DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. Structure (London, England : 1993) 2012 imex curation Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. DIP original experiment: DIP-251199X Structure (London, England : 1993) 2012 IMEx RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. Structure (London, England : 1993) 2012 imex curation Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. DIP original experiment: DIP-251193X Structure (London, England : 1993) 2012 IMEx RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. Structure (London, England : 1993) 2012 imex curation Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. DIP original experiment: DIP-251194X Structure (London, England : 1993) 2012 IMEx RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. Structure (London, England : 1993) 2012 imex curation Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. Only protein-protein interactions in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. DIP original experiment: DIP-251197X Structure (London, England : 1993) 2012 IMEx RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. Structure (London, England : 1993) 2012 imex curation Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. DIP original experiment: DIP-251196X Structure (London, England : 1993) 2012 IMEx RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. Structure (London, England : 1993) 2012 imex curation Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. DIP original experiment: DIP-251195X Structure (London, England : 1993) 2012 IMEx RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. Structure (London, England : 1993) 2012 imex curation Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. DIP original experiment: DIP-251198X Structure (London, England : 1993) 2012 IMEx RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. Structure (London, England : 1993) 2012 imex curation Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nelson CA., Warren JT., Wang MW., Teitelbaum SL., Fremont DH. DIP original experiment: DIP-251192X Structure (London, England : 1993) 2012 IMEx RANKL employs distinct binding modes to engage RANK and the osteoprotegerin decoy receptor. tr11b_mouse Tumor necrosis factor receptor superfamily member 11B Tnfrsf11b Ocif Opg Osteoclastogenesis inhibitory factor Osteoprotegerin protein protein mouse Mus musculus Mouse MNKWLCCALLVLLDIIEWTTQETLPPKYLHYDPETGHQLLCDKCAPGTYLKQHCTVRRKTLCVPCPDHSYTDSWHTSDECVYCSPVCKELQSVKQECNRTHNRVCECEEGRYLEIEFCLKHRSCPPGSGVVQAGTPERNTVCKKCPDGFFSGETSSKAPCIKHTNCSTFGLLLIQKGNATHDNVCSGNREATQKCGIDVTLCEEAFFRFAVPTKIIPNWLSVLVDSLPGTKVNAESVERIKRRHSSQEQTFQLLKLWKHQNRDQEMVKKIIQDIDLCESSVQRHLGHSNLTTEQLLALMESLPGKKISPEEIERTRKTCKSSEQLLKLLSLWRIKNGDQDTLKGLMYALKHLKTSHFPKTVTHSLRKTMRFLHSFTMYRLYQKLFLEMIGNQVQSVKISCL DIP protein O08712 original sequence version: 100 tnr11_mouse Tumor necrosis factor receptor superfamily member 11A Receptor activator of NF-KB Osteoclast differentiation factor receptor Tnfrsf11a Rank protein protein mouse Mus musculus Mouse MAPRARRRRQLPAPLLALCVLLVPLQVTLQVTPPCTQERHYEHLGRCCSRCEPGKYLSSKCTPTSDSVCLPCGPDEYLDTWNEEDKCLLHKVCDAGKALVAVDPGNHTAPRRCACTAGYHWNSDCECCRRNTECAPGFGAQHPLQLNKDTVCTPCLLGFFSDVFSSTDKCKPWTNCTLLGKLEAHQGTTESDVVCSSSMTLRRPPKEAQAYLPSLIVLLLFISVVVVAAIIFGVYYRKGGKALTANLWNWVNDACSSLSGNKESSGDRCAGSHSATSSQQEVCEGILLMTREEKMVPEDGAGVCGPVCAAGGPWAEVRDSRTFTLVSEVETQGDLSRKIPTEDEYTDRPSQPSTGSLLLIQQGSKSIPPFQEPLEVGENDSLSQCFTGTESTVDSEGCDFTEPPSRTDSMPVSPEKHLTKEIEGDSCLPWVVSSNSTDGYTGSGNTPGEDHEPFPGSLKCGPLPQCAYSMGFPSEAAASMAEAGVRPQDRADERGASGSGSSPSDQPPASGNVTGNSNSTFISSGQVMNFKGDIIVVYVSQTSQEGPGSAEPESEPVGRPVQEETLAHRDSFAGTAPRFPDVCATGAGLQEQGAPRQKDGTSRPVQEQGGAQTSLHTQGSGQCAE DIP protein O35305 original sequence version: 82 DIP protein O35305 original sequence version: 91 DIP protein O35305 original sequence version: 102 F8C1872E99511D8E o35235-1 Tumor necrosis factor ligand superfamily member 11 Tnfsf11 Opgl Rankl Trance Osteoclast differentiation factor Osteoprotegerin ligand Receptor activator of nuclear factor kappa-B ligand TNF-related activation-induced cytokine protein protein mouse Mus musculus Mouse MRRASRDYGKYLRSSEEMGSGPGVPHEGPLHPAPSAPAPAPPPAASRSMFLALLGLGLGQVVCSIALFLYFRAQMDPNRISEDSTHCFYRILRLHENADLQDSTLESEDTLPDSCRRMKQAFQGAVQKELQHIVGPQRFSGAPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID DIP protein O35235-1 original sequence version: 115 DIP protein O35235-1 original sequence version: 127 tnfsf11-1 8 11 unspecified role unspecified role unspecified role unspecified role Region 162-316 Region 162-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tnfsf11-tnfrsf11a-1 7 10 unspecified role unspecified role prey prey Region 1-198 Region 1-198 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 11 unspecified role unspecified role bait bait Region 162-316 Region 162-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tnfrsf11b-tnfsf11-3 2 9 unspecified role unspecified role unspecified role unspecified role Region 22-197 Region 22-197 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 11 unspecified role unspecified role unspecified role unspecified role Region 162-316 Region 162-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tnfrsf11a-tnfsf11-14 6 10 unspecified role unspecified role unspecified role unspecified role Region 1-198 Region 1-198 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 11 unspecified role unspecified role unspecified role unspecified role Region 162-316 Region 162-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tnfsf11-tnfrsf11b-3 4 9 unspecified role unspecified role prey prey Region 22-191 Region 22-191 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O08712:p.Phe117Ala F117A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F117A'. Wed May 02 16:01:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe117ala' Tue Jun 30 19:20:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe117Ala' human Homo sapiens Human 11 unspecified role unspecified role bait bait Region 162-316 Region 162-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tnfsf11-tnfrsf11a-2 1 10 unspecified role unspecified role prey prey Region 1-198 Region 1-198 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 11 unspecified role unspecified role bait bait Region 162-316 Region 162-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tnfrsf11b-tnfsf11-4 3 9 unspecified role unspecified role unspecified role unspecified role Region 22-197 Region 22-197 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 11 unspecified role unspecified role unspecified role unspecified role Region 162-316 Region 162-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tnfsf11-tnfrsf11b-4 5 9 unspecified role unspecified role prey prey Region 22-191 Region 22-191 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O08712:p.Phe117Ala F117A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F117A'. Wed May 02 15:06:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe117ala' Tue Jun 30 15:54:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe117Ala' O08712:p.Phe117Leu F117L mutation increasing strength mutation increasing interaction strength mutation increasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F L This feature has been corrected as a result of our quality control procedures. The original label was 'F117L'. Wed May 02 20:10:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe117leu' Tue Jun 30 18:16:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe117Leu' human Homo sapiens Human 11 unspecified role unspecified role bait bait Region 162-316 Region 162-316 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction