UniProt UniProt http://www.uniprot.org ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response. ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response. Genes & development 2012 imex curation Weston R., Peeters H., Ahel D. Interactions involving transfected YFP-Pol(eta) omitted as the plasmid origin cannot be traced (CoIP and immunofluorescence exp) dahel@picr.man.ac.uk 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Weston R., Peeters H., Ahel D. Interactions involving transfected YFP-Pol(eta) omitted as the plasmid origin cannot be traced (CoIP and immunofluorescence exp) dahel@picr.man.ac.uk Accepted 2017-MAR-29 AT 10:36 BST AT 10:36 BST by MGT Genes & development 2012 imex curation ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response. ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response. Genes & development 2012 imex curation Weston R., Peeters H., Ahel D. Interactions involving transfected YFP-Pol(eta) omitted as the plasmid origin cannot be traced (CoIP and immunofluorescence exp) dahel@picr.man.ac.uk 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Weston R., Peeters H., Ahel D. Interactions involving transfected YFP-Pol(eta) omitted as the plasmid origin cannot be traced (CoIP and immunofluorescence exp) dahel@picr.man.ac.uk Accepted 2017-MAR-29 AT 10:36 BST AT 10:36 BST by MGT Genes & development 2012 imex curation ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response. ZRANB3 is a structure-specific ATP-dependent endonuclease involved in replication stress response. Genes & development 2012 imex curation Weston R., Peeters H., Ahel D. Interactions involving transfected YFP-Pol(eta) omitted as the plasmid origin cannot be traced (CoIP and immunofluorescence exp) dahel@picr.man.ac.uk 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference confocal microscopy confocal microscopy immunostaining immunostaining Weston R., Peeters H., Ahel D. Interactions involving transfected YFP-Pol(eta) omitted as the plasmid origin cannot be traced (CoIP and immunofluorescence exp) dahel@picr.man.ac.uk Accepted 2017-MAR-29 AT 10:36 BST AT 10:36 BST by MGT Genes & development 2012 imex curation zrab3_human DNA annealing helicase and endonuclease ZRANB3 ZRANB3 Annealing helicase 2 Zinc finger Ran-binding domain-containing protein 3 protein protein human Homo sapiens Human MPRVHNIKKSLTPHISCVTNESDNLLDFLPDRLRAKLLPFQKDGIIFALKRNGRCMVADEMGLGKTIQAIGITYFYKEEWPLLIVVPSSLRYPWTEEIEKWIPELSPEEINVIQNKTDVRRMSTSKVTVLGYGLLTADAKTLIDALNNQNFKVVIVDESHYMKSRNATRSRILLPIVQKARRAILLTGTPALGRPEELFMQIEALFPQKFGRWTDYAKRYCNAHIRYFGKRPQWDCRGASNLNELHQLLSDIMIRRLKTEVLTQLPPKVRQRIPFDLPSAAAKELNTSFEEWEKIMRTPNSGAMETVMGLITRMFKQTAIAKAGAVKDYIKMMLQNDSLKFLVFAHHLSMLQACTEAVIENKTRYIRIDGSVSSSERIHLVNQFQKDPDTRVAILSIQAAGQGLTFTAASHVVFAELYWDPGHIKQAEDRAHRIGQCSSVNIHYLIANGTLDTLMWGMLNRKAQVTGSTLNGRKEKIQAEEGDKEKWDFLQFAEAWTPNDSSEELRKEALFTHFEKEKQHDIRSFFVPQPKKRQLMTSCDESKRFREENTVVSSDPTKTAARDIIDYESDVEPETKRLKLAASEDHCSPSEETPSQSKQIRTPLVESVQEAKAQLTTPAFPVEGWQCSLCTYINNSELPYCEMCETPQGSAVMQIDSLNHIQDKNEKDDSQKDTSKKVQTISDCEKQALAQSEPGQLADSKEETPKIEKEDGLTSQPGNEQWKSSDTLPVYDTLMFCASRNTDRIHIYTKDGKQMSCNFIPLDIKLDLWEDLPASFQLKQYRSLILRFVREWSSLTAMKQRIIRKSGQLFCSPILALEEITKQQTKQNCTKRYITKEDVAVASMDKVKNVGGHVRLITKESRPRDPFTKKLLEDGACVPFLNPYTVQADLTVKPSTSKGYLQAVDNEGNPLCLRCQQPTCQTKQACKANSWDSRFCSLKCQEEFWIRSNNSYLRAKVFETEHGVCQLCNVNAQELFLRLRDAPKSQRKNLLYATWTSKLPLEQLNEMIRNPGEGHFWQVDHIKPVYGGGGQCSLDNLQTLCTVCHKERTARQAKERSQVRRQSLASKHGSDITRFLVKK 7B65AB9568B15AAE mcm7_human DNA replication licensing factor MCM7 MCM7 CDC47 MCM2 CDC47 homolog P1.1-MCM3 protein protein human Homo sapiens Human MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDVKKALLLLLVGGVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLRDSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNARCSILAAANPAYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYVHQHSRQPPSQFEPLDMKLMRRYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATYTSARTLLAILRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV DIP protein P33993 original sequence version: 161 DIP protein P33993 original sequence version: 124 330A1DEFAEFBFB88 mcm4_human DNA replication licensing factor MCM4 MCM4 CDC21 CDC21 homolog P1-CDC21 protein protein human Homo sapiens Human MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQSPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKEEENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIPEMQEAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQESPEDMPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHYRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLLLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKTVRLL DIP protein P33991 original sequence version: 121 DIP protein P33991 original sequence version: 165 DIP protein P33991 original sequence version: 168 96D9CA2A7D88D015 mcm3_human DNA replication licensing factor MCM3 MCM3 DNA polymerase alpha holoenzyme-associated protein P1 RLF subunit beta p102 P1-MCM3 protein protein human Homo sapiens Human MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI DIP protein P25205 original sequence version: 135 DIP protein P25205 original sequence version: 179 C967C068B7090558 pcna_human Proliferating cell nuclear antigen PCNA Cyclin protein protein human Homo sapiens Human MFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLALVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRDLSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYYLAPKIEDEEGS DIP protein P12004 original sequence version: 200 DIP protein P12004 original sequence version: 128 DIP protein P12004 original sequence version: 139 DIP protein P12004 original sequence version: 185 DIP protein P12004 original sequence version: 102 DIP protein P12004 original sequence version: 105 DIP protein P12004 original sequence version: 158 E6F08E7EDBC48B00 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG zranb3-mcm3-1 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 1B pcna-zranb3-1 3 8 unspecified role unspecified role neutral component neutral component 4 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5FWF4:p.[Gln519Ala;Phe525Ala;Phe526Ala;Tyr632Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:58:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln519ala,phe525ala,phe526ala,tyr632ala' Tue Jun 30 20:00:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln519Ala;Phe525Ala;Phe526Ala;Tyr632Ala]' colocalization colocalization 5C- F Colocalize in DNA damage-induced foci pcna-zranb3-2 2 8 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 tag fluorescence tag visualisation by fluorescence tag fluorescence western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range range range Q5FWF4:p.[Gln519Ala;Phe525Ala;Phe526Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 14:43:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln519ala,phe525ala,phe526ala' Tue Jun 30 18:52:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln519Ala;Phe525Ala;Phe526Ala]' chemical synthesis Chemical synthesis (Chemical synthesis) physical association physical association 2B zranb3-mcm3-2 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range PIP-box gln519ala,phe525ala,phe526ala construct abolishes association of PCNA but has no effect on association with MCM3 and MCM4 association association 2C ubiq_human-zranb3-1 3 9 unspecified role unspecified role neutral component neutral component Used antibody recognizes conjugated but not free ubiquitin 4 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 2F pcna-zranb3-3 3 8 unspecified role unspecified role neutral component neutral component 4 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range hnh sufficient to bind sufficient binding region sufficient to bind range range range range colocalization colocalization 5D, supplemental fig 5C Colocalize in untreated and in DNA damage-induced foci with comparable efficiencies pcna-zranb3-4 3 8 unspecified role unspecified role neutral component neutral component 4 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5FWF4:p.[Gln519Ala;Phe525Ala;Phe526Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain Q A Tue May 09 10:48:57 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'trp519ala,phe525ala,phe526ala' Wed May 02 20:19:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln519ala,phe525ala,phe526ala' Tue Jun 30 17:06:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln519Ala;Phe525Ala;Phe526Ala]' colocalization colocalization 5D, supplemental fig 5C Colocalize in DNA damage-induced foci pcna-zranb3-5 3 8 unspecified role unspecified role neutral component neutral component 4 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n-terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5FWF4:p.Tyr632Ala mutation mutation certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 18:15:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr632ala' Tue Jun 30 16:17:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr632Ala' colocalization colocalization 5D, supplemental fig 5C Colocalize in DNA damage-induced foci zranb3-mcm3-3 1 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range PIP-box gln519ala,phe525ala,phe526ala construct abolishes association of PCNA but has no effect on association with MCM3 and MCM4 physical association physical association supplemental fig 2B UV radiation does not change the interaction zranb3-pcna-1 1 6 unspecified role unspecified role prey prey 4 tag fluorescence tag visualisation by fluorescence tag fluorescence western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5FWF4:p.[Gln519Ala;Phe525Ala;Phe526Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain Q A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:49:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln519ala,phe525ala,phe526ala' Tue Jun 30 17:37:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln519Ala;Phe525Ala;Phe526Ala]' 7 unspecified role unspecified role prey prey association association 2C-2