IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Sho1p connects the plasma membrane with proteins of the cytokinesis network through multiple isomeric interaction states. Sho1p connects the plasma membrane with proteins of the cytokinesis network through multiple isomeric interaction states. Journal of cell science 2012 imex curation Labedzka K., Tian C., Nussbaumer U., Timmermann S., Walther P., Müller J., Johnsson N. nils.johnsson@uni-ulm.de 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Labedzka K., Tian C., Nussbaumer U., Timmermann S., Walther P., Müller J., Johnsson N. nils.johnsson@uni-ulm.de Accepted 2017-MAY-18 AT 14:03 BST AT 14:03 BST by MGT Journal of cell science 2012 imex curation Sho1p connects the plasma membrane with proteins of the cytokinesis network through multiple isomeric interaction states. Sho1p connects the plasma membrane with proteins of the cytokinesis network through multiple isomeric interaction states. Journal of cell science 2012 imex curation Labedzka K., Tian C., Nussbaumer U., Timmermann S., Walther P., Müller J., Johnsson N. nils.johnsson@uni-ulm.de 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Labedzka K., Tian C., Nussbaumer U., Timmermann S., Walther P., Müller J., Johnsson N. nils.johnsson@uni-ulm.de Accepted 2017-MAY-18 AT 14:03 BST AT 14:03 BST by MGT Journal of cell science 2012 imex curation Reduced to Supp Table 1 only, as discussed.; Looked at pdf, corrected according to that. bud14_yeast Bud site selection protein 14 FUN2 YAR014C BUD14 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNKEEHVDETSASGVKEVSSIAARHDNGYAPSLITSTSGMDSFQSHALLNDPTLIEDYSDIINNRPTSGSKLTLGNEDSESMGGSVVVTPTSNKSSPFNSKLNILSNAAEKGHDVLRNRDDDKELEEENVEKHMHSNSKRDQRHYKENSSELPDSYDYSDSEFEDNLERRLQEIETDSVDSADKDEVHFSVNNTMNPDVDDFSDGLKYAISEDEDEEENYSDDDDFDRKFQDSGFQGEKDDLEEENDDYQPLSPPRELDPDKLYALYAFNGHDSSHCQLGQDEPCILLNDQDAYWWLVKRITDGKIGFAPAEILETFPERLARLNCWKNENMSSQSVASSDSKDDSISSGNKNQSDAESIIPTPALNGYGKGNKSVSFNDVVGYADRFIDDAIEDTSLDSNDDGGEGNGQSYDDDVDNDKETKVTHRDEYTEAKLNFGKFQDDDTSDVVSDVSFSTSLNTPLNVKKVRRQDNKNESEPKTSSSKDREDDYNANRYVGQEKSEPVDSDYDTDLKKVFEAPRMPFANGMAKSDSQNSLSTIGEFSPSSSEWTNESPSTPIVEESSSIPSSRAIKDISQYIHAKSKIEETTNVENTEGQIQASLGSSGGMANQTDAEQPKEELEKHHSTPEEEKQSTLSLHSSSEEDFYMDEQRAVSSASINSSLSGSRALSNTNMSDPASKPNSLVQHLYAPVFDRMDVLMKQLDEIIRK F123511A4C592EB4 F123511A4C592EB4 slt2_yeast Mitogen-activated protein kinase SLT2/MPK1 YHR030C SLT2 MPK1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MADKIERHTFKVFNQDFSVDKRFQLIKEIGHGAYGIVCSARFAEAAEDTTVAIKKVTNVFSKTLLCKRSLRELKLLRHFRGHKNITCLYDMDIVFYPDGSINGLYLYEELMECDMHQIIKSGQPLTDAHYQSFTYQILCGLKYIHSADVLHRDLKPGNLLVNADCQLKICDFGLARGYSENPVENSQFLTEYVATRWYRAPEIMLSYQGYTKAIDVWSAGCILAEFLGGKPIFKGKDYVNQLNQILQVLGTPPDETLRRIGSKNVQDYIHQLGFIPKVPFVNLYPNANSQALDLLEQMLAFDPQKRITVDEALEHPYLSIWHDPADEPVCSEKFEFSFESVNDMEDLKQMVIQEVQDFRLFVRQPLLEEQRQLQLQQQQQQQQQQQQQQQQPSDVDNGNAAASEENYPKQMATSNSVAPQQESFGIHSQNLPRHDADFPPRPQESMMEMRPATGNTADIPPQNDNGTLLDLEKELEFGLDRKYF 559A3E0D3EBDE5F9 559A3E0D3EBDE5F9 pbs2_yeast MAP kinase kinase PBS2 PBS2 HOG4 SFS4 SSK4 YJL128C J0699 Polymyxin B resistance protein 2 Suppressor of fluoride sensitivity 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEDKFANLSLHEKTGKSSIQLNEQTGSDNGSAVKRTSSTSSHYNNINADLHARVKAFQEQRALKRSASVGSNQSEQDKGSSQSPKHIQQIVNKPLPPLPVAGSSKVSQRMSSQVVQASSKSTLKNVLDNQETQNITDVNINIDTTKITATTIGVNTGLPATDITPSVSNTASATHKAQLLNPNRRAPRRPLSTQHPTRPNVAPHKAPAIINTPKQSLSARRGLKLPPGGMSLKMPTKTAQQPQQFAPSPSNKKHIETLSNSKVVEGKRSNPGSLINGVQSTSTSSSTEGPHDTVGTTPRTGNSNNSSNSGSSGGGGLFANFSKYVDIKSGSLNFAGKLSLSSKGIDFSNGSSSRITLDELEFLDELGHGNYGNVSKVLHKPTNVIMATKEVRLELDEAKFRQILMELEVLHKCNSPYIVDFYGAFFIEGAVYMCMEYMDGGSLDKIYDESSEIGGIDEPQLAFIANAVIHGLKELKEQHNIIHRDVKPTNILCSANQGTVKLCDFGVSGNLVASLAKTNIGCQSYMAPERIKSLNPDRATYTVQSDIWSLGLSILEMALGRYPYPPETYDNIFSQLSAIVDGPPPRLPSDKFSSDAQDFVSLCLQKIPERRPTYAALTEHPWLVKYRNQDVHMSEYITERLERRNKILRERGENGLSKNVPALHMGGL 9BC3435BDAFE8019 9BC3435BDAFE8019 nba1_yeast Protein NBA1 YOL070C NBA1 NAP1 and bud neck-associated protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEEREENGISRATLNTQRLSAMIDSLNNEKDDRLFPSPTTTRTMITEEKADQSDVFKPPSRLLRSPAGDVSLPPGDNRSSMISNYSGIIQEGVEVSYVVKNRQQTQERRTSKDSNSLYSLKEPVSKNELPSLPMLPSEATLTKHLSDNQSTKSNTNADEIVIKPVTNAKPVGRFNSNTSKKVEGRGSLKLLSSPLRQEKVMRSSIGSGNLASESGSSTYNTKFHQSIQEQLEEEEEGNVSDKLSIVSSVIPELYTTTNEAPKAINPIRSETNDYNPTIPPRSKDRPRSRLFIEEGDGEGDLLTEEILPTPVQPGGHYKNSSQISTVSEQKSESYYSAATSMPPEEETYLTRPLPSTPNEDSRVTSNLKRDDTLKAIHDRANHTSTSTNKQDDDMYEDIIEETPKKTKLKKDTKKKLNKKKSVKELRSFDIDTLNQLLSVTKGTLIGSEFAQLGMKIEEKRALERLVDSLSRLTADMVLDPDRYEEGLKRLDKATKALEGF 8128246D00B9C03D 8128246D00B9C03D kapa_yeast cAMP-dependent protein kinase type 1 TPK1 PKA1 SRA3 YJL164C J0541 CDC25-suppressing protein kinase PK-25 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTEEQNGGGQKSLDDRQGEESQKGETSERETTATESGNESKSVEKEGGETQEKPKQPHVTYYNEEQYKQFIAQARVTSGKYSLQDFQILRTLGTGSFGRVHLIRSRHNGRYYAMKVLKKEIVVRLKQVEHTNDERLMLSIVTHPFIIRMWGTFQDAQQIFMIMDYIEGGELFSLLRKSQRFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFGFAKYVPDVTYTLCGTPDYIAPEVVSTKPYNKSIDWWSFGILIYEMLAGYTPFYDSNTMKTYEKILNAELRFPPFFNEDVKDLLSRLITRDLSQRLGNLQNGTEDVKNHPWFKEVVWEKLLSRNIETPYEPPIQQGQGDTSQFDKYPEEDINYGVQGEDPYADLFRDF D4E5C30D7F11458B D4E5C30D7F11458B cdc3_yeast Cell division control protein 3 CDC3 L8543.7 YLR314C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLKEEQVSIKQDPEQEERQHDQFNDVQIKQESQDHDGVDSQYTNGTQNDDSERFEAAESDVKVEPGLGMGITSSQSEKGQVLPDQPEIKFIRRQINGYVGFANLPKQWHRRSIKNGFSFNLLCVGPDGIGKTTLMKTLFNNDDIEANLVKDYEEELANDQEEEEGQGEGHENQSQEQRHKVKIKSYESVIEENGVKLNLNVIDTEGFGDFLNNDQKSWDPIIKEIDSRFDQYLDAENKINRHSINDKRIHACLYFIEPTGHYLKPLDLKFMQSVYEKCNLIPVIAKSDILTDEEILSFKKTIMNQLIQSNIELFKPPIYSNDDAENSHLSERLFSSLPYAVIGSNDIVENYSGNQVRGRSYPWGVIEVDNDNHSDFNLLKNLLIKQFMEELKERTSKILYENYRSSKLAKLGIKQDNSVFKEFDPISKQQEEKTLHEAKLAKLEIEMKTVFQQKVSEKEKKLQKSETELFARHKEMKEKLTKQLKALEDKKKQLELSINSASPNVNHSPVPTKKKGFLR DCCD4C8C16AA5E25 DCCD4C8C16AA5E25 cdc14_yeast Tyrosine-protein phosphatase CDC14 CDC14 OAF3 YFR028C protein protein yeast Saccharomyces cerevisiae Baker's yeast MRRSVYLDNTIEFLRGRVYLGAYDYTPEDTDELVFFTVEDAIFYNSFHLDFGPMNIGHLYRFAVIFHEILNDPENANKAVVFYSSASTRQRANAACMLCCYMILVQAWTPHQVLQPLAQVDPPFMPFRDAGYSNADFEITIQDVVYGVWRAKEKGLIDLHSFNLESYEKYEHVEFGDFNVLTPDFIAFASPQEDHPKGYLATKSSHLNQPFKSVLNFFANNNVQLVVRLNSHLYNKKHFEDIGIQHLDLIFEDGTCPDLSIVKNFVGAAETIIKRGGKIAVHCKAGLGRTGCLIGAHLIYTYGFTANECIGFLRFIRPGMVVGPQQHWLYLHQNDFREWKYTTRISLKPSEAIGGLYPLISLEEYRLQKKKLKDDKRVAQNNIEGELRDLTMTPPSNGHGALSARNSSQPSTANNGSNSFKSSAVPQTSPGQPRKGQNGSNTIEDINNNRNPTSHANRKVVIESNNSDDESMQDTNGTSNHYPKVSRKKNDISSASSSRMEDNEPSATNINNAADDTILRQLLPKNRRVTSGRRTTSAAGGIRKISGSIKK 4EB3985DFA3FD823 4EB3985DFA3FD823 sla1_yeast Actin cytoskeleton-regulatory complex protein SLA1 YBL0321 YBL007C SLA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTVFLGIYRAVYAYEPQTPEELAIQEDDLLYLLQKSDIDDWWTVKKRVIGSDSEEPVGLVPSTYIEEAPVLKKVRAIYDYEQVQNADEELTFHENDVFDVFDDKDADWLLVKSTVSNEFGFIPGNYVEPENGSTSKQEQAPAAAEAPAATPAAAPASAAVLPTNFLPPPQHNDRARMMQSKEDQAPDEDEEGPPPAMPARPTATTETTDATAAAVRSRTRLSYSDNDNDDEEDDYYYNSNSNNVGNHEYNTEYHSWNVTEIEGRKKKKAKLSIGNNKINFIPQKGTPHEWSIDKLVSYDNEKKHMFLEFVDPYRSLELHTGNTTTCEEIMNIIGEYKGASRDPGLREVEMASKSKKRGIVQYDFMAESQDELTIKSGDKVYILDDKKSKDWWMCQLVDSGKSGLVPAQFIEPVRDKKHTESTASGIIKSIKKNFTKSPSRSRSRSRSKSNANASWKDDELQNDVVGSAAGKRSRKSSLSSHKKNSSATKDFPNPKKSRLWVDRSGTFKVDAEFIGCAKGKIHLHKANGVKIAVAADKLSNEDLAYVEKITGFSLEKFKANDGSSSRGTDSRDSERERRRRLKEQEEKERDRRLKERELYELKKARELLDEERSRLQEKELPPIKPPRPTSTTSVPNTTSVPPAESSNNNNSSNKYDWFEFFLNCGVDVSNCQRYTINFDREQLTEDMMPDINNSMLRTLGLREGDIVRVMKHLDKKFGRENIASIPTNATGNMFSQPDGSLNVATSPETSLPQQLLPQTTSPAQTAPSTSAETDDAWTVKPASKSESNLLSKKSEFTGSMQDLLDLQPLEPKKAAASTPEPNLKDLEPVKTGGTTVPAAPVSSAPVSSAPAPLDPFKTGGNNILPLSTGFVMMPMITGGDMLPMQRTGGFVVPQTTFGMQSQVTGGILPVQKTGNGLIPISNTGGAMMPQTTFGAAATVLPLQKTGGGLIPIATTGGAQFPQTSFNVQGQQQLPTGSILPVQKTANGLISANTGVSMPTVQRTGGTMIPQTSFGVSQQLTGGAMMTQPQNTGSAMMPQTSFNAVPQITGGAMMPQTSFNALPQVTGGAMMPLQRTGGALNTFNTGGAMIPQTSFSSQAQNTGGFRPQSQFGLTLQKTGGIAPLNQNQFTGGAMNTLSTGGVLQQQQPQTMNTFNTGGVMQELQMMTTFNTGGAMQQPQMMNTFNTDGIMQQPQMMNTFNTGGAMQQPQQQALQNQPTGFGFGNGPQQSRQANIFNATASNPFGF 7FD85AA776407624 7FD85AA776407624 cyk3_yeast Cytokinesis protein 3 CYK3 YDL117W protein protein yeast Saccharomyces cerevisiae Baker's yeast MATNLTSLKPPFKVKARYGWSGQTKGDLGFLEGDIMEVTRIAGSWFYGKLLRNKKCSGYFPHNFVILLEERLNSSTENGRQPSKIVESFEKSNKVVIPPVPSRYSDERPRPKKKLSSSMPNSPKKPVDSLTKARKAKSKEMVNEKNIYNTQSSRHHNNSAPNLPLASHSKPQVRNFEESMNNPLPPLPPLPDLDNMRKTDKRAPKKSYSANDLHMARSSREYNYYKDNQKFYDGFIPEKRYSLEEDSISSGLFSNSQYLNDSACSSENSFALMSDFSATSAGSFARHKYAQSFSDSLQRSQNANGCSTKINDSQEFGDSNASSRNGKMGDILRKIIIPKRNTNIYSSSVSSPKSPKAYPKLPDIQNLNLSATPDEARDWIAVKCHLNRARTLTKYDKHPRYMRALEENRDLILHPQDSIYNGLNTNEVKGNTKPGLVDVELAELNIEYIDKMTWKRCIRDGTMTLDSWAQTTFSARYSTVLEKLRGIYIFCTEMFALTDDNGTSDFSAEPQNLEKILYRKHCTPYELTWLFKKLANSLGITCEIVIGFLKTPSAINWEFKYNHCWLRILVNKEWRFIDVILGNVTNPIHEFVNNRKIKKAENSYFLMAPLEMIYTHIPPREFEQHIVPSIDQLSALYLPLVFPSFFKNELKLYKFSTALSFLEDSEIYECSLEIPNDVEVFASVVIPTDNEEASSAYRNMELALTQIKKQKAESGRRIALIKAVLPPNVNKGSLYIHSGVRGTQTSIANIHPLSMMVPLTHKGSNMKYEFVIKIPSESIQKIELYIVEPQSRYLFVGNEYSFEVIQSPSDGIVYSSDEGPNQNRKQPMAIKSPSGRVHELVKSDPHFPYGTWKGSIKIKEPGVWSALVIADSGIGWSVFAEWLCV A1B7FD5B7076EC7F A1B7FD5B7076EC7F sho1_yeast High osmolarity signaling protein SHO1 YER118C SHO1 SSU81 Osmosensor SHO1 Suppressor of SUA8-1 mutation Synthetic high osmolarity-sensitive protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSISSKIRPTPRKPSRMATDHSFKMKKFYADPFAISSISLAIVSWVIAIGGSISSASTNESFPRFTWWGIVYQFLIICSLMLFYCFDLVDHYRIFITTSIAVAFVYNTNSATNLVYADGPKKAAASAGVILLSIINLIWILYYGGDNASPTNRWIDSFSIKGIRPSPLENSLHRARRRGNRNTTPYQNNVYNDAIRDSGYATQFDGYPQQQPSHTNYVSSTALAGFENTQPNTSEAVNLHLNTLQQRINSASNAKETNDNSNNQTNTNIGNTFDTDFSNGNTETTMGDTLGLYSDIGDDNFIYKAKALYPYDADDDDAYEISFEQNEILQVSDIEGRWWKARRANGETGIIPSNYVQLIDGPEEMHR E467A4D50AA3EDB6 E467A4D50AA3EDB6 ubc9_yeast SUMO-conjugating enzyme UBC9 UBC9 Ubiquitin-conjugating enzyme E2-18 kDa Ubiquitin-protein ligase Ubiquitin carrier protein 9 YDL064W RING-type E3 SUMO transferase UBC9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSLCLQRLQEERKKWRKDHPFGFYAKPVKKADGSMDLQKWEAGIPGKEGTNWAGGVYPITVEYPNEYPSKPPKVKFPAGFYHPNVYPSGTICLSILNEDQDWRPAITLKQIVLGVQDLLDSPNPNSPAQEPAWRSFSRNKAEYDKKVLLQAKQYSK D74EE9845BB677B4 D74EE9845BB677B4 cyk2_yeast Cytokinesis protein 2 HOF1 CYK2 YMR032W YM9973.05 Homolog of CDC15 protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYSYEACFWDPNDNGVNILLGHISQGIRSCDSMILFFKQRSELEKDYARRLGAITGKLDKDIGTNMDYGKLNETFNVVLSVEKARAQSHSKQSEILFRQIYTDTKAFAANLQARYTTLSGKIERLRMDKFNKKKGCEVLQKKLQDAQIRFRDLQLNENNMIGAKRVEHNKRELLKWESNSQEYKVQLDVLKQEYKASQKFWIHEWAQLSCELQEMENARISFLQSKLQQFATSSMETYILEQTKMDMLTNHLNSFTAADEISTFSKENGTGRLKHKTSKGDMNSSANWAQMSSISTTSKKTESYMDNIRKLSSQLKETENKRKLASIDKYEKPLPSPEVTMATQFRNSTPVIRNETKVVANPTLSLRSSPVQLQSNVDDSVLRQKPDKPRPIVGEEQLKPDEDSKNPDEKGLMVHKRNQSLSSPSESSSSNPTDFSHIKKRQSMESMTTSVSSMANSIDDSQRFAKSWNSSNRKRKSMSHLQVPSSASSRSDDGGRTPNSAHNLNEDDYNTRRDTSTSTILFKPPVAVRGTSRGHTHRQSMIMQDSSNPIEDALYEMERIQSSSKPGTKTGNIMDERGVVRDRGITVTLPIVTSEGFPVIEYAKAMYPLIGNEAPGLANFHKGDYLLITEIVNKDWYKGEVYDNDRIDRNHRIGLIPYNFIQLLHQGL AE4A76C2F4043021 AE4A76C2F4043021 inn1_yeast Ingression protein 1 N1765 YNL152W INN1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEEVWNGNQGILSVYVSKARDLPNLNKLDKQNVMLRLRIAHMTRASNTLHRAGQNPVFHYLEKFDITPEIKPLMYVEVYCDRRKKSPLPIGRCEIDLLNAIRADPKEGYCTWYELKRSGDEFAGTIFIELTFTPKVPRLNRDDLNKEMDRLDSSMAMRPIPPLPTESEYDYVHGSTMRQITPQCVSTSHEDKDEGQPYRNGNVFSMSSKSDTAVLANSNDPIILPPTFSASMGTTSTLETNDTAISNTSNTKFHFANLRKLKEKINIFKNPDSSTNNCQNESNKVDIEALQKAIGVTSLSYDEDDDDDDENDAFYSSSHRVSHNYNQPPLPPIPTRDDMSNYSSSRNTPLVRRDRPSRLDSSSPNSHPHPSGLNSPKLPPLPTTSNSNFNSRKNSMSPTRKRPPPRLS 66C2B81D21CE5520 chs3_yeast Chitin synthase 3 CHS3 CAL1 DIT101 KIT2 YBR023C CSD2 YBR0305 Chitin-UDP acetyl-glucosaminyl transferase 3 Class-IV chitin synthase 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTGLNGDDPDDYYLNLNQDEESLLRSRHSVGSGAPHRQGSLVRPERSRLNNPDNPHFYYAQKTQEQMNHLDVLPSSTGVNPNATRRSGSLRSKGSVRSKFSGRETDSYLLQDMNTTDKKASVKISDEGVAEDEFDKDGDVDNFEESSTQPINKSIKPLRKETNDTLSFWQMYCYFITFWAPAPILAFCGMPKKERQMAWREKVALISVILYIGAIVAFLTFGFTKTVCSSSKLRLKNNEVSTEFVVINGKAYELDTSSRSGIQDVEVDSDTLYGPWSDAGKDASFLFQNVNGNCHNLITPKSNSSIPHDDDNNLAWYFPCKLKNQDGSSKPNFTVENYAGWNCHTSKEDRDAFYGLKSKADVYFTWDGIKNSSRNLIVYNGDVLDLDLLDWLEKDDVDYPVVFDDLKTSNLQGYDLSLVLSNGHERKIARCLSEIIKVGEVDSKTVGCIASDVVLYVSLVFILSVVIIKFIIACYFRWTVARKQGAYIVDNKTMDKHTNDIEDWSNNIQTKAPLKEVDPHLRPKKYSKKSLGHKRASTFDLLKKHSSKMFQFNESVIDLDTSMSSSLQSSGSYRGMTTMTTQNAWKLSNENKAVHSRNPSTLLPTSSMFWNKATSSPVPGSSLIQSLDSTIIHPDIVQQPPLDFMPYGFPLIHTICFVTCYSEDEEGLRTTLDSLSTTDYPNSHKLLMVVCDGLIKGSGNDKTTPEIALGMMDDFVTPPDEVKPYSYVAVASGSKRHNMAKIYAGFYKYDDSTIPPENQQRVPIITIVKCGTPAEQGAAKPGNRGKRDSQIILMSFLEKITFDERMTQLEFQLLKNIWQITGLMADFYETVLMVDADTKVFPDALTHMVAEMVKDPLIMGLCGETKIANKAQSWVTAIQVFEYYISHHQAKAFESVFGSVTCLPGCFSMYRIKSPKGSDGYWVPVLANPDIVERYSDNVTNTLHKKNLLLLGEDRFLSSLMLKTFPKRKQVFVPKAACKTIAPDKFKVLLSQRRRWINSTVHNLFELVLIRDLCGTFCFSMQFVIGIELIGTMVLPLAICFTIYVIIFAIVSKPTPVITLVLLAIILGLPGLIVVITATRWSYLWWMCVYICALPIWNFVLPSYAYWKFDDFSWGDTRTIAGGNKKAQDENEGEFDHSKIKMRTWREFEREDILNRKEESDSFVA EBF9227DC30D3EA4 msb1_yeast Morphogenesis-related protein MSB1 MSB1 Multicopy suppressor of bud emergence 1 YOR188W protein protein yeast Saccharomyces cerevisiae Baker's yeast MNDMAKPLPTPPTAEIRKSRSNSPKKAQKTNLSPNKNQNNEKNVPRSNGRTKNEHNSMDDEEFEFFHQFSREKVKGVIHVITAELKEKGPDVEFLMIPFRPEQTNDKLLTLLNQLFPLGNGQPVNEKKQLRIVSKADVWTLFQCLKYIWCRLPNSEIIGWKSYLEFKFREEDKKFPRKSFLEIMPQCLASPNHASIVYDFFDLIISISSNSRVNKMSARKISKMCAIWAFSKQIPNSDIQDYDFESAAMKSFAPNNSIQDGLDQWIPASDAMFHLLLAFLRSFVPQDLESAKLPRTLKSLLFNNQYPPRKSTAYTSETILTIPLVTLKTDVFSRKPWQLLERCNDLLDFSDHDAFEAREDYALLKSLFRKKNTVEGISRKMSQESRRLMKAMSTKHSTFQPGWAPRECIENISHLKECIEVKRLDIDDYFIWTWLSSLSFEQTSEKKKIFGRSIILEFEFDGFKKWVVFQECDITLDYNKKGQLKKKTSAQSPTTEKELPPDDFELEDPPLSKSPTLSQTYKKFQAEVPQQSTVRRDSAPDNQGIYHTVISKNALTKNKHNVNLHSFEHKISKWNPLNNLRKKSGSNSSSSSFEEKSKDAPIREEYHTNKNHKSKKEERVLSQFSTLNPDEYQLPVIETGSSNFKIEIPELMYEHDDDDSDKLKNSQKRATDSAIEELNGMVEEMMINEPDDVKISITEAETFESLTKFDQYKPSNITDDDLQSSHSSAVHSLKLSTNTNDSCADSSKYTADRKLAEPRKISEESKVNDDSSSYYSPNINNLPASRMPSQPTYSNSDSKKAFTNESRLNVLQGAVSPSQQVTPKPYKNAPGDCVSPVQQKYYQNDRRNEMSPASAPVPPSAYSPARSPQFSTNSAGFKQNTINVPVGYNDPAHVLANQPHMTYRDQHNYPSHQQKQRPFQNNIVPPELKSRNQRADASPIPQHMVPVKQGVPNLPSNVPLYQQMERMNPNHQHPVNTYKVTQPPYHNNTTNAYGNSRAGNAHMLDGKWSNNPPQMVPKGVRPNQYPQQHVNRYSPQAQPVVPAEYYNGPPPMRAPPMMSHMVPAQEPIRYTAGANRRSFPQGMQQNAYSVPAQPMGAVNSEFYLPEAPQGNKLHGNINKRQERKKLYDNIRSGNFGI 59CDD6E0F1E43F8A sro7_yeast Lethal(2) giant larvae protein homolog SRO7 SRO7 SNI1 YPR032W YP9367.12 SOP1 Suppressor of RHO3 protein 7 Polarity protein SRO7 Sodium protection protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFGSKRLKNVKEAFKSLKGQNSETPIENSKASFKSKNSKTSTISKDAKSSSSLKIPISSNNKNKIFSLAETNKYGMSSKPIAAAFDFTQNLLAIATVTGEVHIYGQQQVEVVIKLEDRSAIKEMRFVKGIYLVVINAKDTVYVLSLYSQKVLTTVFVPGKITSIDTDASLDWMLIGLQNGSMIVYDIDRDQLSSFKLDNLQKSSFFPAARLSPIVSIQWNPRDIGTVLISYEYVTLTYSLVENEIKQSFIYELPPFAPGGDFSEKTNEKRTPKVIQSLYHPNSLHIITIHEDNSLVFWDANSGHMIMARTVFETEINVPQPDYIRDSSTNAAKISKVYWMCENNPEYTSLLISHKSISRGDNQSLTMIDLGYTPRYSITSYEGMKNYYANPKQMKIFPLPTNVPIVNILPIPRQSPYFAGCHNPGLILLILGNGEIETMLYPSGIFTDKASLFPQNLSWLRPLATTSMAASVPNKLWLGALSAAQNKDYLLKGGVRTKRQKLPAEYGTAFITGHSNGSVRIYDASHGDIQDNASFEVNLSRTLNKAKELAVDKISFAAETLELAVSIETGDVVLFKYEVNQFYSVENRPESGDLEMNFRRFSLNNTNGVLVDVRDRAPTGVRQGFMPSTAVHANKGKTSAINNSNIGFVGIAYAAGSLMLIDRRGPAIIYMENIREISGAQSACVTCIEFVIMEYGDDGYSSILMVCGTDMGEVITYKILPASGGKFDVQLMDITNVTSKGPIHKIDAFSKETKSSCLATIPKMQNLSKGLCIPGIVLITGFDDIRLITLGKSKSTHKGFKYPLAATGLSYISTVEKNNDRKNLTVIITLEINGHLRVFTIPDFKEQMSEHIPFPIAAKYITESSVLRNGDIAIRVSEFQASLFSTVKEQDTLAPVSDTLYINGIRIPYRPQVNSLQWARGTVYCTPAQLNELLGGVNRPASKYKESIIAEGSFSERSSDDNNANHPEHQYTKPTRKGRNSSYGVLRNVSRAVETRWDAVEDRFNDYATAMGETMNEAVEQTGKDVMKGALGF FFB0901883009815 FFB0901883009815 sho1-sro7-1 2 18 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, Supp Table 1 sho1-chs3-1 2 16 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, Supp Table 1 sho1-cyk3-1 2 11 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, Supp Table 1 sho1-hof1-1 2 14 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, Supp Table 1 sho1-sho1-1 2 12 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, Supp Table 1 sho1-msb1-1 2 17 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, Supp Table 1 sho1-inn1-1 2 15 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, Supp Table 1 sho1-pbs2-1 2 5 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, 4A, Supp Table 1 hof1-inn1-1 2 15 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 hof1-cdc3-1 2 8 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 hof1-cyk3-1 2 11 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 hof1-nba1-2 2 6 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 14 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 inn1-hof1-1 2 14 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 inn1-cdc14-1 2 9 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 15 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 inn1-ubc9-1 2 13 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 15 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 inn1-ubc9-2 2 10 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 15 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 cyk3-hof1-1 2 14 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 cyk3-tpk1-1 2 7 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 