DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. DIP original experiment: DIP-253534X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. DIP original experiment: DIP-253538X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. DIP original experiment: DIP-253539X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. DIP original experiment: DIP-253537X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. DIP original experiment: DIP-253533X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. DIP original experiment: DIP-253536X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. DIP original experiment: DIP-253535X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Structural basis of coactivation of liver receptor homolog-1 by β-catenin. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Yumoto F., Nguyen P., Sablin EP., Baxter JD., Webb P., Fletterick RJ. DIP original experiment: DIP-253540X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis of coactivation of liver receptor homolog-1 by β-catenin. andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ DIP protein P10275 original sequence version: 220 DIP protein P10275-1 original sequence version: 213 DIP protein P10275 original sequence version: 216 DIP protein P10275 original sequence version: 143 DIP protein P10275 original sequence version: 174 DIP protein P10275 original sequence version: 169 DIP protein P10275 original sequence version: 188 DIP protein P10275-1 original sequence version: 188 DIP protein P10275 original sequence version: 196 DIP protein P10275 original sequence version: 217 DIP protein P10275-1 original sequence version: 217 DIP protein P10275-1 original sequence version: 207 A73432C55D39AE06 ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E o00482-1 Nuclear receptor subfamily 5 group A member 2 NR5A2 B1F CPF FTF Alpha-1-fetoprotein transcription factor B1-binding factor CYP7A promoter-binding factor Hepatocytic transcription factor Liver receptor homolog 1 B1F2 protein protein human Homo sapiens Human MSSNSDTGDLQESLKHGLTPIGAGLPDRHGSPIPARGRLVMLPKVETEALGLARSHGEQGQMPENMQVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKRYTCIENQNCQIDKTQRKRCPYCRFQKCLSVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRANGLKLEAMSQVIQAMPSDLTISSAIQNIHSASKGLPLNHAALPPTDYDRSPFVTSPISMTMPPHGSLQGYQTYGHFPSRAIKSEYPDPYTSSPESIMGYSYMDSYQTSSPASIPHLILELLKCEPDEPQVQAKIMAYLQQEQANRSKHEKLSTFGLMCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVVHGKEGSIFLVTGQQVDYSIIASQAGATLNNLMSHAQELVAKLRSLQFDQREFVCLKFLVLFSLDVKNLENFQLVEGVQEQVNAALLDYTMCNYPQQTEKFGQLLLRLPEIRAISMQAEEYLYYKHLNGDVPYNNLLIEMLHAKRA DIP protein O00482-1 original sequence version: 134 DIP protein O00482-1 original sequence version: 120 ctnnb1-ar-2 8 9 unspecified role unspecified role prey prey Region 646-919 Region 646-919 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 138-663 Region 138-663 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ctnnb1-nr5a2-1 5 11 unspecified role unspecified role prey prey Region 191-541 Region 191-541 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role bait bait Region 138-663 Region 138-663 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association ctnnb1-nr5a2-2 7 11 unspecified role unspecified role unspecified role unspecified role Region 191-541 Region 191-541 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 138-663 Region 138-663 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ar-ctnnb1-2 3 9 unspecified role unspecified role bait bait Region 646-919 Region 646-919 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role prey prey P35222:p.[Tyr306Ala;Lys345Ala;Trp383Ala] Y306A/K345A/W383A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y306A/K345A/W383A'. Wed May 02 15:08:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr306ala,lys345ala,trp383ala' Tue Jun 30 19:57:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr306Ala;Lys345Ala;Trp383Ala]' P35222:p.Tyr306Ala Y306A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y306A'. Wed May 02 18:39:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr306ala' Tue Jun 30 19:50:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr306Ala' P35222:p.Trp383Ala W383A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W383A'. Wed May 02 16:40:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp383ala' Tue Jun 30 18:55:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp383Ala' P35222:p.Lys345Ala K345A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K345A'. Wed May 02 17:41:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys345ala' Tue Jun 30 19:03:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys345Ala' 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction ctnnb1-nr5a2-3 4 11 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O00482-1:p.[Asp483Ala;Met486Ala;Thr493Ala] D483A/M486A/T493A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain M A certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D483A/M486A/T493A'. Wed May 02 14:39:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp483ala,met486ala,thr493ala' Tue Jun 30 16:02:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp483Ala;Met486Ala;Thr493Ala]' in vitro In vitro 10 unspecified role unspecified role bait bait Region 138-663 Region 138-663 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction ctnnb1-nr5a2-4 1 11 unspecified role unspecified role unspecified role unspecified role Region 191-541 Region 191-541 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 138-663 Region 138-663 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nr5a2-ctnnb1-1 6 11 unspecified role unspecified role bait bait Region 191-541 Region 191-541 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 10 unspecified role unspecified role prey prey P35222:p.[Tyr306Ala;Lys345Ala;Trp383Ala] Y306A/K345A/W383A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y306A/K345A/W383A'. Wed May 02 20:18:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr306ala,lys345ala,trp383ala' Tue Jun 30 19:01:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr306Ala;Lys345Ala;Trp383Ala]' 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction ctnnb1-ar-3 2 9 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role bait bait Region 138-663 Region 138-663 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction