DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Ravilious GE., Nguyen A., Francois JA., Jez JM. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ravilious GE., Nguyen A., Francois JA., Jez JM. DIP original experiment: DIP-251453X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Ravilious GE., Nguyen A., Francois JA., Jez JM. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ravilious GE., Nguyen A., Francois JA., Jez JM. DIP original experiment: DIP-251455X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. Proceedings of the National Academy of Sciences of the United States of America 2012 imex curation Ravilious GE., Nguyen A., Francois JA., Jez JM. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Ravilious GE., Nguyen A., Francois JA., Jez JM. DIP original experiment: DIP-251454X Proceedings of the National Academy of Sciences of the United States of America 2012 IMEx Structural basis and evolution of redox regulation in plant adenosine-5'-phosphosulfate kinase. apk1_arath Adenylyl-sulfate kinase 1, chloroplastic APK1 AKN1 ATP adenosine-5'-phosphosulfate 3'-phosphotransferase 1 Adenosine-5'-phosphosulfate kinase 1 F26C24.11 T6B13.1 At2g14750 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MIAAGAKSLLGLSMASPKGIFDSNSMSNSRSVVVVRACVSMDGSQTLSHNKNGSIPEVKSINGHTGQKQGPLSTVGNSTNIKWHECSVEKVDRQRLLDQKGCVIWVTGLSGSGKSTLACALNQMLYQKGKLCYILDGDNVRHGLNRDLSFKAEDRAENIRRVGEVAKLFADAGIICIASLISPYRTDRDACRSLLPEGDFVEVFMDVPLSVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPLNCEISLGREGGTSPIEMAEKVVGYLDNKGYLQA DIP protein Q43295 original sequence version: 105 CB698643AA09D811 apk1-1 1 4 unspecified role unspecified role unspecified role unspecified role Region 78-276 Region 78-276 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction apk1-2 3 4 unspecified role unspecified role unspecified role unspecified role Region 78-276 Region 78-276 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q43295:p.[Cys86Ala;Cys119Ala] C86A/C119A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C86A/C119A'. Wed May 02 18:21:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys86ala,cys119ala' Tue Jun 30 16:33:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys86Ala;Cys119Ala]' ecolx Escherichia coli direct interaction direct interaction apk1-3 2 4 unspecified role unspecified role unspecified role unspecified role Region 78-276 Region 78-276 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli disulfide bond disulfide bond disulfide bridge SS-bond