEDVVEFGAQAAEELAGLEEDERDAGSLQADVDALHAELLKVGQALDAAREREAEPLVDSLLAVIRELGMPHARMEFALSALAEPAAYGLSDVLLRFSANPGEELGPLSDVASGGELSRVMLAVSTVLGADTPSVVFDEVDAGIGGAAAIAVAEQLSRLADTRQVLVVTHLAQIAARAHHHYKVEKQVEDGRTVSHVRLLTGDERLEEIARMLSGNTSEAALEHARELLAG DIP protein Q9WXF2 original sequence version: 77 recn-1 5 7 unspecified role unspecified role unspecified role unspecified role Coiled-coil domain Region 196-365 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 196-365 Region 196-365 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 237-291 Region 237-291 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction recn-2 1 7 unspecified role unspecified role unspecified role unspecified role Region 196-365 Region 196-365 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction recn-3 2 7 unspecified role unspecified role unspecified role unspecified role Coiled-coil domain Region 196-365 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 196-365 Region 196-365 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 237-291 Region 237-291 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction recn-4 6 7 unspecified role unspecified role unspecified role unspecified role Region 1-195/366-564 Region 1-195/366-564 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Q9WXF2:p.[Lys67Ala;Glu472Gln] K67A/E472Q mutation mutation certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain E Q This feature has been corrected as a result of our quality control procedures. The original label was 'K67A/E472Q'. Wed May 02 18:08:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys67ala,glu472gln' Tue Jun 30 16:16:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys67Ala;Glu472Gln]' ecolx Escherichia coli direct interaction direct interaction recn-5 4 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction recn-6 3 7 unspecified role unspecified role unspecified role unspecified role Region 1-195/366-564 Region 1-195/366-564 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Q9WXF2:p.[Lys67Ala;Glu472Gln] K67A/E472Q mutation mutation certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain E Q This feature has been corrected as a result of our quality control procedures. The original label was 'K67A/E472Q'. Wed May 02 16:27:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys67ala,glu472gln' Tue Jun 30 19:05:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys67Ala;Glu472Gln]' Q9WXF2:p.[Lys67Ala;Glu472Ala] K67A/E472A mutation mutation certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'K67A/E472A'. Wed May 02 18:42:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys67ala,glu472ala' Tue Jun 30 18:34:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys67Ala;Glu472Ala]' Q9WXF2:p.Lys67Ala K67A mutation mutation certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K67A'. Wed May 02 15:46:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys67ala' Tue Jun 30 16:11:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys67Ala' Q9WXF2:p.Glu472Gln E472Q mutation mutation certain certain sequence position certain certain certain sequence position certain E Q This feature has been corrected as a result of our quality control procedures. The original label was 'E472Q'. Wed May 02 18:56:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu472gln' Tue Jun 30 19:36:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu472Gln' Q9WXF2:p.Glu472Ala E472A mutation mutation certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E472A'. Wed May 02 14:43:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu472ala' Tue Jun 30 18:13:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu472Ala' ecolx Escherichia coli direct interaction direct interaction