IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation. The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation. Genes & development 2012 imex curation Lavy T., Kumar PR., He H., Joshua-Tor L. Gal3 dimer crystal not curated as the authors state this is a crystalllization artefact and the protein is monomeric. leemor@cshl.edu Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lavy T., Kumar PR., He H., Joshua-Tor L. Gal3 dimer crystal not curated as the authors state this is a crystalllization artefact and the protein is monomeric. leemor@cshl.edu Accepted 2017-FEB-27 AT 10:28 GMT AT 10:28 GMT by MGT Genes & development 2012 imex curation alpha-d-galactose alpha-D-galactose ALPHA D-GALACTOSE alpha-D-Gal alpha-D-Galactose alpha-D-galactose Gal-alpha alpha-D-galactopyranose small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) mgatp(2-) MgATP(2-) [MgATP](2-) Mg(atp)(2-) (adenosine 5'-triphosphato)magnesium(2-) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) gal3_yeast Protein GAL3 YD8119.14 YDR009W GAL3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNTNVPIFSSPVRDLPRSFEQKHLAVVDAFFQTYHVKPDFIARSPGRVNLIGEHIDYCDFSVLPLAIDVDMLCAVKILDEKNPSITLTNADPKFAQRKFDLPLDGSYMAIDPSVSEWSNYFKCGLHVAHSYLKKIAPERFNNTPLVGAQIFCQSDIPTGGGLSSAFTCAAALATIRANMGKNFDISKKDLTRITAVAEHYVGVNNGGMDQATSVYGEEDHALYVEFRPKLKATPFKFPQLKNHEISFVIANTLVKSNKFETAPTNYNLRVIEVTVAANALATRYSVALPSHKDNSNSERGNLRDFMDAYYARYENQAQPWNGDIGTGIERLLKMLQLVEESFSRKKSGFTVHEASTALNCSREEFTRDYLTTFPVRFQVLKLYQRAKHVYSESLRVLKALKMMTSATFHTDEDFFTDFGRLMNESQASCDKLYECSCIETNQICSIALANGSFGSRLTGAGWGGCTIHLVPSGANGNVEQVRKALIEKFYNVRYPDLTDEELKDAIIVSKPALGTCLYEQ 5852028EBDDC0F3B 5852028EBDDC0F3B gal80_yeast Galactose/lactose metabolism regulatory protein GAL80 GAL80 YM9958.12 YM9827.01 YML051W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLSSQFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEVVMPLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILRAKELISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDILQYMTSSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVSCSFKGGKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANGQQAPLDPGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQGFDFEGFPTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL 1492473684F3894C 1492473684F3894C alpha_d_galactose-gal3-1 1 4 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role unspecified role unspecified role 2 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2 2.1 A R work - 0.15 R free- 0.196