DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251792X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251793X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251790X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251791X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Spåhr H., Habermann B., Gustafsson CM., Larsson NG., Hallberg BM. DIP original experiment: DIP-251789X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. mtef4_human Transcription termination factor 4, mitochondrial Mitochondrial transcription termination factor 4 mTERF domain-containing protein 2 MTERF4 MTERFD2 HSPC096 protein protein human Homo sapiens Human MAAFGRQVLDWHRLIPLTWACMARQTPHLGEQRRTTASLLRKLTTASNGGVIEELSCVRSNNYVQEPECRRNLVQCLLEKQGTPVVQGSLELERVMSSLLDMGFSNAHINELLSVRRGASLQQLLDIISEFILLGLNPEPVCVVLKKSPQLLKLPIMQMRKRSSYLQKLGLGEGKLKRVLYCCPEIFTMRQQDINDTVRLLKEKCLFTVQQVTKILHSCPSVLREDLGQLEYKFQYAYFRMGIKHPDIVKSEYLQYSLTKIKQRHIYLERLGRYQTPDKKGQTQIPNPLLKDILRVSEAEFLARTACTSVEEFQVFKKLLAREEEESESSTSDDKRASLDEDEDDDDEEDNDEDDNDEDDDDEDDDEAEDNDEDEDDDEEE DIP protein Q7Z6M4 original sequence version: 71 DIP protein Q7Z6M4 original sequence version: 68 45592F93558EE190 q96cb9-1 5-methylcytosine rRNA methyltransferase NSUN4 NSUN4 NOL1/NOP2/Sun domain family member 4 5-methylcytosine tRNA methyltransferase NSUN4 protein protein human Homo sapiens Human MAALTLRGVRELLKRVDLATVPRRHRYKKKWAATEPKFPAVRLALQNFDMTYSVQFGDLWPSIRVSLLSEQKYGALVNNFAAWDHVSAKLEQLSAKDFVNEAISHWELQSEGGQSAAPSPASWACSPNLRCFTFDRGDISRFPPARPGSLGVMEYYLMDAASLLPVLALGLQPGDIVLDLCAAPGGKTLALLQTGCCRNLAANDLSPSRIARLQKILHSYVPEEIRDGNQVRVTSWDGRKWGELEGDTYDRVLVDVPCTTDRHSLHEEENNIFKRSRKKERQILPVLQVQLLAAGLLATKPGGHVVYSTCSLSHLQNEYVVQGAIELLANQYSIQVQVEDLTHFRRVFMDTFCFFSSCQVGELVIPNLMANFGPMYFCKMRRLT DIP protein Q96CB9-1 original sequence version: 73 DIP protein Q96CB9-1 original sequence version: 70 mterf4-nsun4-1 5 7 unspecified role unspecified role prey prey Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role bait bait Region 48-381 Region 48-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association nsun4-1 2 7 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96CB9-1:p.[Val65Arg;Arg136Ala;Ile139Arg;Arg141Ala] V65R/I139R/R136A/R141A mutation mutation certain certain sequence position certain certain certain sequence position certain V R certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain I R certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'V65R/I139R/R136A/R141A'. Wed May 02 19:17:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65arg,arg136ala,ile139arg,arg141ala' Tue Jun 30 18:28:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val65Arg;Arg136Ala;Ile139Arg;Arg141Ala]' ecolx Escherichia coli direct interaction direct interaction mterf4-nsun4-2 4 7 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 48-381 Region 48-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mterf4-nsun4-3 3 7 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 48-381 Region 48-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction mterf4-nsun4-4 1 7 unspecified role unspecified role unspecified role unspecified role Region 26-384 Region 26-384 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96CB9-1:p.[Val65Arg;Arg136Ala;Ile139Arg;Arg141Ala] V65R/I139R/R136A/R141A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V R certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain I R certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'V65R/I139R/R136A/R141A'. Wed May 02 15:06:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val65arg,arg136ala,ile139arg,arg141ala' Tue Jun 30 19:52:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val65Arg;Arg136Ala;Ile139Arg;Arg141Ala]' ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 48-381 Region 48-381 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction