DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural evolution of the membrane-coating module of the nuclear pore complex. Structural evolution of the membrane-coating module of the nuclear pore complex. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Liu X., Mitchell JM., Wozniak RW., Blobel G., Fan J. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Liu X., Mitchell JM., Wozniak RW., Blobel G., Fan J. DIP original experiment: DIP-251799X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural evolution of the membrane-coating module of the nuclear pore complex. Structural evolution of the membrane-coating module of the nuclear pore complex. Structural evolution of the membrane-coating module of the nuclear pore complex. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Liu X., Mitchell JM., Wozniak RW., Blobel G., Fan J. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Liu X., Mitchell JM., Wozniak RW., Blobel G., Fan J. DIP original experiment: DIP-251800X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural evolution of the membrane-coating module of the nuclear pore complex. Structural evolution of the membrane-coating module of the nuclear pore complex. Structural evolution of the membrane-coating module of the nuclear pore complex. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Liu X., Mitchell JM., Wozniak RW., Blobel G., Fan J. Only protein-protein interactions in vitro In vitro ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Liu X., Mitchell JM., Wozniak RW., Blobel G., Fan J. DIP original experiment: DIP-251798X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural evolution of the membrane-coating module of the nuclear pore complex. yeki_schpo Uncharacterized WD repeat-containing protein C4F10.18 SPAC4F10.18 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTLSSNQYQLPLNVRPYTTTWCSQSPSCSNLLAIGHDTGITIYCASEEQTPGSTGLTLQELFTIQTGLPTLHLSFSSSCSYSENLHDGDGNVNSSPVYSLFLACVCQDNTVRLIITKNETIITQHVLGGKSGHHNFVNDIDIADVYSADNRLAEQVIASVGDDCTLIIWRLTDEGPILAGYPLSSPGISVQFRPSNPNQLIVGERNGNIRIFDWTLNLSAEENSQTELVKNPWLLTLNTLPLVNTCHSSGIASSLANVRWIGSDGSGILAMCKSGAWLRWNLFANNDYNEISDSTMKLGPKNLLPNVQGISLFPSLLGACPHPRYMDYFATAHSQHGLIQLINTYEKDSNSIPIQLGMPIVDFCWHQDGSHLAIATEGSVLLTRLMGFTRL 378B5289C494AB3D nu120_schpo Nucleoporin nup120 Nuclear pore protein nup120 nup120 SPBC3B9.16c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNELKHAVVPIDLQSFCLEGTLALWVPALENDSEDDSEAIETADDNEKLFKKECVAYDAGVYTSNKSKGSQTLRWSIFQNRTLTIFDVSLNSKKEPLSKFNVKIHFPSNVMKDGVAFSFSEHSDTTIIYAITHARVLYYIRLSKTWFQLPDARLDDDWCLCYRPISFLNQKPDLMAAISTSEICVSFFNGGLTKIILNPKDASHYEQHIDDSSYLFSLKKYLSLQAFKADYRSPNTIISMIFLSTYNVLVMLSLDYKLKVLDLSTNQCVETIELSQTILPLQSFPYLTSDHTTNSFIALYYPDNSHGSFSIYKLNANAHSFKLNVVIEKGIIPPSLPDDEFIPWMLSDFQLISSEGSQSKFLLIIAWKSNLNTVIQKCNLSLDQDESFSCVWSHSLDSFSLIEKTFFDVPTNMSSGDISEIWLQHIFAHNTSIESIQVALLSFQNSSSQVSKNKLDKFGALTISELKNAVLSSIVSTIQIEPNSDLTGYDYYEYKRLLYNEWERFAKLVAYLDHFGDEILSINFDPSNAVTYINYANKVAFIRDPYLIESFDEEPLTKLISSLETDDPSLIEGYQILDLGRSLHSCMSFSTLSEIRYSLRELVQDLPSYSLFDTLWVFYDKHIYPNVDPDYISTLIDTLVSLENPMRDIDSLIQRLRSFDIYNHSAQSPSLFLCASVARVLDSILKKFQVSIEGFIFLLSLITSQQDYELQSKFAGCDKLFLSLLEDWRLVSFLLENSALLLEKFEEEDVDSTNCNLNTMEALASVNTALQFFSALNYSECFSESQISPLHATVISSLSAIFIRDDTENDLVTELVEKLFLFKQYNACMQLIGWLNSDPIAVYLKALIYLKSKEAVKAVRCFKTTSLVLYSHTSQFAVLREFQEIAEKYHHQNLLSCYYLHLSKKLFEESAYIDALEFSLLADASKETDDEDLSIAITHETLKTACAAGKFDAAHVALMVLSTTPLKKSCLLDFVNQLTKQGKINQLLNYSMPTLRQDVDNLLERKAFQMINVESQPCWYNILFSWRYKHQNYRDAAAIIYEKLSRYISTTELIGKKERTFIIEHYLIVLNTLELLPKEDTWILVTDMSVDKEPDPNFLPQKLLTLDAIVAEYHLQLKDVAVQVTAEMSSAMNIDL 0D8E7A0839FA6FFE 0D8E7A0839FA6FFE spnup120-spnup37-1 3 5 unspecified role unspecified role unspecified role unspecified role Region 1-950 Region 1-950 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction spnup120-spnup37-2 1 5 unspecified role unspecified role unspecified role unspecified role Region 1-950 Region 1-950 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction spnup120-spnup37-3 2 5 unspecified role unspecified role unspecified role unspecified role Region 1-950 Region 1-950 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 4 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction