DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Internally deleted human tRNA synthetase suggests evolutionary pressure for repurposing. Internally deleted human tRNA synthetase suggests evolutionary pressure for repurposing. Structure (London, England : 1993) 2012 imex curation Xu Z., Wei Z., Zhou JJ., Ye F., Lo WS., Wang F., Lau CF., Wu J., Nangle LA., Chiang KP., Yang XL., Zhang M., Schimmel P. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Xu Z., Wei Z., Zhou JJ., Ye F., Lo WS., Wang F., Lau CF., Wu J., Nangle LA., Chiang KP., Yang XL., Zhang M., Schimmel P. DIP original experiment: DIP-250536X Structure (London, England : 1993) 2012 IMEx Internally deleted human tRNA synthetase suggests evolutionary pressure for repurposing. Internally deleted human tRNA synthetase suggests evolutionary pressure for repurposing. Internally deleted human tRNA synthetase suggests evolutionary pressure for repurposing. Structure (London, England : 1993) 2012 imex curation Xu Z., Wei Z., Zhou JJ., Ye F., Lo WS., Wang F., Lau CF., Wu J., Nangle LA., Chiang KP., Yang XL., Zhang M., Schimmel P. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Xu Z., Wei Z., Zhou JJ., Ye F., Lo WS., Wang F., Lau CF., Wu J., Nangle LA., Chiang KP., Yang XL., Zhang M., Schimmel P. DIP original experiment: DIP-250535X Structure (London, England : 1993) 2012 IMEx Internally deleted human tRNA synthetase suggests evolutionary pressure for repurposing. hars1_human Histidine--tRNA ligase, cytoplasmic HARS1 HARS HRS Histidyl-tRNA synthetase protein protein human Homo sapiens Human MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC DIP protein P12081 original sequence version: 134 65D8BB71CE79B1FF hars-1 2 3 unspecified role unspecified role unspecified role unspecified role Region 61-398 Region 61-398 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 54-506 Region 54-506 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction hars-2 1 3 unspecified role unspecified role unspecified role unspecified role Region 54-506 Region 54-506 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction