DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu DNA minor groove sensing and widening by the CCAAT-binding complex. DNA minor groove sensing and widening by the CCAAT-binding complex. Structure (London, England : 1993) 2012 imex curation Huber EM., Scharf DH., Hortschansky P., Groll M., Brakhage AA. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Huber EM., Scharf DH., Hortschansky P., Groll M., Brakhage AA. DIP original experiment: DIP-250986X Structure (London, England : 1993) 2012 IMEx DNA minor groove sensing and widening by the CCAAT-binding complex. DNA minor groove sensing and widening by the CCAAT-binding complex. DNA minor groove sensing and widening by the CCAAT-binding complex. Structure (London, England : 1993) 2012 imex curation Huber EM., Scharf DH., Hortschansky P., Groll M., Brakhage AA. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Huber EM., Scharf DH., Hortschansky P., Groll M., Brakhage AA. DIP original experiment: DIP-250985X Structure (London, England : 1993) 2012 IMEx DNA minor groove sensing and widening by the CCAAT-binding complex. DNA minor groove sensing and widening by the CCAAT-binding complex. DNA minor groove sensing and widening by the CCAAT-binding complex. Structure (London, England : 1993) 2012 imex curation Huber EM., Scharf DH., Hortschansky P., Groll M., Brakhage AA. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Huber EM., Scharf DH., Hortschansky P., Groll M., Brakhage AA. DIP original experiment: DIP-250987X Structure (London, England : 1993) 2012 IMEx DNA minor groove sensing and widening by the CCAAT-binding complex. DNA minor groove sensing and widening by the CCAAT-binding complex. DNA minor groove sensing and widening by the CCAAT-binding complex. Structure (London, England : 1993) 2012 imex curation Huber EM., Scharf DH., Hortschansky P., Groll M., Brakhage AA. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Huber EM., Scharf DH., Hortschansky P., Groll M., Brakhage AA. DIP original experiment: DIP-250984X Structure (London, England : 1993) 2012 IMEx DNA minor groove sensing and widening by the CCAAT-binding complex. q00735_emend hapC protein protein emend Emericella nidulans Aspergillus nidulans MSSTSPSKEPEVEQEPHSGEEHEQMDKEQDNQTQGQGEFEVKEQDRWLPIANVARIMKLALPENAKIAKEAKECMQECVSEFISFITSEASEKCQQEKRKTVNGEDILFAMTSLGFENYAEALKIYLSKYRETQSARGEHQNRPPSSGYAGGDPSAELPAALQSCVPGVLQLEAPSPKPLIMQAAL DIP protein Q00735 original sequence version: 66 p87092_emend hapE protein protein emend Emericella nidulans Aspergillus nidulans MEQAQQTSGQQQGRQQPVYDTRQGGHYGASAALAAQGFAPVAELYTGTWANVNQGLQGTARDILTTYWQHVINHLESDNHDYKIHQLPLARIKKVMKADPEVKMISAEAPILFAKGCDVFITELTMRAWIHAEDNKRRTLQRSDIAAALSKSDMFDFLIDIVPREEATSHAKRSSQSAGAPAGPGGPTAAGQLPQTQHGVQHHPHHMAPPDYGALGQHPLQDQEYRQQTMYGGAVQSDPTAAYAQPQTQMFEGMYTAYPHLPPQQ DIP protein P87092 original sequence version: 66 p87249_emend Transcriptional activator HAP2 hapB protein protein emend Emericella nidulans Aspergillus nidulans MEYSPQYQQQHGQHPHPASHMTGAYPTAPQNPGSALGSMTSPTNPQTHIQQAHPTHQASPVVPSQSHYQQAQNAPGSMHQQMNFPQSYGVTTAMPPQPYGISPTQAAAMATAAASGQFYPTHGDSIAGQMAQGPRGSPRMAGVPVKTDRNPRSPPQIPGQMPPMGSQVPLTPTAQMQQRRMSHVGSPHVQNAQPVMNHVGRPSVPPPMPPPQHPVQQTQTSPDMMPSGAEESPLYVNAKQFHRILKRRVARQKLEEQLRLTSKGRKPYLHESRHNHAMRRPRGPGGRFLTADEVAAMEKKNAAGGQENADPNASKAVSDSSPASQKRKASDGNNENPNSAKKAKTGAQKASNNADESEAESGGPSDEDG DIP protein P87249 original sequence version: 58 hapb-hapc-1 3 5 unspecified role unspecified role prey prey Region 42-132 Region 42-132 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role prey prey Region 47-164 Region 47-164 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 231-269 Region 231-269 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli association association hapb-hapc-2 4 5 unspecified role unspecified role unspecified role unspecified role Region 42-132 Region 42-132 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 47-164 Region 47-164 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 231-293 Region 231-293 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction hapb-hapc-3 1 5 unspecified role unspecified role prey prey Region 42-132 Region 42-132 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role prey prey Region 47-164 Region 47-164 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role bait bait Region 231-280 Region 231-280 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli association association hapb-hapc-4 2 5 unspecified role unspecified role unspecified role unspecified role Region 42-132 Region 42-132 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 47-164 Region 47-164 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 231-269 Region 231-269 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association