cyk3-bud14-1 2 3 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 cyk3-nba1-2 2 6 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 cyk3-inn1-1 2 15 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 cyk3-slt2-1 2 4 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role bait bait region cub cub undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Table 1 sho1-pbs2-2 1 5 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 1B sho1-hof1-2 1 14 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 12 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig. 1B sho1-cyk3-2 1 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 1B sho1-inn1-2 1 15 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeast Saccharomyces cerevisiae Baker's yeast 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association Fig. 1B sho1-cyk3-3 1 11 unspecified role unspecified role prey prey agt tagged region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain PPLPPLP ASLASLA ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 172 169 physical association physical association Fig. 1C AGT–Cyk3 aas 160–210 directly interacted with the purified GST fusion of SH3Sho1 (Fig. 1C). sho1-hof1-3 1 14 unspecified role unspecified role prey prey n-terminal nub nub n-term range n-terminal range n-term range n-term range n-terminal range n-term range region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 12 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Supp. Fig. 1B sho1-hof1-4 1 14 unspecified role unspecified role prey prey agt tagged region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 188 185 physical association physical association Fig. 1D Pull-down assays with bacterially expressed Hof1p fragments showed a specific and direct interaction between the purified SH3Sho1 and Hof1269–349, harboring P1. sho1-inn1-3 1 15 unspecified role unspecified role prey prey agt tagged region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 194 192 physical association physical association Fig. 1E sho1-msb1-2 1 17 unspecified role unspecified role prey prey agt tagged region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 199 201 physical association physical association Fig. 1F hof1-cyk3-2 1 11 unspecified role unspecified role prey prey agt tagged region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role bait bait n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2D