DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The structural basis of transferrin sequestration by transferrin-binding protein B. The structural basis of transferrin sequestration by transferrin-binding protein B. Nature structural & molecular biology 2012 imex curation Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. DIP original experiment: DIP-250832X Nature structural & molecular biology 2012 IMEx The structural basis of transferrin sequestration by transferrin-binding protein B. The structural basis of transferrin sequestration by transferrin-binding protein B. The structural basis of transferrin sequestration by transferrin-binding protein B. Nature structural & molecular biology 2012 imex curation Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. DIP original experiment: DIP-250833X Nature structural & molecular biology 2012 IMEx The structural basis of transferrin sequestration by transferrin-binding protein B. The structural basis of transferrin sequestration by transferrin-binding protein B. The structural basis of transferrin sequestration by transferrin-binding protein B. Nature structural & molecular biology 2012 imex curation Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Calmettes C., Alcantara J., Yu RH., Schryvers AB., Moraes TF. DIP original experiment: DIP-250831X Nature structural & molecular biology 2012 IMEx The structural basis of transferrin sequestration by transferrin-binding protein B. trfe_human Serotransferrin Siderophilin Beta-1 metal-binding globulin TF PRO1400 protein protein human Homo sapiens Human MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP DIP protein P02787 original sequence version: 123 DIP protein P02787 original sequence version: 144 DIP protein P02787 original sequence version: 156 DIP protein P02787 original sequence version: 165 DIP protein P02787 original sequence version: 161 DIP protein P02787 original sequence version: 170 9A73B90D8C5671E9 tbpb1_neimi Transferrin-binding protein B tbpB tbp2 TbpB receptor Transferrin-binding protein 2 protein protein Neisseria meningitidis serogroup B Neisseria meningitidis serogroup B MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKYQDVSSEKPQAQKDQGGYGFAMRLKRRNWYPGAEESEVKLNESDWEATGLPTKPKELPKRQKSVIEKVETDGDSDIYSSPYLTPSNHQNGSAGNGVNQPKNQATGHENFQYVYSGWFYKHAASEKDFSNKKIKSGDDGYIFYHGEKPSRQLPASGKVIYKGVWHFVTDTKKGQDFREIIQPSKKQGDRYSGFSGDGSEEYSNKNESTLKDDHEGYGFTSNLEVDFGNKKLTGKLIRNNASLNNNTNNDKHTTQYYSLDAQITGNRFNGTATATDKKENETKLHPFVSDSSSLSGGFFGPQGEELGFRFLSDDQKVAVVGSAKTKDKLENGAAASGSTGAAASGGAAGTSSENSKLTTVLDAVELTLNDKKIKNLDNFSNAAQLVVDGIMIPLLPKDSESGNTQADKGKNGGTEFTRKFEHTPESDKKDAQAGTQTNGAQTASNTAGDTNGKTKTYEVEVCCSNLNYLKYGMLTRKNSKSAMQAGGNSSQADAKTEQVEQSMFLQGERTDEKEIPTDQNVVYRGSWYGHIANGTSWSGNASDKEGGNRAEFTVNFADKKITGKLTAENRQAQTFTIEGMIQGNGFEGTAKTAESGFDLDQKNTTRTPKAYITDAKVKGGFYGPKAEELGGWFAYPGDKQTEKATATSSDGNSASSATVVFGAKRQQPVQ DIP protein Q09057 original sequence version: 59 tbpb-tf-3 3 4 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human 5 unspecified role unspecified role unspecified role unspecified role Region 56-711 Region 56-711 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tbpb-tf-4 1 4 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human 5 unspecified role unspecified role unspecified role unspecified role Region 56-711 Region 56-711 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction tbpb-tf-5 2 4 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait Region 56-711 Region 56-711 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction