DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Structure (London, England : 1993) 2012 imex curation Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. DIP original experiment: DIP-249261X Structure (London, England : 1993) 2012 IMEx Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Structure (London, England : 1993) 2012 imex curation Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. Only protein-protein interactions ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. DIP original experiment: DIP-249262X Structure (London, England : 1993) 2012 IMEx Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Structure (London, England : 1993) 2012 imex curation Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. DIP original experiment: DIP-249260X Structure (London, England : 1993) 2012 IMEx Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. Structure (London, England : 1993) 2012 imex curation Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Feldman-Salit A., Wirtz M., Lenherr ED., Throm C., Hothorn M., Scheffzek K., Hell R., Wade RC. DIP original experiment: DIP-249263X Structure (London, England : 1993) 2012 IMEx Allosterically gated enzyme dynamics in the cysteine synthase complex regulate cysteine biosynthesis in Arabidopsis thaliana. sat3_arath Serine acetyltransferase 3, mitochondrial SAT3 SAT1 SATA MJG19.6 At3g13110 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLPVTSRRHFTMSLYMLRSSSPHINHHSFLLPSFVSSKFKHHTLSPPPSPPPPPPMAACIDTCRTGKPQISPRDSSKHHDDESGFRYMNYFRYPDRSSFNGTQTKTLHTRPLLEDLDRDAEVDDVWAKIREEAKSDIAKEPIVSAYYHASIVSQRSLEAALANTLSVKLSNLNLPSNTLFDLFSGVLQGNPDIVESVKLDLLAVKERDPACISYVHCFLHFKGFLACQAHRIAHELWTQDRKILALLIQNRVSEAFAVDFHPGAKIGTGILLDHATAIVIGETAVVGNNVSILHNVTLGGTGKQCGDRHPKIGDGVLIGAGTCILGNITIGEGAKIGAGSVVLKDVPPRTTAVGNPARLLGGKDNPKTHDKIPGLTMDQTSHISEWSDYVI DIP protein Q39218 original sequence version: 81 A3ED09DEA53C10E2 cyskm_arath Cysteine synthase, mitochondrial Beta-substituted Ala synthase 2;2 OASC ACS 1 F24G16.30 At3g59760 O-acetylserine sulfhydrylase CSase C O-acetylserine (thiol)-lyase OAS-TL C protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVAMIMASRFNREAKLASQILSTLLGNRSCYTSMAATSSSALLLNPLTSSSSSSTLRRFRCSPEISSLSFSSASDFSLAMKRQSRSFADGSERDPSVVCEAVKRETGPDGLNIADNVSQLIGKTPMVYLNSIAKGCVANIAAKLEIMEPCCSVKDRIGYSMVTDAEQKGFISPGKSVLVEPTSGNTGIGLAFIAASRGYRLILTMPASMSMERRVLLKAFGAELVLTDPAKGMTGAVQKAEEILKNTPDAYMLQQFDNPANPKIHYETTGPEIWDDTKGKVDIFVAGIGTGGTITGVGRFIKEKNPKTQVIGVEPTESDILSGGKPGPHKIQGIGAGFIPKNLDQKIMDEVIAISSEEAIETAKQLALKEGLMVGISSGAAAAAAIKVAKRPENAGKLIAVVFPSFGERYLSTPLFQSIREEVEKMQPEV DIP protein Q43725 original sequence version: 102 9DDEB1B8E314E143 oasc-sat3-1 4 5 unspecified role unspecified role unspecified role unspecified role Region 355-391 Region 355-391 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction oasc-1 3 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction oasc-2 1 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction sat3-oasc-3 2 5 unspecified role unspecified role bait bait Region 387-391 Region 387-391 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction