DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Nature structural & molecular biology 2012 imex curation Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. DIP original experiment: DIP-250799X Nature structural & molecular biology 2012 IMEx Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Nature structural & molecular biology 2012 imex curation Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. DIP original experiment: DIP-250797X Nature structural & molecular biology 2012 IMEx Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Nature structural & molecular biology 2012 imex curation Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. DIP original experiment: DIP-250800X Nature structural & molecular biology 2012 IMEx Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Nature structural & molecular biology 2012 imex curation Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. DIP original experiment: DIP-250802X Nature structural & molecular biology 2012 IMEx Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Nature structural & molecular biology 2012 imex curation Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. DIP original experiment: DIP-250798X Nature structural & molecular biology 2012 IMEx Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Nature structural & molecular biology 2012 imex curation Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. DIP original experiment: DIP-250803X Nature structural & molecular biology 2012 IMEx Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Structure and mechanism of the UvrA-UvrB DNA damage sensor. Nature structural & molecular biology 2012 imex curation Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Pakotiprapha D., Samuels M., Shen K., Hu JH., Jeruzalmi D. DIP original experiment: DIP-250801X Nature structural & molecular biology 2012 IMEx Structure and mechanism of the UvrA-UvrB DNA damage sensor. a0a0e0tgm2_geos2 UvrABC system protein B uvrB Excinuclease ABC subunit B GYMC52_3204 protein protein geos2 Geobacillus sp. (strain Y412MC52) MEGRFQLVSPYEPQGDQPQAIAKLVDGLRLGVKHQTLLGATGTGKTFTISNVIAQVNKPTLVIAHNKTLAGQLYSELKEFFPHNAVEYFVSYYDYYQPEAYVPQTDTYIEKDAKINDEIDKLRHSATSALFERRDVIIVASVSCIYGLGSPEEYRELVVSLRVGMEIERNALLRRLVDIQYDRNDIDFRRGTFRVRGDVVEIFPASRDEHCIRVEFFGDEIERIREVDALTGEVLGEREHVAIFPASHFVTREEKMRLAIQNIEQELEERLAELRAQGKLLEAQRLEQRTRYDLEMMREMGFCSGIENYSRHLALRPPGSTPYTLLDYFPDDFLIIVDESHVTLPQLRGMYNGDRARKQVLVDHGFRLPSALDNRPLTFEEFEQKINQIIYISATPGPYELEHSPGVVEQIIRPTGLLDPTIDVRPTKGQIDDLIGEIHERVERNERTLVTTLTKKMAEDLTDYLKEAGIKVAYLHSEIKTLERIEIIRDLRLGKYDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARNANGHVIMYADTITKSMEIAIQETKRRRAIQEEYNRKHGIVPRTVKKEIRDVIRATYAAEETEMYEAKPAAAMTKQEREELIRKLEAEMKEAAKALDFERAAQLRDIIFELKAEG a0a0e0tg05_geos2 UvrABC system protein A uvrA Excinuclease ABC subunit A GYMC52_3203 protein protein geos2 Geobacillus sp. (strain Y412MC52) MNGMDKIIVKGARAHNLKNIDVEIPRGKLVVLTGLSGSGKSSLAFDTIYAEGQRRYVESLSAYARQFLGQMEKPDVDAIEGLSPAISIDQKTTSRNPRSTVGTVTEIYDYLRLLFARIGRPICPTHGIEIQSQTIEQMVDRLLSYPERTKMQILAPIVSGKKGTHAKTLEDIRKQGYVRVRIDREMRELTGDIELEKNKKHSIDVVVDRIIIKDGIAARLADSLETALKLADGKVVVDVIGEGELLFSEKHACPYCGFSIGELEPRLFSFNSPFGACPDCDGLGAKLEVDLDLVIPNDELTLKEHAIAPWEPQSSQYYPQLLEAVCRHYGIPMDVPVKDLPKEQLDKILYGSGGEPIYFRYTNDFGQVREQYIAFEGVIPNVERRYRETSSDYIREQMEKYMAEQPCPTCQGYRLKKESLAVLVGGKHIGEVTAMSVTEALAFFDGLELTEKEAQIARLILREIRDRLGFLQNVGLDYLTLSRSAGTLSGGEAQRIRLATQIGSRLTGVLYVLDEPSIGLHQRDNDRLIATLKSMRDLGNTLIVVEHDEDTMLAADYLIDIGPGAGIHGGEVVAAGTPEEVMNDPNSLTGQYLSGKKFIPIPAERRRPDGRWLEVVGAREHNLKNVSVKIPLGTFVAVTGVSGSGKSTLVNEVLYKALAQKLHRAKAKPGEHRDIRGLEHLDKVIDIDQSPIGRTPRSNPATYTGVFDDIRDVFASTNEAKVRGYKKGRFSFNVKGGRCEACHGDGIIKIEMHFLPDVYVPCEVCHGKRYNRETLEVTYKGKNIAEVLDMTVEDALDFFASIPKIKRKLETLYDVGLGYMKLGQPATTLSGGEAQRVKLAAELHRRSNGRTLYILDEPTTGLHVDDIARLLDVLHRLVDNGDTVLVIEHNLDVIKTADYIIDLGPEGGDRGGQIVAVGTPEEVAEVKESHTGRYLKPILERDRARMQARYEAAKA uvra-uvrb-4 2 8 unspecified role unspecified role unspecified role unspecified role A0A0E0TGM2:p.[Glu295Arg;Glu299Arg] E295R/E299R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E R certain certain sequence position certain certain certain sequence position certain E R Fri Jul 28 10:42:50 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'E295arg,glu299arg' Wed May 02 16:13:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu295arg,glu299arg' Tue Jun 30 19:37:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu295Arg;Glu299Arg]' ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction uvra-4 4 9 unspecified role unspecified role unspecified role unspecified role Region 4-120/423-955 Region 4-120/423-955 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction uvra-5 6 9 unspecified role unspecified role unspecified role unspecified role Region 4-120/423-955 Region 4-120/423-955 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction uvra-uvrb-5 3 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction uvra-uvrb-6 5 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction uvra-uvrb-7 7 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role A0A0E0TG05:p.Lys40Ala K40A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A Wed May 02 16:52:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys40ala' Tue Jun 30 16:33:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys40Ala' ecolx Escherichia coli direct interaction direct interaction uvra-uvrb-8 1 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction