DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Science (New York, N.Y.) 2012 imex curation Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. DIP original experiment: DIP-268293X Science (New York, N.Y.) 2012 IMEx Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Science (New York, N.Y.) 2012 imex curation Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. DIP original experiment: DIP-268288X Science (New York, N.Y.) 2012 IMEx Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Science (New York, N.Y.) 2012 imex curation Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. DIP original experiment: DIP-268290X Science (New York, N.Y.) 2012 IMEx Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Science (New York, N.Y.) 2012 imex curation Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. DIP original experiment: DIP-268289X Science (New York, N.Y.) 2012 IMEx Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Science (New York, N.Y.) 2012 imex curation Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. DIP original experiment: DIP-268291X Science (New York, N.Y.) 2012 IMEx Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. Science (New York, N.Y.) 2012 imex curation Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Adrain C., Zettl M., Christova Y., Taylor N., Freeman M. DIP original experiment: DIP-268292X Science (New York, N.Y.) 2012 IMEx Tumor necrosis factor signaling requires iRhom2 to promote trafficking and activation of TACE. ada17_mouse Disintegrin and metalloproteinase domain-containing protein 17 Adam17 Tace TNF-alpha-converting enzyme TNF-alpha convertase protein protein mouse Mus musculus Mouse MRRRLLILTTLVPFVLAPRPPEEAGSGSHPRLEKLDSLLSDYDILSLANIQQHSIRKRDLQSATHLETLLTFSALKRHFKLYLTSSTERFSQNLRVVVVDGKEESEYSVKWQNFFSGHVVGEPDSRVLAHIGDDDVTVRINTDGAEYNVEPLWRFVNDTKDKRMLVYKSEDIKDFSRLQSPKVCGYLNADSEELLPKGLIDREPSEEFVRRVKRRAEPNPLKNTCKLLVVADHRFYKYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGVQIEQIRILKSPQEVKPGERHFNMAKSFPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYNPTVKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKPGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGDAEDDTVCLDLGKCKAGKCIPFCKREQELESCACVDTDNSCKVCCRNLSGPCVPYVDAEQKNLFLRKGKPCTVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDKQYESLSLFHHSNIEMLSSMDSASVRIIKPFPAPQTPGRLQALQPAAMMPPVPAAPKLDHQRMDTIQEDPSTDSHADDDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQSRVDSKETEC DIP protein Q9Z0F8 original sequence version: 157 Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway mint homomint 6B434F80878197F5 rhdf2_mouse Inactive rhomboid protein 2 Rhor Rhomboid veinlet-like protein 6 Rhomboid-related protein Rhbdf2 Rhbdl6 Irhom2 Rhomboid family member 2 protein protein mouse Mus musculus Mouse MASADKNGSNLPSVSGSRLQSRKPPNLSITIPPPESQAPGEQDSMLPERRKNPAYLKSVSLQEPRGRWQEGAEKRPGFRRQASLSQSIRKSTAQWFGVSGDWEGKRQNWHRRSLHHCSVHYGRLKASCQRELELPSQEVPSFQGTESPKPCKMPKIVDPLARGRAFRHPDEVDRPHAAHPPLTPGVLSLTSFTSVRSGYSHLPRRKRISVAHMSFQAAAALLKGRSVLDATGQRCRHVKRSFAYPSFLEEDAVDGADTFDSSFFSKEEMSSMPDDVFESPPLSASYFRGVPHSASPVSPDGVHIPLKEYSGGRALGPGTQRGKRIASKVKHFAFDRKKRHYGLGVVGNWLNRSYRRSISSTVQRQLESFDSHRPYFTYWLTFVHIIITLLVICTYGIAPVGFAQHVTTQLVLKNRGVYESVKYIQQENFWIGPSSIDLIHLGAKFSPCIRKDQQIEQLVRRERDIERTSGCCVQNDRSGCIQTLKKDCSETLATFVKWQNDTGPSDKSDLSQKQPSAVVCHQDPRTCEEPASSGAHIWPDDITKWPICTEQAQSNHTGLLHIDCKIKGRPCCIGTKGSCEITTREYCEFMHGYFHEDATLCSQVHCLDKVCGLLPFLNPEVPDQFYRIWLSLFLHAGIVHCLVSVVFQMTILRDLEKLAGWHRISIIFILSGITGNLASAIFLPYRAEVGPAGSQFGLLACLFVELFQSWQLLERPWKAFFNLSAIVLFLFICGLLPWIDNIAHIFGFLSGMLLAFAFLPYITFGTSDKYRKRALILVSLLVFAGLFASLVLWLYIYPINWPWIEYLTCFPFTSRFCEKYELDQVLH DIP protein Q80WQ6 original sequence version: 89 650052E5A4BD9CA5 ada17_human Disintegrin and metalloproteinase domain-containing protein 17 ADAM17 CSVP TACE TNF-alpha-converting enzyme TNF-alpha convertase Snake venom-like protease protein protein human Homo sapiens Human MRQSLLFLTSVVPFVLAPRPPDDPGFGPHQRLEKLDSLLSDYDILSLSNIQQHSVRKRDLQTSTHVETLLTFSALKRHFKLYLTSSTERFSQNFKVVVVDGKNESEYTVKWQDFFTGHVVGEPDSRVLAHIRDDDVIIRINTDGAEYNIEPLWRFVNDTKDKRMLVYKSEDIKNVSRLQSPKVCGYLKVDNEELLPKGLVDREPPEELVHRVKRRADPDPMKNTCKLLVVADHRFYRYMGRGEESTTTNYLIELIDRVDDIYRNTSWDNAGFKGYGIQIEQIRILKSPQEVKPGEKHYNMAKSYPNEEKDAWDVKMLLEQFSFDIAEEASKVCLAHLFTYQDFDMGTLGLAYVGSPRANSHGGVCPKAYYSPVGKKNIYLNSGLTSTKNYGKTILTKEADLVTTHELGHNFGAEHDPDGLAECAPNEDQGGKYVMYPIAVSGDHENNKMFSNCSKQSIYKTIESKAQECFQERSNKVCGNSRVDEGEECDPGIMYLNNDTCCNSDCTLKEGVQCSDRNSPCCKNCQFETAQKKCQEAINATCKGVSYCTGNSSECPPPGNAEDDTVCLDLGKCKDGKCIPFCEREQQLESCACNETDNSCKVCCRDLSGRCVPYVDAEQKNLFLRKGKPCTVGFCDMNGKCEKRVQDVIERFWDFIDQLSINTFGKFLADNIVGSVLVFSLIFWIPFSILVHCVDKKLDKQYESLSLFHPSNVEMLSSMDSASVRIIKPFPAPQTPGRLQPAPVIPSAPAAPKLDHQRMDTIQEDPSTDSHMDEDGFEKDPFPNSSTAAKSFEDLTDHPVTRSEKAASFKLQRQNRVDSKETEC DIP protein P78536 original sequence version: 170 DIP protein P78536 original sequence version: 184 5B1032F6B88A837F rhbdf2-adam17-1 2 7 unspecified role unspecified role prey prey V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association rhbdf2-adam17-2 5 9 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association adam17-rhbdf2-1 1 9 unspecified role unspecified role bait bait human Homo sapiens Human 8 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association rhbdf2-adam17-3 4 7 unspecified role unspecified role prey prey mouse Mus musculus Mouse 8 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association rhbdf2-adam17-4 6 7 unspecified role unspecified role prey prey Strep-tag Strep-tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human Homo sapiens Human physical association physical association adam17-rhbdf2-2 3 7 unspecified role unspecified role bait bait mouse Mus musculus Mouse 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association